Volume 99 ·  Issue 29 ·  2019

This article summarizes the epidemiology, mechanism, clinical manifestation, prevention and clinical management of cervical kyphosis after laminectomy, in order to provide reference for clinical work.

This paper reviews and summarizes the risk factors and preventive measures of cervical kyphosis after posterior cervical decompression, and provides useful reference for the selection and formulation of cervical surgical treatment strategies.

Traumatic cervical kyphosis (TCK) is often delayed and iatrogenic kyphosis, and its surgical treatment aims to adequately decompress to relieve spinal cord compression, prevent the progression of the deformity and reestablish local and overall balance, prevent structural decompensation of adjacent segments, relieve pain, and improve respiratory and swallowing functions and maintain stability. In order to achieve this objective, in addition to the evaluation of the patient's age, bone mineral density, cardiopulmonary and neurological function, a comprehensive and systematic evaluation of the cause of posttraumatic kyphosis, whether it is operated, the site of the deformity, the severity, the degree of stiffness and sagittal balance is needed.

Project Moderator:Wang Tianlong (Department of Anesthesiology, Xuanwu Hospital, Capital Medical University); Wang Dongxin (Department of Anesthesiology, Peking University First Hospital)

To investigate the surgical treatment of severe congenital cervical kyphosis.

The clinical data of patients with severe congenital cervical kyphosis (Cobb>40°) treated in Peking University Third Hospital from March 2004 to March 2018 were retrospectively summarized. In this series, 8 cases were enrolled, included 4 males and 4 females; the patients were 5-45 years old. According to the etiology, 4 patients were diagnosed with vertebral body underdevelopment, 2 with vertebral insufficiency, 1 with cervical spine congenital fusion and 1 with C₂ spinous process mecism. Five cases were treated with traction before final surgical correction. The surgical strategy was anterior correction or posterior correction or combined procedure in regards to different situation. The curvature of cervical angle was measured by two-line Cobb method, and the cervical kyphosis angle was measured on lateral radiographs in the neutral and extended position at the pre-operation and post-operation in each patient. The correction rate and evaluated Japanese Orthopedic Association (JOA) scoring for the function of spinal cord were also measured. The data before and after the operation were compared with t test.

In this series, the average kyphotic Cobb angle was 67°±18° and 8°±8° before and after surgical correction, respectively (t=8.471,P<0.05).The final correction rate was 87%±13%.The JOA score improved from 11.1±2.7 to 14.0±1.5 (t=-2.656, P<0.05) at the end of follow up.

The pre-correction by cervical spine traction and final surgical correction by anterior, posterior or combined approaches of internal fixation and fusion can achieve good results and reduce risk and difficulty in operation for severe congenital cervical spine kyphosis with vertebral body underdevelopment without vertebral insufficiency and cervical spine congenital fusion. The final surgical correction by one-stage anterior, posterior or combined approaches of internal fixation and fusion can achieve good results for severe congenital cervical spine kyphosis with vertebral insufficiency and/or cervical spine congenital fusion.

To investigate the change of esophageal length measured on computed tomography after C₇ pedicle subtraction osteotomy (PSO) for cervicothoracic kyphosis in ankylosing spondylitis (AS) patients and its clinical significance.

Eight male AS patients with cervicothoracic kyphosis, who underwent PSO at C₇ level from December 2014 to November 2018 at Nanjing Drum Tower Hospital, were retrospectively reviewed. The mean age was (35±7) years (range, 26-49 years). The cervicothoracic kyphosis, C₂-T₁ sagittal vertical axis (SVA) and angle of fusion levels (AFL) were measured on lateral cervical radiographs and chin-brow vertical angle (CBVA) was measured on clinical photographs preoperatively and postoperatively. Anterior height of the osteotomized vertebra (AHOV) and esophageal length from the lower endplate of C₆ to the inferior endplate of the lower instrumented vertebrae were measured on sagittal plane of reconstructed computed tomography preoperatively and postoperatively. Oswestry Disability Index (ODI), Neck Disability Index (NDI) and Numerical Rating Scale (NRS) were collected to evaluate the clinical outcomes.

