Volume 86  ·   Issue 34  ·  2006

The irritation symptoms caused by gastroesophageal reflux and reflux in patients with gastroesophageal reflux disease (GERD) involve not only the esophagus, but also the external part of the esophagus. Some patients have not been diagnosed and treated for a long time due to atypical manifestations. At the same time, due to the chronic course of GERD, many patients need to receive long-term treatment, which is expensive. Epidemiological studies have shown that GERD is a common disease that affects human health [1]. GERD affects the health and quality of life of people of all ages, and it has been a continuous research hotspot in the world for many years. Experts from all over the world have repeatedly made consensus on the diagnosis and treatment of GERD and formulated diagnosis and treatment guidelines, and continued to revise the diagnosis and treatment guidelines on the basis of evidence-based medicine. In 2005, the diagnosis and treatment guidelines of GERD in the United States [2], both the diagnosis and treatment of GERD emphasized the role of acid suppression, especially proton pump inhibitors (PPI). Although GERD is an upper gastrointestinal motility disorder, so far, there has been no breakthrough progress in the treatment of GERD. At present, it is still aimed at its attack factor, Especially PPI as its main treatment means will have positive significance for the in-depth research of GERD pathogenesis and the exploration of effective treatment methods.

With the acceleration of life rhythm and the increasing pressure of work spirit, the incidence of gastrointestinal functional diseases is on the rise, accounting for more than 40% of the outpatient number of gastroenterology department, which seriously affects the quality of life and work of patients. Some patients seek medical treatment everywhere and spend a lot of medical resources. Therefore, the research of gastrointestinal motility has become a hot spot in the field of digestive diseases nowadays. With the application of computer in the field of gastrointestinal electricity and motor research, gastrointestinal motility detection system and electrogastrogram are more simple and quick in clinical application. It is irreplaceable by all gastrointestinal examination methods (including endoscopy) at present. It has specific and unique diagnostic value for some common clinical diseases such as functional dyspepsia, diabetic gastroparesis, postoperative gastroparesis, pseudointestinal obstruction, reflux gastroesophageal disease, achalasia, gastric dysrhythmia syndrome, constipation, unexplained nausea, vomiting and Hirschsprung's disease, etc. Moreover, gastrointestinal motility detection has developed to not only be limited to gastroenterology, but also involves cardiology, respiratory department, pediatrics, obstetrics and gynecology, surgery and aviation diseases, such as non-cardiogenic chest pain, asthma, chronic cough, chronic pneumonia and other diseases may be caused by reflux gastroesophageal disease.

Objective to study the surgical technique and clinical effect of primary combined transplantation of liver, pancreas, duodenum and kidney in the treatment of post-hepatitis liver cirrhosis complicated with uremia and insulin-dependent diabetes.Methods In January 2005, a patient with post-hepatitis liver cirrhosis complicated with uremia and insulin-dependent diabetes.The liver transplantation was performed by orthotopic piggyback method.The exocrine of the transplanted pancreas was drained by jejunum, and the transplanted kidney was placed in the left iliac fossa.Immunosuppression was induced by anti-CD25 monoclonal antibody and quadruple immunosuppression by anti-thymoglobulin (ATG) + tacrolimus (FK506) + mycophenolate mofetil (MMF) + hormone. Results The function of the transplanted liver and pancreas recovered within 10 days, but the renal function recovery was delayed. On the 16th day after operation, due to the sudden decrease of blood flow in renal cortex, the transplanted kidney was removed and the renal function was restored to normal for the second time. Conclusion Combined transplantation of liver, pancreas, duodenum and kidney with orthotopic piggyback and jejunal drainage of pancreatic juice is a safe technique for the treatment of liver cirrhosis complicated with uremia and insulin-dependent diabetes.

