Volume 86  ·   Issue 33  ·  2006

Minimally invasive thoracic surgery, represented by video-assisted thoracoscopic surgery, has become a mature commonly used surgical technique in China after more than ten years of rapid development. The popularity of thoracoscopic surgery in China has also developed from central cities and developed areas to central and western regions in the past, and the difficulty and application ratio of the surgery have also increased greatly. In some hospitals with better conditions, thoracoscopic surgery has accounted for about half of the thoracic surgery volume.

Since Shen Gongen et al. [1] first reported video-assisted thoracoscopic thoracic sympathicotomy (ETS) for the treatment of hand hyperhidrosis in 1994, the number of units and surgical cases that have carried out ETS in China has increased year by year, the surgical technique has become increasingly mature, the surgical effect has been more satisfactory, the patients' hands have been kept warm and dry for a long time after operation, and the accompanying hyperhidrosis symptoms of head, face, axillary and plantar have mostly disappeared or obviously reduced. Practice has proved that ETS is indeed a safe and effective minimally invasive method for the treatment of hand hyperhidrosis. However, like other surgeries, ETS will also have complications, even life-threatening complications such as sudden cardiac arrest [2]. Therefore, we must not take ETS lightly because it is a minimally invasive operation, and should attach great importance to the prevention and treatment of surgical complications.

Objective to investigate the neuroprotective effects of glial growth factor 2 (GGF2) on craniocerebral injury in rats with hydraulic attack. Methods the rat model of craniocerebral injury was prepared by lateral hydraulic attack device. The expression plasmid of pEGFP-N1-GGF2 or pEGFP-N1 vector plasmid was mixed with cationic liposomes, and then the brain tissues of rats were transfected directly into the brain. 34 rats were randomly divided into 4 groups: treatment group (pEGFP-N1-GGF2+ liposome, n =10), carrier control group (pEGFP-N1 vector + liposome, n =10), liposome control group (liposome, n =10) and sham operation group (n =4). After injury, the climbing, balance and walking tests were continuously observed. The brain tissues of the rats were taken by sacrifice on the 10th day after injury, and immunohistochemical staining was performed with HE, Nissl, MBP, NSE and GFAP. Results On the 5th day after injury, the recovery of behavioral indexes in the treatment group was better than that in the carrier control group and the liposome control group [climbing test (angle): 66.25±3.54 vs 58.31±3.54 The neuronal counts in the cortex and hippocampus of rats in the treatment group were higher than those in the carrier control group and the liposome control group (extracortical granule and outer pyramidal layer: 98 ± 10 vs 75 ± 7, 67 ± 8, P<0.05; intracortical pyramidal layer: 37 ± 4 vs 19 ± 3, 23 ± 4, P<0.05; hippocampal CA1 area: 102 ± 11 vs 67 ± 8, 58 ± 9, P<0.01), and the MBP staining signal in the cortex and subcortical white matter was stronger than that in the control group. Conclusion Cationic liposome-mediated GGF2 gene therapy can effectively promote the recovery of rats after craniocerebral injury.

Objective to better understand the carrying status of hepatitis B virus in human body and guide the clinical diagnosis and treatment by detecting serological markers of hepatitis B virus (HBsAg, anti-HBs, HBeAg, anti-HBe, anti-HBcIgG) and comparing the internal standard and external standard polymerase chain reaction (PCR) methods for detecting hepatitis B virus viral load. Methods After treatment, the serum of 42 cases with normal alanine aminotransferase (ALT) /aspartate aminotransferase (AST), undetectable HBV-DNA by COBAS AMPLICOR quantitative PCR (internal standard method) and positive HBsAg, negative surface antibody (anti-HBs), positive e antigen (HBeAg>4 S/CO), negative HBe and positive HBcIgG were detected by Abbott AxSYM microparticle immunoassay (MEIA). HBV-DNA was detected by Light Cycler quantitative PCR (external standard method), and 7 cases were positive. The average content of HBV-DNA in 7 cases was 3.1×105 copies/ml, and the positive rate was 17%. Conclusion the positive HBV e antigen does not indicate that the virus replication in vivo is definitely active. After treatment, the virus replication decreased, and some patients remained positive for e antigen. When the e antigen disappeared needs further research. There is inconsistency between serological markers and HBV replication status, and it is more objective to detect HBV-DNA by taking into account both internal and external standard PCR methods.

