Volume 95  ·  Issue 20  ·  2015

Acute gastrointestinal hemorrhage is one of the common medical emergencies in clinic. Implementing rapid and effective diagnosis and treatment for patients at the first time is the key to saving patients' lives and improving their prognosis. Therefore, in clinical work, it is a clinical skill that every physician should master to quickly judge the cause of gastrointestinal bleeding, accurately assess the severity of the disease, and take appropriate measures for different situations.

stress ulcer (SU) refers to acute gastrointestinal mucosal erosion, ulcer and other lesions that occur in various stressful states such as severe trauma, critical illness or severe mental illness. In severe cases, it can be complicated by gastrointestinal bleeding or even perforation, which can aggravate and worsen the original disease and increase the mortality rate. Therefore, prevention of SU is a link that cannot be ignored in the treatment of critically ill patients. SU can be manifested as acute gastric mucosal lesions, acute erosive gastritis, acute hemorrhagic gastritis, peptic ulcer, etc. under endoscopy.

To investigate the relationship between the Trp64Arg polymorphism of β3-adrenergic receptor (ADRB3) gene and obesity and the levels of blood lipids in the Northern Han Chinese population.

A total of 1 602 subjects in northern Han Chinese were recruited in this study, including 995 males and 607 females. Genotyping was performed using the TaqMan assay to identify the Trp64Arg polymorphisms of the ADRB3. The relationship between the polymorphism and obesity and blood lipids was analyzed.

The genotype distribution for Trp64Arg polymorphism was in conformity with the Hardy-Weinberg equilibrium (HWE) in the recruited population (χ²=0.043, P=0.087). The frequency of Arg64 allele was 15.82%. In the overall analysis, no significant association was showed between the Trp64Arg polymorphism and the Body Mass Index (BMI) or the levels of blood lipids. Subgroup analysis was performed by hypertension.In the hypertensive subgroup, the results showed significant association between the polymorphism and the levels of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and the ratio of TC and high-density lipoprotein cholesterol (TC/HDL-C)(TC: (5.27±1.04)mmol/L vs (5.10±1.02)mmol/L, t=2.334, P=0.02; LDL-C: (3.42±0.88)mmol/L vs (3.27±0.87)mmol/L, t=2.067, P=0.039; TC/HDL: 4.81±1.31 vs 4.57±1.25, t=2.563, P=0.011). Stepwise multiple regression analysis showed that there were significant associations between the Trp64Arg polymorphism and the levels of blood lipids. The polymorphism might affect 3.2% variances of the level of TC (P=0.030, R²=0.032), 2.5% variances of the level of LDL-C (P=0.030, R²=0.025), 3.4% variances of the TC/HDL-C ratio (P=0.001, R²=0.034), respectively. In the normatensive subgroup, there was no significant association between the polymorphism and the levels of lipids. No significant association was observed between the polymorphism and BMI either in the hypertension group or in the normotension group.

The Trp64Arg polymorphism in ADRB3 gene may be associated with the levels of blood lipids of the Chinese Han patients with essential hypertension. The hypertensive patients with the Trp64Trp genotype may be liable to dyslipidemia.

To observe the changes of islet function in ZnT8A positive and IA-2A positive patients so as to provide rationales for early intervention of LADA patients.

A total of 69 outpatients and inpatients with newly diagnosed LADA testing for GADA, IA-2A and ZnT8A by radioligand binding assay (RBA) were enrolled from 2005 to 2010, including 13 with double positive for GADA and ZnT8A (GADA+ ZnT8A double positive group), 14 with double positive for GADA and IA-2A (GADA+ IA-2A double positive group) and 42 with positive for GADA alone (GADA positive group), 40 type 2 diabetes mellitus (T2DM) patients negative for the above three autoantibodies were selected as control subjects (T2DM group). The baseline clinical data of all groups were compared and all patients were followed annually for 3 years. Fasting plasma glucose (FBG), HbA1c, fasting C-peptide (FCP), C-peptide 2 h after meal (2 h CP), triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C) were detected and compared among the above groups during the follow-ups.