The average follow-up duration was (15±9) months (range, 3-51 months). The average correction of cervicothoracic kyphosis, C₂-T₁SVA, AFL and CBVA was 35.9°±7.3°, (44.7±11.6) mm, 32.0°±4.8° and 38.1°±11.5°, respectively. The average reduction of AHOV was (5.6±1.6) mm. ODI was improved from 17±14 preoperatively to 13±10 at the final follow-up. The NDI before operation and at the final follow-up was 18±15 and 10±6, respectively. The preoperative NRS was 4.8±2.4, and it decreased to 1.0±1.2 at the final follow-up. The change of esophageal length showed significant correlation with the improvement of cervicothoracic kyphosis, C₂-T₁SVA, AFL and CBVA(r=0.84, 0.83, 0.83, 0.73, all P<0.05).

The operation of C₇PSO increases esophageal length after cervicothoracic kyphosis in AS patients. The esophageal elongation is closely related with changes of parameters measured on radiographs and clinical photographs. Spine surgeons should be aware of the potential risk of esophagus-related complications caused by esophagus lengthening after C₇PSO.

To investigate the surgical strategy and mid-and long-term outcomes of neurofibromatosis associated cervical kyphotic deformity.

Thirteen patients with neurofibromatosis associated cervical kyphotic deformity operated in Shanghai Changzheng Hospital from January 1998 to December 2015 were analyzed retrospectively. There were 7 males and 6 females in this group, aged from 12 to 61 years, with an average age of (28±15) years. Eight patients were treated with anterior surgery (Group A) and 5 patients were treated with combined anterior and posterior surgery (Group A+P). Cobb angle correction of cervical kyphosis and improvement of clinical symptoms were followed up. Clinical efficacy between the two groups was compared and analyzed. Chi-square test, Fisher exact test and independent sample t test were used for comparative analysis between the two groups.

All patients were operated successfully and finished follow up. The follow-up period was from 42 to 128 months ((80±22) months). After the surgery, neurological symptoms and pain were significantly improved in all patients. Compared with preoperative values, Japanese Orthopedic Association (JOA) score and visual analogue scale (VAS) score for pain at the last follow-up were significantly improved (t=7.63, -5.19, 8.63, -4.75, all P<0.01). Cervical kyphosis was significantly improved in all patients after surgery. In group A, the Cobb angle was improved from 64°±24° preoperatively to 12°±11° at the last follow-up, and the average correction rate of Cobb angle was 82.6%. In group A+P, the Cobb angle was improved from 55°±10° preoperatively to 7°±9° at the last follow-up, and the average correction rate of Cobb angle was 88.3%. The operation time, intraoperative blood loss and length of stay in group A were all significantly lower than those in group A+P (t=-6.32, -11.92, -6.52, all P<0.01). At the last follow-up, there was no significant difference in Cobb angle, JOA score and VAS score between the two groups (t=0.89, 0.94, 1.02, all P>0.05).

Mid-and long-term results of anterior and combined anterior and posterior surgery for neurofibromatosis associated severe cervical kyphosis are satisfactory. Moderate correction strategy for cervical kyphosis is safe and effective. The incidence of complications of nerve injury can be reduced.

To evaluate the effect of ABO blood groups on long-term outcome of stable coronary artery disease (CAD) after percutaneous coronary intervention (PCI).

A total of 4 272 patients with stable coronary artery disease and received PCI were consecutively enrolled from January to December 2013 and followed up for 2 years. Patients were divided into O group and non-O group according to their ABO groups. Multivariable COX regression was used to evaluated the relationship between ABO blood groups and prognosis of CAD. The endpoints included all-cause death, cardiac death, myocardial infarction(MI), revascularization, and stroke.