Objective to compare the quantity of fetal cells obtained from different parts of the cervix in early pregnancy, so as to determine the better sampling location.Methods The irrigation fluids from the internal and external cervical orifices of women with induced abortion at 5~10 weeks of gestation were collected, which were called internal and external irrigation groups, respectively, 60 cases in each group.The DNA in the irrigation fluid was extracted, and the SRY gene of Y chromosome was amplified and quantified. The karyotype of the villi after abortion was directly analyzed to determine the sex of the aborted fetus.Results (1) In the internal cervical irrigation group, 71.4% (15/21) of the known male fetuses and 5.13% (2/39) of the known female fetuses were detected by real-time quantitative PCR. The total sex prediction rate was 86.7%; The SRY gene of 37% (10/27) male fetuses and 6.25% (2/32) female fetuses were detected by real-time quantitative PCR. The total gender correct prediction rate was 67.8%. The total gender correct prediction rate and the male fetal positive rate of the inner cervical irrigation group were higher than those of the outer cervical irrigation group, and the difference was statistically significant (P<0.05). (2) The copy number of SRY gene in the DNA extract of the inner cervical irrigation group was 1487.2±430.5 by real-time quantitative PCR, which was higher than that of the outer cervical irrigation group (702.4±365.7 copies), and the difference was statistically significant (P<0.05). Conclusion The fetal cells obtained through the cervix can provide a possible way for non-invasive prenatal diagnosis in early pregnancy, and more fetal cells can be obtained through the inner cervical orifice than through the outer cervical orifice, but a safer and more effective method should be adopted.

Objective to evaluate the clinical value of three clinical treatment schemes in patients with atypical squamous cells (ASC-US) with unclear cervical cytopathology.Methods According to the diagnosis and treatment standards proposed by TBS system and American Society of Colposcopy and Cervical Pathology (ASCCP) in 2001, the value of three clinical treatment schemes was evaluated.1394 cases of ASC-US diagnosed by cervical cytopathology (liquid-based thin layer) were divided into three groups to evaluate the treatment of three clinical treatment schemes; The percentage of histopathological findings ≥ high grade squamous intraepithelial lesion (HSIL), i.e. ≥ cervical intraepithelial neoplasia Ⅱ (CIN2), was evaluated as the criterion. Group A: 421 cases, direct colposcopy + biopsy/cervical curettage (ECC); Group B: 475 cases, followed up after 6 months, repeated cytopathological examination results ≥ ASC-H or ASC-US and HPV-DNA positive patients underwent colposcopy + biopsy/ECC.Group C: 498 cases, HPV-DNA detection, HPV-DNA positive patients were divided into ≥30 years old,<30 years old, respectively, colposcopy + biopsy/ECC.Histopathological results ≥ CIN2, group A: 27 (6.41%), group B: 26 (5.78%), and group C: 34 (6.91%), the difference between the three groups was not statistically significant (P>0.05), the difference between the three groups was statistically significant (P<0.01), that is, the workload of group A was heavy, the patients had discomfort; Group B had long waiting time and poor compliance. In terms of cost, Group C was more expensive (P<0.01). In Group C, 66% of ASC-US and HPV-DNA positive patients were ≥30 years old, and 34% were<30 years old. Histopathological CIN2, the former accounted for 5.69%, and the latter accounted for 1.22%. Conclusion Scheme B and C are more practical in clinic.Because the cause of cervical cancer is HPV infection, if economic conditions permit, Scheme C should be the first choice, that is, ASC-US patients should be tested for HPV-DNA at the same time.In ASC-US, HPV-positive patients over 30 years old are the high risk groups for screening.

Objective to investigate the significance of citrullinated bovine collagen type Ⅱ and its antibody in the pathogenesis and diagnosis of rheumatoid arthritis (RA). Methods Peptidylarginine deiminase (PAD) was used to catalyze the conversion of arginine into citrulline in bovine collagen type Ⅱ (bCⅡ). The serum antibody levels of citrullinated bovine collagen type Ⅱ (Cit-bCⅡ) were detected by ELISA and immunoblotting in 175 patients with RA, 112 patients with systemic lupus erythematosus (SLE), 37 patients with osteoarthritis (OA) and 160 healthy controls. The relationship between the antibody and anti-cyclic citrulline (CCP) antibody, C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), rheumatoid factor (RF), X-ray stage of both hands, course of disease and age was studied. Results the positive rate of anti-cit-BCⅡ antibody was 50.3% (88/175), which was significantly higher than that of 33.7% (59/175). There was no significant difference between anti-CIT-BC Ⅱ antibody and anti-CCP antibody in RA patients, but there was no significant correlation with CRP, ESR, RF, X-ray stage of both hands, course of disease and age. Conclusion Citrullinated CⅡ and its antibody may play an important role in the pathogenesis and pathological process of RA.