Compared to GADA positive group and T2DM group, the GADA+ IA-2A double positive group had higher level of baseline FBG and HbA1c((10.5±3.6) mmol/L and (9.3±2.6)% vs (9.2±2.0) mmol/L and(7.4±2.0)%, P=0.017, 0.011) and had lower level of baseline FCP (415(68-934) vs 538(224-1 146)and 643(163-1 658)pmol/L, P=0.015, 0.003). During the follow-ups, FCP decreased year-on-year in GADA+ ZnT8A double positive, GADA+ IA-2A double positive and GADA positive groups. The annual average rate of decrease in FCP was more rapid in GADA+ ZnT8A double positive and GADA+ IA-2A double positive groups than those in GADA positive alone and T2DM groups (all P<0.01).

The islet functions of newly diagnosed LADA patients with GADA and IA-2A positive are much worse. And emergence of ZnT8A and IA-2A may accelerate the failure of islet function with diabetic progress in LADA patients.

To compare the efficacies of pulsatile gonadotropin-releasing hormone (GnRH) versus human chorionic gonadotropin/human menopausal gonadotropin (HCG/HMG) for spermatogenesis in male idiopathic hypogonadotropic hypogonadism (IHH).

For this retrospective study, a total of 92 male IHH outpatients from May 2010 to October 2014 were recruited and categorized into GnRH (n=40) and HCG/HMG (n=52) groups. Each subject selected one specific therapy voluntarily. The gonadotropin levels were measured in the first week and monthly post-treatment in GnRH group. And serum total testosterone (TT), testicular volume (TV) and rate of spermatogenesis were observed monthly post-treatment in two groups. Spermatogenesis, TT and TV were compared between two groups.

All IHH patients were treated for over 3 months. The median follow-up periods in GnRH and HCG/HMG groups was 8.2 (3.0-18.4) and 9.2 (3.0-18.6) months respectively (P=0.413). In GnRH group, LH ((0.5±0.6) vs (3.4±2.4)U/L, P<0.01) and FSH ((1.3±1.1) vs (5.8±3.8)U/L,P<0.01) increased after 1-week treatment. In GnRH group, at the end of follow-up, TT ((1.0±1.0) vs (7.4±5.2)nmol/L,P<0.01) and TV ((2.3±1.5) vs (8.1±4.0) ml,P<0.01) significantly increased compared to baseline. In HCG/HMG group, TT ((0.8±0.6) vs (14.4±8.0) nmol/L,P<0.01) and TV ((2.3±2.1) vs (7.6±4.2)ml,P<0.01) significantly increased after therapy. The success rate of spermatogenesis was 50.0% (20/40) in GnRH group versus 28.8% (15/52) in HCG/HMG group (P=0.038). GnRH group required a shorter treatment time for initial sperm appearance than HCG/HMG group ((6.5±3.1) vs (10.8±3.7) months, P=0.001).

Pulsatile GnRH requires a shorter time for initiation of spermatogenesis than gonadotropin therapy in IHH male patients.

To evaluate the outcomes of intravenous glucocorticoid therapy with or without orbital radiotherapy for moderate-to-severe Graves′ ophthalmopathy.

A total of 83 patients with moderate-to-severe Graves′ ophthalmopathy were retrospectively analyzed. Intravenous glucocorticoid was given to patients for 3 consecutive days every four weeks in 1-3 circles. The regimens included intravenous glucocorticoid alone (n=36) and intravenous glucocorticoid plus orbital radiotherapy (n=47). Photophobia, lacrimation, eye pain, soft tissue congestion, edema, exophthalmos and diplopia were compared before and after treatment. The symptoms and efficacies were compared between two groups.

During a 12-week follow-up, clinical activity score (CAS), exophthalmos and diplopia all improved after treatment (P<0.05). However, the changes of CAS, exophthalmos or diplopia showed no inter-group differences (P>0.05). The overall clinical response was 50% in glucocorticoid group and 63.8% in glucocorticoid plus orbital radiotherapy group. And there was no statistical difference (P>0.05).

Moderate-to-severe Graves′ ophthalmopathy may be relieved by pulsed intravenous glucocorticoid with or without orbital radiotherapy. There is no inter-group difference in short-term efficacies.

To explore the associations of interleukin-23 (IL-23) receptor gene polymorphisms, susceptibility to pulmonary tuberculosis and drug-resistant pulmonary tuberculosis (TB).

For this paired case-control study, 13 single nucleotide polymorphisms (SNPs) in IL-23R gene were genotyped by multiplex SNaPshot with samples from 250 pairs of culture positive pulmonary TB patients and healthy controls. And the relationship between genotype, allele, haplotype and susceptibility to pulmonary tuberculosis was analyzed.