Objective to construct recombinant BCG vaccine (Rbcg) secreting human interferon α-2a (IFN α-2a). Methods the signal peptide sequence of Ag85B was amplified from the genome of BCG by polymerase chain reaction (PCR), and the full-length sequence of IFN α-2aCDNA was amplified from pBIFN α-2a plasmid.The two fragments were inserted into the downstream of the HSP60 promoter of Mycobacterium tuberculosis plasmid Pmv261 by DNA recombination technique to construct a secreted BCG shuttle expression plasmid pMSIFN α-2a. The plasmid pMSIFN α-2a was transferred into BCG to obtain Rbcg by electroporation technique. The expression of IFN α-2a DNA and protein in Rbcg cells and culture supernatant were detected by PCR amplification and Western blot, and the content of IFN α-2a in Rbcg culture supernatant was detected by enzyme-linked immunosorbent assay (ELISA). Results The results of enzyme digestion, PCR amplification and DNA sequencing showed that the cloned signal peptide DNA fragment and IFN α-2a fragment were completely consistent with the results in literature, the ligation direction of pMSIFN α-2a was correct, and the reading frame was completely consistent with the expected results. The gene fragment of IFN α-2a was amplified by PCR using Rbcg plasmid DNA as template. Western blot confirmed that the protein secreted by Rbcg could bind specifically to human IFN α-2a antibody. The high content of IFN α-2a (324.57 pg/ml) in Rbcg culture supernatant was detected by ELISA. Conclusion The constructed Rbcg can secrete functionally active cytokines

Objective to investigate the mechanism of proteasome inhibitor bortezomib inducing apoptosis in myeloid leukemia cells HL60. Methods Apoptosis was confirmed by tetramethylazolium salt (MTT) assay, Hoechst33342 staining and flow cytometry. The expressions of B cell lymphoma/leukemia-2 (Bcl-2), Caspase-9, Caspase-3 and poly-ADP-ribose polymerase (PARP) protein were detected by Western blot. Results the inhibitory effect of bortezomib on the proliferation of HL60 cells was concentration-and time-dependent. 30 nmol/L bortezomib inhibited the proliferation of HL60 cells for 24 hours, the inhibition rate was 76%, and the morphological observation showed obvious nuclear aggregation, pyknosis and fragmentation; Flow cytometry showed that the apoptosis rate was 62.6%; Conclusion Bortezomib can induce apoptosis in myeloid leukemia cells HL60, and its mechanism is related to the inhibition of Bcl-2 protein and the activation of Caspase apoptosis signaling pathway.

Objective to investigate the effects of overexpression of tissue matrix metalloproteinase-2 (TIMP-2) on the proliferation and invasion ability of leukemic SHI-1 cells in nude mice. Methods the expression of TIMP-2 on the membrane surface of SHI-1 cells was detected by flow cytometry. The proliferation and invasion ability of SHI-1 cells with high expression of TIMP-2 in vitro were detected by tetramethylazo salt colorimetric assay (MTT assay) and invasion assay across artificial basement membrane in co-culture with bone marrow stromal cells of leukemic patients. Some nude mice were sacrificed on the 30th day after tail vein inoculation to detect the infiltration and growth of SHI-1 cells in various organs. Results the expression of TIMP-2 on the membrane surface of SHI-1/TIMP-2 cells was 99.3%±0.1%, which was significantly higher than that of SHI-1/MSCV cells (85.9%±2.6%,P<0.05), and the mean fluorescence intensity was also increased 2.06 times. The proliferation and invasion ability of SHI-1/TIMP-2 cells in vitro were stronger than that of SHI-1/MSCV cells The average survival time of nude mice inoculated with SHI-1/TIMP-2 cells was 33.7 days, while that of nude mice inoculated with SHI-1/MSCV cells was 40 days. The difference in average survival time between the two cells was statistically significant, and the infiltration and metastasis phenomenon in vivo was significantly enhanced.Conclusion SHI-1/TIMP-2 cell line with high TIMP-2 expression on membrane surface has stronger proliferation, infiltration and metastasis ability than SHI-1/MSCV cells.

Objective to evaluate the accuracy of intracavitary ultrasound (ELUS) in the diagnosis of invasive depth of rectal cancer and to explore its influencing factors.Methods 117 patients with rectal cancer without radiotherapy and chemotherapy were examined by intracavitary ultrasound before operation, and the staging diagnosis of invasive depth was performed according to TNM staging standard, and the results were compared with the postoperative histopathological results; Results the total accuracy of intracavitary ultrasound was 76.9% (90/117), and the sensitivity of pT1, pT2, pT3 and pT4 were 87.5% (7/8), 51.7% (15/29), 85.7% (60/70) and 80.0% (8/10), respectively. A total of 27 cases were misdiagnosed, of which 14 cases were too deep judgment and 13 cases were too shallow judgment. The sensitivity of intracavitary ultrasound in the diagnosis of pT2 was the lowest, and 13 cases of 14 cases were misdiagnosed as UT 3; Intracavitary ultrasound for pT2 rectal cancer with tumor invasion depth, inflammation or fibrosis depth>1/3 of the muscle layer is easy to judge too deeply and too shallowly. In 13 cases, 7 cases of tumor were located in the upper rectum, and 4 cases of tumor caused obvious intestinal lumen stenosis. The misdiagnosis rate of intracavitary ultrasound for tumor invasion depth in middle and lower rectum was 18.5% (17/92), and that for tumor in upper rectum was 40.0% (10/25). The difference of misdiagnosis rate between the two groups was statistically significant (P =0.024). Conclusion The overall accuracy of intracavitary ultrasound for the diagnosis of rectal cancer invasion depth is high, but there are possibilities of too deep and too shallow judgment. Too deep judgment is an important factor affecting the diagnostic accuracy of pT2 rectal cancer, and inflammation, fibrosis and tumor involvement depth may be related to too deep judgment; The tumor is located in the upper rectum or the intestinal lumen is significantly narrowed as the common causes of overly shallow judgment.