Objective to construct a subtractive cDNA library of the bone tissue of the facet articular processes of juvenile idiopathic scoliosis in order to study the gene expression changes of idiopathic scoliosis. Methods the mRNA was extracted from the bone tissue of the convex and concave vertebrae of the apical vertebrae of idiopathic scoliosis patients. After reverse transcription, the subtractive cDNA library of the differentially expressed genes between the two tissues was constructed by suppression subtractive hybridization (SSH) method by two rounds of hybridization and two times of suppression PCR. The differentially expressed genes were screened and sequenced, and the results were compared with the nucleic acid database. Furthermore, the differentially expressed genes were verified by immunohistochemistry and in situ hybridization methods in 11 patients with idiopathic scoliosis. The obtained immunohistochemistry and in situ hybridization images were input into the image analysis system for semi-quantitative analysis. Results The sequencing results of 600 clones were preliminarily analyzed, and the protein level and gene level were examined, The microglobulin gene (β 2M) and calganulin A gene (S100A8) were obtained. Conclusion A subtractive hybridization method can be used to establish a specific subtractive cDNA library of idiopathic scoliosis spinal facet joint bone tissue under the condition of removing the same genetic background. The library can further screen the gene groups related to the occurrence and development of idiopathic scoliosis in batches, and clone the expressed genes related to idiopathic scoliosis, and study the relationship between them and the molecular mechanism and biological characteristics of idiopathic scoliosis.

Objective to clarify the incidence of intraoperative knowledge in elective surgery using general anesthesia methods at present, analyze the possible causes of intraoperative knowledge, and analyze the postoperative situation of intraoperative knowledge cases. Methods A total of 28 patients (1.4%) were followed up with intraoperative knowledge. Female (OR =2.836; 95% CI 1.181-6.810), used laryngeal mask for airway management (OR =19.609; 95% CI 3.894-98.740), no volatile anesthetic (OR =3.084; 95% CI 1.246-7.629) during anesthesia maintenance, and patients with intraoperative blood pressure fluctuation (OR =10.430; 95% CI 3.918-27.763) were prone to intraoperative knowledge. The use of premedication (OR =0.326; 95% CI 0.110-0.965) helped to reduce the risk of intraoperative knowledge. However, the age, body weight, ASA grade, anesthesia time, combination of block anesthesia and opioid analgesics did not significantly affect the occurrence of intraoperative knowledge. 7/28 (25%) had different degrees of pain during operation; 10/28 (36%) had fear and anxiety during operation; 6/28 (21%) had different degrees of postoperative sequela.Conclusion Intraoperative knowledge can be sporadic in many kinds of elective operations.In females, the use of laryngeal mask for airway management, the use of volatile anesthetic for a period of time or the whole course of anesthesia maintenance, and the fluctuation of blood pressure during the operation are possible risk factors of intraoperative knowledge in general anesthesia.The use of preoperative drugs is beneficial to prevent intraoperative knowledge.Patients with intraoperative knowledge may have different degrees of postoperative sequelas.2.

Objective to summarize the experience of surgical treatment of triangular meningioma of lateral ventricle. Methods the clinical efficacy of 64 patients with triangular meningioma was retrospectively analyzed. Among 64 cases, 40 cases were located on the left side and 24 cases were located on the right side. The pathological types were: fibrous type in 35 cases, mixed type in 10 cases, endothelial type in 8 cases, transitional type in 3 cases, secretory type in 1 case, malignant in 1 case and unclassified in 6 cases. Results the average tumor diameter was 4.8 cm. Total tumor resection was performed under microscope in all cases, and none of them died after operation. The postoperative complications included hemiplegia in 4 cases, aphasia in 14 cases, hemianopia in 2 cases, fever over 1 week or even more than 2 weeks in 30 cases, and localized hydrocephalus in 4 cases. The incidence of postoperative aphasia was related to the site of craniotomy (Pearson's correlation coefficient =0.404, P =0.001), the side of tumor (Pearson's correlation coefficient =0.313, P =0.012), and the diameter of tumor (Pearson's correlation coefficient =0.284, P =0.023). The postoperative fever was related to the diameter of tumor (Pearson's correlation coefficient =0.367, P =0.003), and the resection mode of tumor (Pearson's correlation coefficient =0.537, P =0.000). In cases with tumor diameter ≥4 cm, there was no significant correlation between postoperative hospital stay and ventricular drainage (Pearson's correlation coefficient =0.047, P =0.748). Conclusion Parieto-occipital craniotomy and temporo-parieto-occipital craniotomy are better surgical approaches for resection of meningioma located in triangular area.