Two SNPs of IL-23R gene (rs10889677 and rs7518660) (OR=1.53, 95%CI:1.01-2.31; OR=6.25, 95%CI:3.85-10.00) were significantly associated with susceptibility to pulmonary TB and one SNP (rs11465802) (OR=2.90; 95%CI:1.69-4.98) was associated with drug-resistant pulmonary TB in the Chinese Uygur population.

Two SNPs of IL-23R gene, rs10889677 and rs7518660, may be the risk factors of pulmonary tuberculosis. And rs11465802 may be a risk factor of drug-resistant pulmonary tuberculosis in Chinese Uygur population.

To analyse population pharmacokinetic (PPK) parameter values of ciprofloxacin in Chinese elderly patients with lower respiratory tract infection.

Hospitalized in Respiratory Intensive Care Unit (RICU) due to severe lower respiratory tract infection and at high risks of Pseudomonas aeruginosa. 43 consecutive elderly patients received an intravenous infusion of ciprofloxacin at a dose of 200/400 mg every 12/24 h empirically. Plasma samples were drawn from Day 4 and at 1, 2, 3, 4, 6, 8 and 12 h after infusion. The serum concentrations of ciprofloxacin were determined by high-performance liquid chromatography (HPLC). Ciprofloxacin population pharmacokinetic analysis was conducted by the NONMEM 7.3.0 software.

Forty patients aged (78±9) years completed the study. A total of 210 serum concentrations were detected. The mean values of clearance and volume of distribution (V₁) were 17.8 L/h and 49.8 L respectively. The serum creatinine level influenced ciprofloxacin according to the equation: Clearance =17.8×[1-0.001 3×(serum creatinine-67)] ×e^η1i×0.659ⁿ(at a dose of 200 mg, n=1, at a dose of 400 mg, n=0; ηi for inter individual random variation). The values of clearance and V ₁were multiplied by 0.659 at a dose of 200 mg. At the doses of 200 and 400 mg, the maximum steady-state concentrations (C_max) were (4.2±1.1) and (5.3±1.21) mg/L, the minimum steady-state concentrations (C_min) (1.1±1.1) and (0.8±0.4) mg/L and the area under concentration-time curve measured in steady-state up to 24 h after dosing (AUC_{0-24 h}) were (45.5±28.1) and (47.2±11.3) mg·h·L^-1respectively.

The clearance of ciprofloxacin in elderly patients significantly decreases as compared with adult patients. Ciprofloxacin PPK model shows a significant influence of serum creatinine level on ciprofloxacin clearance. A single dose of 400 mg may increase serum drug concentration, but causes no in vivodrug retention.

To observe the vary of postoperation cognitive function and inflammatory mediators CRP in elderly patients with fracture after using ulinastatin.

Forty-eight patients over 65 years old patients with hip fracture were randomly divided into two groups: ulinastatin group(n=21)and control group(n=27). All patients underwent combined spinal-epidural anesthesia. The patients in ulinastatin group received intravenous injection of ulinastatin with a dose of 5 000 U/kg before skin incision and at the moment of the end of operation. Cognitive function was assessed by Mini-Mental State Examination(MMSE)at the day of the operation(T₀), 1 (T₁), 3 (T₂)and 7 (T₃) days after opreation. The incidence of POCD was calculated. Blood samples were taken at the day of the operation and 3 days after operation for determination of CRP.

Compared with control group(23.8±0.8, 24.3±0.7), the MMSE scores were significantly increased at T₁, T₂in ulinastatin group(26.0±0.7, 26.6±0.6) (t₁=2.161, t₂=2.431, P<0.05). There were no differences at T₀, T₃(t₀=0.472, t₃=1.585, P>0.05). The respectively incidence of POCD of ulinastatin group and control group were 40.7% and 4.8%, and that of ulinastatin group was significantly lower than control group(χ²=8.155, P<0.01). Serum CRP concentration of ulinastatin group after operation was(64.46±9.86)mg/L, significantly lower than control group(123.63±7.17)mg/L (t=-4.974, P<0.05).

Ulinastatin can reduce the incidence of POCD in the elderly patients with fracture, which may be related to the anti-inflammatory effect of ulinastatin.

To analyze the characteristics and trends of gastrointestinal mucosal injury for age ≥45 years male health care patients during gastro-endoscopic follow-up.

Endoscopic reports of age ≥45 years male health care patients with long-term aspirin undergoing gastroscopy from October 1999 to April 2014 were retrospectively reviewed. The proportion of different lesions, the distribution at different anatomic sites, and the trends of the number of gastrointestinal mucosal injury in different follow up years were analyzed.