There were 1 302 patients in O group and 2 970 patients in non-O group. ABO blood group was not associated with age, sex and blood pressure (P>0.05). The comorbidity rate of hypertension, diabetes mellitus, smoking, family history, previous MI, previous cerebrovascular disease, previous PCI and left ventricular ejection fraction were similar between the two groups (P>0.05). Total cholesterol, low density lipid cholesterol level were significantly higher in non-O group compared with O group [(4.2±1.1)mmol/L vs (4.1±1.1)mmol/L, P=0.027; (2.5±0.9)mmol/L vs (2.4±0.9) mmol/L, P=0.025], while high density lipid cholesterol level was significantly lower[(1.04±0.26) mmol/L vs (1.06±0.28) mmol/L, P=0.035]. As to angiographic results, non-O blood group was not related to the severity of coronary atherosclerosis assessed by SYNTAX score(P=0.277). More cardiac death occurred in non-O group compared with that in O group [21 (0.7%) vs 2(0.1%)] during 2-year follow-up. After adjusted for confounding factors, multivariable COX regression revealed that non-O blood type was not associated with increased cardiac death [HR (95%CI)=7.30(0.97-55.09), P=0.054].

Non-O blood group is associated with 2-year cardiac death in patients with stable coronary artery disease who received PCI, but it is not an independent risk factor for cardiac death.

To explore the value of Clermont score in the detection of intestinal mucosal ulcer in Crohn's disease (CD).

From May 2015 to August 2017, 45 patients (28 males and 17 females; median age was 25 years) were confirmed to have ileocolic CD by endoscopic and pathological examinations at Nanjing General Hospital. All patients underwent MRE and DWI examinations. Based on the appearance of intestinal mucosa endoscopically, intestine segments from 45 patients were divided into three groups, namely, no ulceration group (NU), superficial ulceration group (SU), and deep ulceration group (DU). Several factors contribute to Clermont score calculation. These included the measurement of intestinal wall thickness using MRE, evaluation of intestinal wall edema and ulceration by MRE, DWI performance and ADC value of each segment. One-way ANOVA was utilized to compare the Clermont scores and ADC values of the intestine segments among the three groups. The efficacy of the Clermont scores and ADC values in evaluating intestinal mucosal ulcer in CD was verified using ROC analysis.

A total of 137 intestine segments were included in the study with 30 cases in NU, 37 cases in SU, and 70 cases in DU.DU had the highest Clermont score (22.5±4.5),following were SU(15.8±3.5) and NU(10.2±1.3)(F=179.935,P<0.01).The ADC values of DU ((1.34±0.17)×10^-3mm²/s) was lower than NU ((2.07±0.52)×10^-3mm²/s) and SU ((1.52±0.23)×10^-3 mm²/s) (F=83.822,P<0.01).The AUCs of using Clermont score and ADC value in differentiating deep ulcerations were 0.887 and 0.733, respectively.

Either Clermont score or ADC value can be used to evaluate mucosal ulcer in CD. Clermont score demonstrates a better efficacy than ADC value in detecting deep ulcerations.

To investigate the clinical, pathological and CT features associated with the effective mutation of epidermal growth factor receptor (EGFR) in multiple primary lung cancers (MPLCs) , and to determine the target population of EGFR mutations tests.

A total of 558 nodules from 232 patients with MPLCs who underwent surgery in the Cancer Hospital of Chinese Academy of Medical Sciences from August 2017 to December 2017 were selected. Two hundreds and sixteen nodules were detected by DNA direct sequencing. Chi-square test and Mann-Whitney U test were used to compare the clinical, pathological and CT features of 216 nodules in the EGFR effective mutation group and the non-effective mutation group. Logistic regression analysis was used to explore the independent risk factors of EGFR mutation. The cut off value was determined using the receiver operating characteristic(ROC) curve. Of 232 cases 558 nodules of surgically resected MPLCs, EGFR mutation of 216 nodules was determined by direct DNA sequencing.