Objective to observe the lipid-lowering efficacy and safety of niacin sustained-release tablets alone and combined with atorvastatin in patients with coronary heart disease and other dangerous diseases.Methods 110 patients with coronary heart disease and other dangerous diseases with serum total cholesterol (TC) ≥3.5 mmoL/L were divided into 3 groups: (1) niacin sustained-release tablets group (n =38), given niacin sustained-release tablets 500 mg/d, and increased to 1000 mg/d after 4 weeks; (2) The atorvastatin group (n =38) was given atorvastatin 10 mg/d; (3) The patients in the combination treatment group (n =34) were given atorvastatin 10 mg/d and nicotinic acid sustained-release tablets 500 mg/d, and the dosage of nicotinic acid sustained-release tablets was increased to 1000 mg/d after 4 weeks of intervention for 8 weeks, and the blood lipid levels were measured and the adverse reactions were evaluated before the intervention, 4 weeks and 8 weeks after the intervention, respectively.Results (1) After 8 weeks of intervention, the triglyceride (TG) decreased by 30% and the high density lipoprotein-cholesterol (HDL-C) increased by 16% (all P<0.05); In the atorvastatin group, TC, low density lipoprotein-cholesterol (LDL-C) and TG decreased by 19%, 26% and 17%, respectively, after 8 weeks of intervention (all P<0.05). After 8 weeks of intervention, TC, LDL-C and TG in the combination treatment group decreased by 28%, 38% and 39%, and HDL-C increased by 23% (all P<0.05). The decrease of TC and LDL-C in the combination treatment group was significantly better than that in the monotherapy group (all P<0.05). (2) The achievement rate of LDL-C in the combination treatment group was 73.5%, which was significantly higher than that in the monotherapy group (all P<0.05). (3) The adverse reactions in the nicotinic acid sustained-release tablets group were more common: flushing (15.8%) and digestive tract symptoms (23.7%), while the adverse reactions in the combination treatment group did not increase, and there were no serious adverse reactions in each group. Conclusion Nicotinic acid sustained-release tablets have good lipid-regulating effects, especially in reducing TG and increasing HDL-C.

Objective to observe the effect of irbesartan, a domestic angiotensin type 1 receptor antagonist, on restenosis (RS) after balloon injury in rabbits, and to explore the possible mechanism of RS. Methods 48 large-eared white rabbits were randomly divided into experimental groups on 7, 14 and 28 days, and a control group (8 in each group) was established. The restenosis model was established by balloon injury of left common carotid artery. The rate of nuclear factor-κ B (NF-κ B) and nuclear proliferation antigen (PCNA) translocation positive cells was determined by immunohistochemistry; The expressions of kappa B inhibitory protein (I-κ B) and monocyte chemoattractant protein mRNA were determined by real-time fluorescence quantitative polymerase chain reaction (PCR). Results Compared with the control group, the intimal hyperplasia was significantly inhibited by irbesartan at 7 days after operation (0.34 mm2±0.15 mm2 vs 1.05 mm2±0.38 mm2,P<0.05), and the lumen area was significantly larger at 28 days after operation (4.25 mm2±0.29 mm2 vs 2.56 mm2±1.02 mm2,P<0.05). Irbesartan significantly inhibited NF-κ B p65 nuclear translocation (P<0.05) and PCNA expression (P<0.05) at 7 days postoperative.The mRNA content of I-κ B in the irbesartan experimental group was significantly lower at 7 days postoperative.The mRNA content of I-κ B in the irbesartan experimental group was significantly lower (7.2 copies/μ L ± 0.9 copies/μ L vs 15.6 copies/μ L ± 0.7 copies/μ L, P<0.05), and the mRNA expression of MCP-1 in the irbesartan experimental group was significantly inhibited at all time points (P<0.05). Conclusion Irbesartan can regulate the expression of MCP-1 and PCNA by inhibiting the activation of NF-κ B and effectively inhibit the development of restenosis.