A total of 2 281 endoscopic reports of 259 health care cases aged ≥45 years with aspirin used 3 years at least were collected. After the initial gastroscopy screening, there are 239, 103 and 20 cases followed up to the 5^th,10^thand 15^thyears. The patients were between 45-91 years of age, their mean age was 67 years.The mean followed-up time was 5 years. In the follow-up process each patient had 8 gastroscopies performed. A total of 4 442 lesions were detected and the mean number was 2 per person for once. The number of gastrointestinal mucosal injuries were different.The number of the gastrointestinal mucosal injury of the 1^styear was higher than that of initial gastro-endoscopy (P<0.05). The numbers of the gastrointestinal mucosal injury of the 2^se-5^thyears during the follow up period were lower than those of initial gastro-endoscopy (all P<0.05). The numbers of three different lesions of gastrointestinal mucosal injury were different (P<0.05). The number of erosion was more than petechia and ulcer (bothP<0.05). The Modified Lanza′s Score (MDS) were in descending trend during the follow up period. The numbers of gastrointestinal mucosal injury in different anatomic sites were different in the same follow-up years(allP<0.05).

The numbers and degree of gastrointestinal mucosal injury for middle aged and aged health care patients with long-term aspirin used during gastro-endoscopic follow-up are in descending trend, however, there is a transient increasing in number of gastrointestinal mucosal injury in the first two years since the aspirin used. The common lesion in gastrointestinal mucosal injury is erosions.Gastric antrum and body are vulnerable anatomical parts during follow-up.

The program of continuous quality improvement in clinical laboratory processes for complete blood count (CBC) was launched via the platform of Beijing Children′s Hospital Group in order to improve the quality of pediatric clinical laboratories.

Fifteen children′s hospitals of Beijing Children′s Hospital group were investigated using the method of Chinese adapted continuous quality improvement with PDCA (Plan-Do-Check-Action). The questionnaire survey and inter-laboratory comparison was conducted to find the existing problems, to analyze reasons, to set forth quality targets and to put them into practice. Then, targeted training was conducted to 15 children′s hospitals and the second questionnaire survey, self examinations by the clinical laboratories was performed. At the same time, the Group′s online internal quality control platform was established. Overall effects of the program were evaluated so that lay a foundation for the next stage of PDCA.

Both quality of control system documents and CBC internal quality control scheme for all of clinical laboratories were improved through this program. In addition, standardization of performance verification was also improved, especially with the comparable verification rate of precision and internal laboratory results up to 100%. In terms of instrument calibration and mandatory diagnostic rates, only three out of the 15 hospitals (20%) failed to pass muster in 2014 from 46.67% (seven out of the 15 hospitals) in 2013. The abnormal data of intraday precision variance coefficients of the five CBC indicator parameters (WBC, RBC, Hb, Plt and Hct) of all the 15 laboratories accounted for 1.2% (2/165) in 2014, a marked decrease from 9.6% (14/145) in 2013. While the number of the hospitals using only one horizontal quality control object for daily quality control has dropped to three from five. The 15 hospitals organized a total of 263 times of training in 2014 from 101 times in 2013, up 160%.

The quality improvement program for the clinical laboratories launched via the Hospital Group platform can promote the joint development of the pediatric clinical laboratory discipline of all the member hospitals with remarkable improvement results, and the experience is recommendable for further rollout.

To explore the quantity and function of regulatory B cells(Bregs)in peripheral blood (PB)of the patients with immune thrombocytopenia(ITP).

A total of 67 ITP patients (35 patients were newly diagnosed, 32 patients were in remission) were collected from November 2013 to October 2014 in our hospital. And 30 normal controls were enrolled in this study. Their PB Bregs (CD19⁺CD24^hiCD38^hicells) and related molecules interleukin-10(IL-10) in Bregs were detected by flow cytometry (FCM). Correlation between Bregs and clinical parameters of ITP patients was analysed.

The percentage of Bregs in CD19⁺cells of newly diagnosed ITP patients(3.09%±2.57%)was significantly lower than that of remitted ITP patients (7.78%±6.59%, P=0.002) and controls(5.42%±3.31%, P=0.003). The percentage of IL-10⁺cells in Bregs of newly diagnosed ITP patients (32.30%±12.71%)was significantly lower than that of remitted ITP patients(71.73%±18.08%, P=0.000)and controls(51.01%±17.08%, P=0.026). And the percentage of IL-10⁺cells in Bregs of remitted ITP patients was significantly higher than that of controls(P=0.026). The level of Bregs of newly diagnosed ITP patients had significantly positive correlation with myeloid dendritic cells(mDC)(r=0.644, P=0.003)and PB platelet count(r=0.327, P=0.006).