Objective to explore the feasibility of T3 and T4 cutting of sympathetic nerve chains at a lower single position for the treatment of hand hyperhidrosis. Methods A multicenter retrospective control study was conducted to collect 103 patients with primary hand hyperhidrosis from September 2004 to February 2006, 49 males and 54 females, with an average age of 23.2 years. 103 patients entered T3 and T4 treatment groups, respectively, and underwent bilateral T3 (n =66) or T4 (n =37) sympathetic nerve chain cutting surgery at the same time. Results All the surgeries were successfully completed without serious complications and perioperative deaths. All patients were followed up for an average of 8.6 months ± 5.2 months. The symptoms of palm hyperhidrosis were completely improved, and the effective rate of the whole group was 100%. In T4 group, 54.1% patients complained that their palms were slightly damp when the weather was too hot, but the patients were satisfied with this result. In T3 group, 15.2% patients had similar symptoms, and the difference between the groups was statistically significant (χ 2=17.38, P<0.01). In T3 group, 25.8% had obvious dry and cracked palms after operation, while in T4 group, 5.4% had similar symptoms, and the difference was statistically significant (χ 2=6.53, P<0.05); Compensatory perspiration occurred in 33.6% of patients, including 30 cases (45.6%) in T3 group and 8 cases (21.6%) in T4 group, with statistically significant difference (χ 2=5.78, P<0.05). Conclusion T3 and T4 amputation at single position in lower segment is effective in the treatment of hand hidrosis, and T4 amputation has less complications, which is more worthy of recommendation.

Objective to investigate the efficacy of different segments of thoracic sympathetomy in the treatment of hand hyperhidrosis and the difference of postoperative compensatory hyperhidrosis.Methods From January 2003 to June 2006, 400 cases of thoracoscopic sympathetomy were performed (2 cases abandoned right side operation due to severe pleural adhesion), and 398 cases were divided into 3 groups.Group A: 207 cases of T2-4 thoracic sympathetomy; Group B: 131 cases of T3 thoracic sympathetic trunk transection; Group C: 60 cases of T3-4 thoracic sympathetic trunk transection. Results All groups were successful, all the symptoms of postoperative palmar hyperhidrosis disappeared, and there were no serious complications after operation. The postoperative compensatory hyperhidrosis in group B and group C was 5.3% and 6.7%, respectively, and the postoperative compensatory hyperhidrosis in group B + C and group A was 5.8% and 28.0%, respectively, with statistically significant differences (χ 2=32.812, P<0.01). The incidence of postoperative compensatory hyperhidrosis in group B + C was significantly reduced. Conclusion T3 or T3-4 thoracic sympathetic trunk transection has reliable curative effect on hand hyperhidrosis, which can effectively reduce the occurrence of postoperative compensatory hyperhidrosis and improve the postoperative quality of life of patients.

Objective to evaluate the long-term effect of thoracoscopic thymectomy in the treatment of myasthenia gravis. Methods the data of patients who underwent thoracoscopic thymectomy in the same hospital in the past 5 years were retrospectively studied. The data were collected from medical records and telephone follow-up.From November 2001 to May 2005, 18 consecutive patients with myasthenia gravis underwent thoracoscopic thymectomy completed by the same surgical group. Among them, 7 males and 11 females, aged 19-48 years, with an average of 33.4 years ± 9.4 years. Most of them underwent thoracoscopic thymectomy and anterior mediastinal adipose tissue debridement through right approach. Results The average postoperative closed drainage was 2.1 days ± 1.2 days, and the average postoperative hospitalization was 6.3 days ± 3.1 days. Ventilator-assisted ventilation accounted for 28% (5/18) of perioperative period, of which 3 cases were short-term ventilator-assisted ventilation (no more than 72 h) after operation, and 2 cases were secondary tracheal intubation due to cholinergic or myasthenia crisis.Two cases were lost to follow-up, and no death.The follow-up time was 26.4 months±10.9 months.According to the criteria established by the American Myasthenia Gravis Foundation (MGFA), the efficacy was judged in 9 cases (50%) with complete stable remission (CSR), 6 cases (33.3%) with partial remission, and 1 case was stable (5.6%). The total effective rate in this group was 83.3%.Conclusion Thoracoscopic thymectomy is safe and reliable in the treatment of myasthenia gravis, and the long-term effect is affirmative, and the efficacy is equivalent to other traditional methods.