There were 58 males and 174 females with MPLCs(male︰female=1︰3). There were 117 cases of age ≥59 years old and 115 cases of age <59 years old. There were 192 non-smokers, accounting for 82.8% of all patients. There were 2-7 nodules in the patient′s lungs, of which 170 patients had two nodules in the lungs, 44 patients had 3 nodules, and another patient had 7 nodules. Among them, 216 nodules were detected by EGFR gene, 136 were effective mutations, and 80 were non-effective mutations (including wild type and null mutation). EGFR effective mutation group and non-effective mutation group were statistically significant in lung adenocarcinoma patients with different gender, age, smoking history, histological type, and differentiation degree ( P=0.006, 0.002, 0.002, 0.015, 0.025).Among them, the effective mutation group were 107 females, 85 cases≥ 59 years old, 117 cases with no smoking history, 68 acinar-based, 89 moderate differentiation. In the count data, 127 nodule edges were lobed, and only 9 nodule edges were smooth. Among the measurement data, the GGO CT value was approximately (-459±147) HU in the EGFR mutation group, with statistical difference (P=0.037). The GGO diameter was approximately (11±9)mm,P=0.279.Multivariate Logistic regression analysis showed that GGO diameter (OR=0.873, 95%CI: 0.780-0.997; P=0.018) and smooth margins (OR=0.183,95%CI: 0.041-0.824; P=0.027) were independent protective factors of effective mutations of EGFR.

In MPLCs, effective EGFR mutation is more common, and associated with elder female, age≥59 years, non-smoking, GGO attenuation <-548 HU, moderately differentiated, predominant invasive papillary adenocarcinoma. Patients with MPLCs and these risk factors may be encouraged to have postoperative EGFR molecular test.

To investigate the accuracy of Gaze-face-arm-speech-time (G-FAST) score in the early diagnosis of acute anterior circulation stroke patients with large artery occlusion.

A retrospective study was conducted to investigate the anterior circulation infarction (ACI) cases with complete vascular imaging data within 6 hours of onset in the Department of Neurology of Beijing Shijitan Hospital, Capital Medical University from May 2010 to April 2018. According to the results of digital subtraction angiography (DSA) or computed tomography angiography (CTA), the patients were divided into two groups: large artery occlusion group and non-large artery occlusion group. Accuracy of G-FAST score in predicting acute large artery occlusive stroke was analyzed by area under receiver operating characteristic curve (AUC). The predictive value of G-FAST score, National Institutes of Health Stroke Scale (NIHSS) score and Alberta stroke early CT score (ASPECTS) in predicting large artery occlusion was compared.

A total of 138 patients with acute anterior circulation ischemic stroke were included in the study, and 82 of them had large artery occlusion (59.4%). Univariate analysis showed that baseline NIHSS score (12.0 vs 8.9, P=0.000) and G-FAST score (3.1 vs 2.2, P=0.000) were significantly higher in patients with large artery occlusion than those without large artery occlusion, and ASPECTS was significantly lower than patients without large artery occlusion (7.4 vs 8.2, P=0.001). The results from ROC showed that G-FAST, NIHSS and ASPECTS were with the AUC of 0.781, 0.733 and 0.664, respectively. G-FAST score had the highest accuracy in predicting the anterior circulation arterial occlusion. The optimal threshold of G-FAST score was 2.5, with a sensitivity of 79.3% and a specificity of 64.3%. Further univariate analysis showed that percentage of large anterior vessel occlusion (LAVO) in G-FAST (≥3) group was significantly different from that in G-FAST (≤ 2) group [76.5%(65/85)∶32.1%(17/53), P=0.000].

G-FAST score has predictive value for acute anterior circulation arterial occlusive stroke. Endovascular treatment may need to consider in patients with high G-FAST score as early as possible when conditions permit.

To summarize the clinical outcome of abnormal prokaryotic embryos [0PN (non-pronuclear), 1PN (one-pronuclear)] transfer for reference in clinical work.

To collect the clinical data of infertility patients who received in vitro fertilization/intracytoplasmic sperm microinjection-embryo transfer from January 1, 2013 to December 31, 2018 at the reproductive center of Beijing Chaoyang Hospital Affiliated to Capital Medical University, and to retrospectively analyze the pregnancy outcome of the cases of embryo transfer from 0PN and 1PN sources.

Forty-seven patients were transplanted non-2PN-derived embryos during 50 cycles. There were 18 cycles which only 0PN embryos were transplanted, and the clinical pregnancy rate was 50.0%. There were 17 cycles which only 1PN embryos were transplanted, and the clinical pregnancy rate was 29.4% (compared with 0PN, the difference was not statistically significant, P>0.05). No abnormalities were found in the delivered newborns.