Objective to observe the effects of hypobaric hypoxia on gastric emptying and small intestinal motility, and to provide theoretical basis for clarifying the effects of flight factors on gastrointestinal motility. Methods The gastric emptying was measured by isotope 99Tcm-SC, and the small intestinal propulsion rate was measured by charcoal powder method. Eight groups of rats (10 rats in each group) stayed at sea level of low pressure chamber and simulated elevation to 3000 m and 5000 m for 30 min, respectively. The gastric emptying rate and small intestinal propulsion rate of rats were measured, and the promotile drug mosapride was given to observe the effects on gastrointestinal motility at high altitude. Arterial blood was collected from each group to measure the plasma motilin and nitric oxide concentrations. Results At the simulated 5000 m altitude, the gastric emptying rate and small intestinal propulsion rate of rats were 41% ± 10% and 37% ± 8%, respectively, which were significantly lower than those in the ground group (62% ± 12% and 61% ± 13%, respectively, both P<0.01); The simulated height of 3000 m had no obvious effect on gastric emptying and small intestinal propulsion rate in rats; The gastric emptying rate in the oral mosapride group at 5000 m height was significantly higher than that in the 5000 m control group (55%±12%vs 40%±10%, P<0.05), but there was no significant effect on the small intestinal propulsion rate; The concentration of plasma motilin was significantly lower than that in the ground group (88 pg/ml ± 19 pg/ml vs 123 pg/ml ± 28 pg/ml, P<0.01), while the concentration of plasma nitric oxide was significantly higher than that in the ground group (106 μ mol/L ± 24 μ mol/L vs 80 μ mol/L ± 18 μ mol/L, P<0.01). Conclusion Acute hypobaric hypoxia inhibits gastric emptying and small intestine propulsion, and promotile drug mosapride can reduce the effect of hypobaric hypoxia on gastric emptying.

Objective to investigate whether six kinds of conjugated bile salts and their mixtures inhibit the proliferation and induce the differentiation of normal esophageal mucosal cells cultured in vitro.Methods 20~50 μ mol/L of six kinds of conjugated bile salts [glycine bile salt (GC), glycine chenodeoxycholine salt (GCDC), glycine deoxycholine salt (GDC), taurine bile salt (TC), taurine chenodeoxycholine salt (TCDC), taurine deoxycholine salt (TDC)] and their mixtures were used to interfere with normal esophageal mucosal cells cultured in vitro for different times (1, 3 and 5 days) respectively; The cell proliferation was observed by tetramethylazo salt (MTT) assay; Analysis of Cell Cycle Changes by Propidium Iodide Staining Flow Cytometry; Cytokeratin (CK) was detected by immunochemistry, and the percentage of CK13 positive cells was observed to observe cell differentiation; The changes of intracellular calcium ion after bile salt induction were detected by laser confocal microscopy. Results GC had no effect on cell proliferation, but other bile salts inhibited cell proliferation; The percentage of CK13-positive cells increased and the percentage of CK14-positive cells decreased at 1, 3 and 5 days of intervention, except for GC, while the percentage of CK13-positive cells increased with the increase of intervention time. Among them, TC had the strongest effect [the percentage of CK13 positive cells in the control group was 22%±7% on day 5, while that in the TC group was 74%±8% (P<0.01)]; Conclusion GDC, GCDC, TC, TDC, TCDC and mixed bile salt can promote cell differentiation and inhibit cell proliferation, but GC does not have this effect. TC and mixed bile salts can cause the increase of intracellular Ca2+, that is, Ca2+ participates in this intracellular signaling pathway that induces cell differentiation.