Bregs involved in the pathogenesis of ITP might be a potential therapeutic target of this disease.

To explore the role and underlying mechanisms of insulin-like growth factor Ⅰ(IGF-Ⅰ)/extracellular signal-regulated kinase (ERK) signaling pathway in lung fibrosis.

Alveolar epithelial cell A549 was used. The expressions of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) in A549 culture media stimulated by IGF-Ⅰwere determined by enzyme-linked immunosorbent assay (ELISA). ERK inhibitor U0126 was used. And there were three groups of control, IGF-Ⅰstimulation and RK inhibitor plus IGF-Ⅰstimulation. The activity of ERK in three groups was measured by Western blot. The mRNA level and protein concentration of MMP-2 and MMP-9 in three groups were examined by quantitative real-time-polymerase chain reaction (qRT-PCR) and ELISA.

The protein concentrations of MMP-2 and MMP-9 in cell culture media increased in 50, 100 ng/ml IGF-Ⅰgroups as compared with 0 ng/ml IGF-Ⅰgroup ((18.30±0.11), (21.80±0.09) vs (13.52±0.19) ng/ml and (0.34±0.01), (0.39±0.01) vs (0.25±0.01) ng/ml, P<0.001). And the protein concentrations of MMP-2 and MMP-9 in 100 ng/ml IGF-Ⅰ group increased versus 50 ng/ml IGF-Ⅰ group (P<0.01). IGF-Ⅰ stimulation group increased the expression of p-ERK1/2 versus control group (0.40±0.01 vs 0.23±0.02,P<0.05) while ERK inhibitor plus IGF-Ⅰ stimulation group decreased the expression of p-ERK1/2 versus IGF-Ⅰ stimulation group (0.14±0.03 vs 0.40±0.01,P<0.01). ERK inhibitor plus IGF-Ⅰ stimulation group inhibited the mRNA levels of MMP-2 and MMP-9 versus IGF-Ⅰ stimulation group (0.88±0.03 vs 1.17±0.05 and 0.82±0.23 vs 1.81±0.27, bothP<0.05) and decreased the concentrations of MMP-2 and MMP-9 in culture media versus IGF-Ⅰ stimulation group ((21.70±0.32) vs (29.15±0.34) ng/ml and (0.22±0.01) vs (0.29±0.01) ng/ml, bothP<0.01).

IGF-Ⅰ induces the expressions of MMP-2 and MMP-9 in alveolar epithelial cell through ERK signaling pathway. And it is associated with the disruption of epithelial basement membrane and the development of lung fibrosis.

To explore the role of calcitonin gene-related peptide (CGRP) in the proliferation of vascular smooth muscle cells (VSMCs) and restenosis.

The high-expression CGRP lentivirus [Lenti-green fluorescent protein (GFP)-CGRP] was constructed. And mesenchymal stem cells (MSCs) were transfected with Lenti-GFP-CGRP, Lenti-GFP and phosphate buffer saline (PBS). Reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) were used to determine the CGRP gene and protein expression level of smooth muscle cells in each group respectively. Then MSCs were co-cultured with VSMCs. Experimental groups were MSCs^{CGRP+ /+}+ VSMCs, MSCs^CGRP-/-+ VSMCs and MSCs^PBS+ VSMCs groups. The method of methyl thiazolyl tetrazolium (MTT) was employed to detect the proliferation of smooth muscle cells. Sacculus damaged atherosclerotic carotid was prepared according to the previous study. MSCs were transfected with Lv-CGRP-EGFP and Lv-CGRP and then transplanted into rat model. At Day 7 post-transplantation, injured carotid artery was harvested to detect the C expression of GRP by Western blot and the expression of CD31 by immunofluorescence. At Day 28 post-transplantation, injured carotid artery was harvested to assess organization morphology by hematoxylin and eosin stain and detect the expression of proliferating cell nuclear antigen (PCNA) by immunohistochemical stain.