Methods 1264 cases of minimally invasive thoracoscopic surgery were performed from December 1993 to December 2005, including 622 cases of bullae resection, 119 cases of mediastinal tumor and cyst resection, 107 cases of esophageal disease surgery, 215 cases of lobectomy or pulmonary wedge resection, 17 cases of emphysema volume reduction surgery, 28 cases of thoracic trauma surgery, 72 cases of other thoracic disease surgery, and 84 cases of thoracic disease biopsy. Among them, video assisted thoracoscopic surgery (VATS) performed esophageal cancer resection through right thoracic free esophagus and lymph node dissection, free stomach through median incision of upper abdomen, and stomach pulled to the neck through esophageal bed for esophageal and gastric anastomosis. Results 1230 cases were operated by VATS or thoracoscopic assisted small incision, 34 cases were converted to traditional thoracotomy due to thoracic adhesion or malignant tumor in order to achieve radical cure. The main complications were 45 cases, accounting for 3.56%. There were 4 cases of postoperative hemorrhage, of which 3 cases were reoperated by VATS to stop the hemorrhage, and 1 case was thoracotomy to stop the hemorrhage; Three cases of postoperative pleural effusion or gas accumulation were placed with closed drainage tube again; Esophageal mucosal rupture occurred in 4 cases of achalasia myotomy and 1 case of esophageal leiomyoma removal, which was repaired immediately; One case of thoracic infection and esophagopleural fistula after operation of esophageal diverticulum; A case of pulmonary infection after volume reduction operation for emphysema; One patient with spontaneous pneumothorax and respiratory failure died of respiratory failure on the fifth day after bullectomy; Conclusions (1) Spontaneous pneumothorax bullae resection and some benign thoracic diseases are the main indications of VATS, and VATS operation for malignant thoracic tumors should be cautious; (2) It is an important link to reduce complications to pay attention to VATS surgical training for thoracic surgeons and master the principle of gradual progress; (3) The choice of thoracoscopic or thoracoscopic-assisted minimally invasive surgical methods should be based on the patient's condition and advocate the principle of individualization.

A 56-year-old male was admitted to the hospital on March 17, 2002 due to blurred vision in both eyes for 2 months. The visual acuity of both eyes was 0.4 on the right and 0.9 on the left, and the bilateral fundus papilla edema and visual field were normal. No other abnormalities were found in the corresponding neurological and ophthalmic examinations. The symptoms were not significantly relieved after treatment of optic nerve nutrition. One month later, he developed low back pain and the visual acuity decreased with a tendency of aggravation.

A 2-year-old female was admitted to the hospital on July 15, 2005 due to polydipsia, polyphagia and abnormal stature for 1 year. She had 7 days of menstrual cramps. She had no history of emaciation, polyuria and epilepsy. Physical examination showed that she was clear, sharp in speech, and had better intelligence than children of the same age. Her pupils were large and equal in round, and she was sensitive to light reflection. Her visual acuity and fundus could not be examined in both eyes. The examination of remaining cranial nerves and nervous system was negative. She was euphoric and hyperactive. Gynecological examination showed that she was similar to the vulvar adult type, with breast development. B-ultrasound showed that the uterus was 2.7 cm ×2.6 cm ×2.0 cm. Height was 108 cm and weight was 21 kg. Estradiol 509.8 pmol/L, progesterone 2.2 nmol/L. Department of Neurosurgery, Affiliated Hospital of Hebei University, Baoding 071000

Since the 1990s, the development of video-assisted thoracoscopic surgery (VATS) has brought unprecedented opportunities and challenges to the diagnosis and treatment of thoracic surgery diseases.Many doctors have made many explorations on the application of minimally invasive thoracic surgery technique represented by VATS in thoracic tumors.Recent literature shows that for stage Ⅰ non-small cell lung cancer, VATS lobectomy/pneumonectomy plus mediastinal lymph node excision/dissection can achieve the 5-year survival rate comparable to traditional thoracotomy [1,2], but some studies have pointed out that VATS lung cancer surgery can increase the potential risk of lung cancer metastasis.With the development of automatic mechanical technology, computer navigation technology and 3D video technology, robot surgery technology that can simulate surgeon's hand operation has emerged as the times require, which may fundamentally solve the limitation of VATS technology and make the surgical treatment of thoracic tumor enter the era of robot.