Objective to investigate the role of gastroesophageal junction (EGJ) barrier function in the pathogenesis of gastroesophageal reflux disease (GERD). Methods 96 patients with typical gastroesophageal reflux symptoms underwent gastroscopy. 42 patients with reflux esophagitis (RE) were divided into grades A ~ D according to LA standard, including 31 cases in groups A and B and 11 cases in groups C and D. Fifty-four patients with NERD were subjected to 24-h esophageal pH monitoring, and they were divided into pH negative group and pH positive group according to DeMeester score. Among them, 31 patients were subjected to pH negative group, 23 patients were subjected to pH positive group and 18 patients were subjected to healthy control group. All subjects were subjected to esophageal pressure measurement, and the observation indexes included EGJ function and esophageal body systolic pressure. The EGJ function indexes included lower esophageal sphincter pressure at rest (LESP), diaphragmatic foot pressure during calm breathing (CDP), EGJ pressure (EGJP) and lower esophageal sphincter relaxation pressure (post-LESRP). Results LESP, CDP, EGJP and post-LESRP in RE group were lower than those in NERD group (all P<0.05); LESP [(9 ± 3) mm Hg, 1 mm Hg =0.133 kPa] and post-LESRP [(39 ± 15) mm Hg] in NERD group were lower than those in healthy control group [(16 ± 4) mm Hg, (50 ± 15) mm Hg, both P<0.05], but CDP [(18 ± 6) mm Hg] And EGJP [(28±8) mm Hg] compared with the healthy control group, the differences were not statistically significant [(16±6) mm Hg, (32±7) mm Hg, all P>0.05]; The distal systolic blood pressure of the body of the esophagus in the NERD group and the RE group was lower than that in the healthy control group, but the difference between the two groups was not statistically significant (both P>0.05). There was no statistically significant difference between the esophageal motility function indexes in LA-C and D groups and LA-A and B groups, and the esophageal motility function indexes of NERD patients in the 24-h esophageal pH monitoring negative group and the pH monitoring positive group were also not statistically significant (both P>0.05).

A 25-year-old female was admitted to the hospital on September 15, 2005 due to pale complexion and fatigue for more than 4 months, and skin stasis for 3 days. Before more than 4 months, the patient felt pale complexion and easily fatigued after activity. On June 21, 2005, routine blood test showed that hemoglobin was 48g/L, mean erythrocyte volume (MCV), mean erythrocyte hemoglobin (MCH) and mean erythrocyte hemoglobin concentration (MCHC) were 51.5 fl, 14.3 pg and 279g/L, respectively, showing microcyte hypochromic anemia. White blood cells and platelets were normal; Serum iron 2.0 μ mol/L, ferritin 9 μ g/L, total iron binding capacity (TIBC) 77.1 μ mol/L, transferrin saturation (ISat) 2%, in accordance with the diagnosis of iron deficiency anemia (IDA). 　　

At present, the incidence rate of malignant tumor has risen to the second place in the disease spectrum in China, and with the influence of environmental pollution, population aging and other factors, it is still increasing. Malignant tumor is seriously endangering the health of our people and hindering the development of our society and economy. However, the research on malignant tumor has not made a fundamental breakthrough yet, and the problems of what is the essence of malignant tumor and how to effectively control its occurrence and development are still being explored.

Dieulafoy disease, also known as gastric submucosal constant diameter artery rupture, is one of the rare causes of gastrointestinal bleeding, but it is not uncommon. With the popularization and application of electronic fiber endoscope and the in-depth and sufficient understanding of the disease by medical workers, the reports at home and abroad are increasing in recent years. The disease has a sudden onset and a high mortality rate, so it is very important to choose a correct diagnosis and treatment method at the first time of onset. At present, endoscopic and surgical resection are still widely recognized by scholars at home and abroad for the diagnosis and treatment of this disease. In this study, 7 cases of Duchenne disease patients with massive bleeding who were ineffective by endoscopy and/or medical medication were successfully treated by digital subtraction angiography (DSA) diagnosis and interventional therapy in emergency department. The reports are as follows.

Upper urinary tract stones are seriously damaged to renal function. In the past, open surgery caused serious damage, and complications such as hemorrhage, secondary hematuria, infection and fever were easy to occur during and after operation, and the postoperative recovery was slow. Extracorporeal shock wave lithotripsy (ESWL) often needs to be repeated many times for the treatment of upper urinary tract stones, which not only leads to renal interstitial hemorrhage and aggravates renal parenchymal damage, but also easily causes ureteral stones. We used percutaneous nephroscopic pneumatic lithotripsy combined with ultrasonic lithotripsy under the guidance of B-ultrasound to treat 78 cases of upper urinary tract stones with 85 sides, all of which were successful, and the clinical results were good.