As compared with control and Lenti-GFP groups, the expressions of CGRP protein and CGRP mRNA increased at 72 h after transfecting with Lenti-GFP-CGRP (P<0.05). After 72-hour co-culturing with VSMCs, the proliferation ability of VSMCs was the lowest in MSCs^{CGRP+ /+}+ VSMCs group versus other three groups (P<0.05). At Day 7 post-transplantation, as compared with control and Lenti-GFP groups, the expression of CGRP proteins increased significantly in MSCs-CGRP group (P<0.05). A continuous expression of CD31 was found in damaged carotid intima in MSCs-CGRP group, but not in control group. At Day 28 post-transplantation, the area of intimal hyperplasia was smaller in MSCs-CGRP group than that in control and Lenti-GFP groups. Also the expression of PCNA decreased more in MSCs-CGRP group than control and Lenti-GFP groups (P<0.05).

CGRP protein and CGRP mRNA are expressed after CGRP transfection. And CGRP can suppress the proliferation of VSMCs and reduce intimal hyperplasia so as to facilitate a recovery of damaged endothelium.

A 51-year-old female was admitted to the hospital mainly for "anorexia, fatigue for 6 months and fever in the afternoon for 20 days". The patient developed anorexia, general fatigue and weight loss of approximately 4 kg without obvious trigger 6 months ago. Afternoon fever occurred 20 days ago, with a maximum body temperature of 39 ℃. There was no fear of cold, night sweats, no cough, expectoration and hemoptysis, no nausea, vomiting, no abdominal pain, abdominal distension and diarrhea, and the body temperature could drop to normal after symptomatic treatment. Abdominal B-ultrasound was performed in other hospitals to find multiple liver mass occupation, and multiple liver metastases were considered. An ultrasound-guided liver biopsy was performed in an external hospital, and the result was chronic granulomatous inflammation of the liver, showing caseous necrosis, and no acid-fast bacilli were found in acid-fast staining. Considering inflammatory lesions, neoplastic lesions were not excluded. 30 years of prior chronic viral hepatitis B (hepatitis B) history. Physical examination showed no obvious positive signs.

inflammatory bowel disease (IBD) is a chronic recurrent disease of the gastrointestinal tract, mainly including ulcerative colitis (UC) and Crohn's disease (CD). Epidemiological surveys show that the incidence of IBD in developing countries has increased significantly in the past 20 years. IBD can greatly affect the quality of life of patients and is related to the occurrence of intestinal malignant tumors. It has become one of the hotspots of modern gastrointestinal diseases. The etiology of IBD is unclear, and it is currently believed to be related to many factors such as genetics, immunity, infection and environment. In recent years, many studies have suggested that gastrointestinal microorganisms are related to the pathogenesis of IBD, but the specific pathogens are still unclear. Helicobacter pylori (H.pylori, HP) is a common gram-negative microaerobic bacterium belonging to the genus Helicobacter that has been in symbiosis with humans for more than 50 000 years. Many studies have confirmed that HP infection is closely related to a variety of digestive tract diseases^[1]。 In recent years, many studies have found that HP infection and inflammatory bowel disease are also related, but the conclusions of each study are not consistent. This paper reviews the research and progress of the relationship between HP infection and IBD, in order to have a more comprehensive understanding of the relationship between the two diseases, and to develop a new understanding of the nature and treatment of IBD.

On the occasion of the 100th anniversary of the founding of Chinese Medical Journal, the 100th Anniversary of the Founding of Chinese Medical Journal and the Summit Forum on High-tech Medical Development and Internet Medical Care, sponsored by the Editorial Committee of Chinese Medical Journal and Huashan Hospital affiliated to Fudan University and co-organized by Registration Network, was grandly held in Shanghai, the birthplace of Chinese Medical Journal, on March 28th, 2015. More than 350 experts and scholars from all over the country attended the event. Professor Chen Xiangmei of PLA General Hospital, Academician of Chinese Academy of Engineering, Professor Wang Chen, President of Sino-Japanese Hospital, Academician of Chinese Academy of Engineering, Professor Ge Junbo of Zhongshan Hospital affiliated to Fudan University, Academician of Chinese Academy of Sciences, and Professor Hejie, President of Cancer Hospital of Chinese Academy of Medical Sciences, Academician of Chinese Academy of Sciences, respectively wrote inscriptions and called to express warm congratulations on the convening of the meeting. Chen Xinshi, deputy editor-in-chief and director of the editorial department of Chinese Medical Journal, edited and reviewed, and Professor Zhang Qunhua of Huashan Hospital affiliated to Fudan University co-hosted the conference.