Smoking is one of the main risk factors for cardiovascular diseases. In recent years, some authors have reported that smoking increases the risk of diabetes in a dose-dependent pattern, but this result has not been repeated by other authors' studies. Scholars such as Assistant Professor Houston TK of Birmingham Veterans Medical Center of the University of Alabama conducted a prospective cohort study involving young people aged 18-30, including African Americans and whites, in four cities, including Birmingham, Chicago, Minneapolis and Oakland, and found that both active and passive smoking increased the risk of developing glucose tolerance abnormalities in young people.<BMJ, 2006, 332: 1064-1069.

An 18-year-old male was admitted to hospital on February 20, 2006 due to intermittent cough accompanied by dull pain in the right chest. A huge mass in the upper lobe of the right lung was found for 9 months, and was admitted to hospital on February 20, 2006. Previously, the patient had been seen in many large hospitals in China, but none of them could confirm the diagnosis. Percutaneous biopsy of tumor was performed, and the results showed that there were a few blood vessels, connective tissue, skeletal muscle and blood clots, and the possibility of teratoma was high. After admission, the patient completed all examinations, and fiberoptic bronchoscopy showed that the extrabronchial pressure in the upper lobe of the right lung changed, but no abnormalities were found. Chest CT findings: a huge mass in the upper right chest cavity, about 18 cm in diameter, with uneven density, with clear edges in part, and adhesion to the lateral chest wall, the top of the pleura and the mediastinum, with unclear boundaries, invaded and blocked the main trunk of the right pulmonary artery, and multiple enlarged and fused lymph nodes in the mediastinum, compressing the superior vena cava and trachea.

Cervical cancer is one of the most common malignant tumors that threaten women's health. Its incidence rate is gradually increasing, and the onset age is getting younger, which has seriously affected the reproductive health of women of childbearing age. Because the occurrence and development of cervical cancer is a gradual process from quantitative change to qualitative change, standardized diagnosis and treatment of cervical lesions has attracted attention. Colposcopy is an effective method for early diagnosis of cervical cancer, which has been widely used in clinical practice. However, at present, colposcopy is still at the level of morphological qualitative diagnosis, and there is still a lack of unified diagnostic standards, and colposcopy often has large subjective factors. Therefore, how to quantify the changes of epithelium and blood vessels to achieve the purpose of quantitative diagnosis and reduce the influence of subjective factors is the current research direction.

Recent studies have shown that Eph family, especially EphB4/EphrinB2, play an important role in tumorigenesis and angiogenesis [1, 2]. There are few reports on the expression of EphB4 and its ligand EphrinB2 in cervical cancer tissues at present. We examined the expression of EphB4 and its ligand EphrinB2 in cervical cancer tissues and analyzed their relationship with microvessel density (MVD), aiming to investigate the role of EphB4 and its ligand EphrinB2 in cervical cancer genesis and progression and their relationship with angiogenesis.

ING1 gene consists of three exons and four spliceosomes, which encode four protein subtypes: p47ING1a, p33ING1b, p24ING1c and p27ING1d. p33ING1b is the most well-known protein subtype [1]. Studies have shown that p33ING1b can act synergistically with p53, and play a role in cell cycle inhibition and apoptosis [2]. At present, there are few reports on the chemosensitivity of ING1 gene in osteosarcoma. This study provides a basis for the combination of p33ING1b and chemotherapeutic drugs in the treatment of osteosarcoma by exploring whether p33ING1b can promote the sensitivity of osteosarcoma cells to paclitaxel and the mechanism of p33ING1b promoting chemosensitivity.

Vascular endothelial growth factor (VEGF) is involved in the pathological process of cerebral ischemia and brain tumor [1]. However, the role of VEGF in the pathological process of bacterial meningitis (BM) is rarely reported at home and abroad. We established a BM model by inoculating Escherichia coli (Ec) with K1 antigen into the cerebral cistern of rabbits through cerebellobulbar cistern puncture, and detected the mass concentration of VEGF in cerebrospinal fluid (CSF) of rabbits with meningitis, aiming to investigate the pathogenic role of VEGF in BM.

The 9th National Conference on Clinical Epidemiology was held in Shanghai from June 14 to June 17, 2006. 261 delegates from all over the country and the Netherlands, Britain, the United States, India and Hong Kong attended the conference. The delegates exchanged information on current hot topics such as clinical epidemiology methodology, clinical research and evidence-based medicine in the form of chapter reports or posters.