Myocardial infarction can lead to loss of myocardial tissue and decline of left ventricular function, which is one of the main causes of heart failure. Repairing scar myocardium is of great significance for the treatment of heart failure. Although it has been found that myocardial injection of bone marrow stem cells can promote myocardial regeneration, it is difficult to reconstruct sufficient myocardial volume in the thinning scar area of infarction. Mesenchymal stem cells (MSC) are pluripotent adult stem cells existing in bone marrow microenvironment. Besides Gergenbauer cells, chondrocytes, neurons and skeletal muscle cells, they can also differentiate into vascular endothelial cells and cardiomyocytes. MSC can be isolated from adipose tissue. Scholars led by Yoshinori Miyahara of Osaka National Cardiovascular Center Research Institute isolated and cultured MSC from adipose tissue and used cell plate technology to study the feasibility of monolayer MSC for myocardial remodeling after myocardial infarction. The results of the research were published recently in Nature Medicine (2006, 12: 459-465).

Lipid storage in the liver changes with the change of energy metabolism in the whole body, but how the liver transmits information to other tissues is still unclear. Recently, tissue-specific genetic engineering animal model studies have found that the pairing between tissues is important in the regulation of energy metabolism. Tissue-specific knockout of peroxisome proliferator-activated receptor γ (PPAR γ) gene in mice is a tool to study the interaction between tissues. Liver-specific knockout of PPAR γ gene reduces liver fat deposition, but increases peripheral tissue fat deposition and reduces insulin sensitivity of muscle and adipose tissue.

After acute myocardial infarction (AMI), ventricular remodeling will occur in infarcted and non-infarcted areas [1], eventually leading to heart failure. Early reperfusion therapy has been proven to recanalize infarction-related arteries (IRA) and prevent ventricular remodeling [2]. However, the elderly patients with AMI often delay the diagnosis time due to untypical symptoms, or there are many diseases that are not suitable for thrombolysis or failure of thrombolysis, or the hospital where they are located does not carry out direct coronary intervention (PCI), so they miss the best time for early reperfusion of myocardial infarction [3]. Whether delayed reperfusion can recanalize IRA can also improve ventricular function is still controversial. This paper discusses the effect of delayed PCI on improving ventricular remodeling by detecting the changes of plasma brain natriuretic peptide concentration, and provides theoretical basis and objective index for it.

Allogeneic penile transplantation has not been reported at home and abroad. We recently successfully carried out a case of allogeneic penile transplantation, which is reported as follows.

Receptor desensitization refers to the decrease of tissue or cell sensitivity to agonists after the use of receptor agonists. G protein-coupled receptors mostly exist desensitization phenomenon, and H2 receptor is a member of this family. Studies have shown that H2 receptor desensitization phenomenon exists in many kinds of cells [1-3], and no research on H2 receptor in gastric parietal cells has been seen yet.

Vascular cognitive impairment (VCI) is a multifactorial syndrome. Studies have confirmed that the rat models of bilateral forebrain ischemia or middle cerebral artery ischemia have atrophy of hippocampal CA1 region and delayed neuronal death [1, 2]; The autopsy of small vessel disease dementia also confirmed a large number of cone cell loss in the hippocampal CA1 region [3]. Based on this, combined with magnetic resonance imaging (MRI) and MRS methods, the morphological and pathological biochemical changes of VaD hippocampus were studied in patients with clinically suspected vascular dementia (VaD) and normal controls.

The role of interventional surgery in the treatment of vascular complications after liver transplantation has been paid more and more attention, and there have been case reports that interventional surgery can effectively treat hepatic artery thrombosis [1,2]. However, there are few successful reports of interventional surgery in the treatment of hepatic artery stenosis or occlusion at home and abroad.

A 37-year-old male was diagnosed with Ewing sarcoma in the soft tissue of the right upper arm for more than 2 months in October 2004 because of painless mass in his right upper arm. He was admitted to our hospital for more than 1 month because of pale complexion, stasis and ecchymosis on the skin in May 2005. He was diagnosed with acute myeloid leukemia type M1 (AML-M1) by bone marrow cytology and immunotyping. He was treated with medium-dose cytarabine for one course of treatment to achieve complete remission, followed by consolidation treatment. In August 2005, he was admitted to hospital for allogeneic hematopoietic stem cell transplantation. The pretreatment plan was modified busulfan/cyclophosphamide (BU/CY2). Cyclosporine combined with short-course methotrexate was used to prevent acute graft versus host disease (aGVHD). In November 2005, the blood routine examination of leukocytes 3.8×109/L, hemoglobin 86g/L and platelets 165×109/L showed that the STR genotypes of the blood samples before and after transplantation were changed, and the 12 STR genotypes such as FGA after treatment were consistent with those of the donor.