In the absence of 2PN-derived embryos, patients can be transplanted blastocysts from 0PN and 1PN sources. Acceptable clinical pregnancy outcomes can be achieved, and the clinical outcomes of 0PN may be better than 1PN.

To screen the differentially expressed genes, functional enrichment and related signaling pathways in glioma by bioinformatics analysis.

Microarray data of glioma related gene expression profiles were selected in GEO database, and differentially expressed genes in glioma patients and normal brain tissues were screened by R statistical software of lima package. Functional enrichment of differentially expressed genes (GO and KEGG) was performed. The protein-protein interaction database (STRING) was used to analyze the interaction between the screened differentially expressed genes and the related signaling pathways.

Two gene expression profiles, GSE15824 and GSE66354, were selected for analysis, and 158 genes with differential expression more than 2 times and P<0.05 were screened. Molecular function (MF) of 158 differentially expressed genes was integrin binding, cell adhesion molecule binding, calcium binding and AMPA glutamate receptor activity. Cell component localization (CC) was located in cell membrane, neuron cell body, axon of nerve cell and so on, while biological process (BP) was mainly cell adhesion and nervous system. Development, cell proliferation, GTPase activity, apoptosis and angiogenesis; KEGG signaling pathways were mainly cAMP signaling pathway, purine metabolism pathway, MAPK signaling pathway and cGMP-PKG signaling pathway. There were 177 interaction connections in 158 differential expression gene-protein interaction networks, with an average interaction of 2.39 between each node and an aggregation coefficient of 0.37. Cytohubb screened the key genes (hub genes) in the signaling pathway. The results indicated that SLC6A1,SLC1A2,BDNF,GAP43,NRXN1,GAD1,OLIG2, PLP1,S100B and GRIA3 were the key genes in the signaling pathway of the interacting protein network. All the 10 key genes were related to the prognosis of patients (P<0.05).

There are differentially expressed genes profile in glioma tissues and normal tissues. SLC6A1, SLC1A2, BDNF, GAP43, NRXN1, GAD1, OLIG2, PLP1, S100B and GRIA3 are key genes for glioma development and are related to the prognosis of patients.

To investigate the value of the area of levator hiatusin diagnosis of uterine prolapse.

From September 2017 to December 2018, 80 patients diagnosed with uterine prolapse by Department of Gynecology, Xiangya Hospital of Central South University were selected as the case group, and 80 cases of normal women in the same period were selected as the control group. All subjects in both groups were examined by transperineal three-dimensional ultrasound. The anteroposterior and transverse diameters and the area of levator hiatus were measured at rest and during maximum Valsalva maneuver respectively. The ROC curve was drawn to determine the cut-off value of area of levator hiatus in diagnosis of uterine prolapse and to evaluate its diagnostic value.

At rest and during maximum Valsalva maneuver, the anteroposterior and transverse diameters and the area of levator hiatus in study group were larger than those in control group, the difference was statistically significant (P<0.05). During maximum Valsalva maneuver, the above values in both groups were greater than those of the same group at rest state, but there was no significant difference between the two groups (P>0.05). During maximum Valsalva maneuver, the best cut-off value of area of levator hiatus for the diagnosis of uterine prolapse was 22.09 cm², the area under curve was 0.893. The sensitivity, specificity and accuracy were 90.0%, 95.0% and 92.5%, respectively.

Transperineal three-dimensional ultrasound can evaluate the morphological changes of levator hiatus in patients with uterine prolapse, and the area of levator hiatus has high diagnostic value for uterine prolapse.

Mesenteric heterotopic ossification (HMO) is clinically rare and is a pseudotumor-like reactive lesion. This patient, a 60-year-old male, complained of repeated abdominal distension and abdominal pain for 30 years, accompanied by cessation of anal exhaustion and defecation for 2 months. CT showed a strip-like dense shadow of the mesentery of the right middle and lower abdominal small intestine, and the CT bone window showed a dense edge, a slightly lower density bone marrow-like area in the center, and the surrounding intestine was aggregated into a mass. Pathological diagnosis: HMO.