Volume 91  ·   Issue 47  ·  2011

osteonecrosis of the femoral head (ONFH) has a high disability rate, which has a great negative impact on the patients,family and social production.Aiming at this difficult problem in orthopedic medicine, scholars at home and abroad have done a lot of exploration work on this problem, but it has not been completely solved.As a mature and classical orthopedic treatment technique, joint replacement has achieved great success in the treatment of hip joint diseases.But is it suitable for artificial joint replacement for young and middle-aged ONFH patients with necrosis volume in 1/3~1/2? Many doctors suggest joint replacement surgery, but the long-term effect is not satisfactory [1]. We suggest head-preserving treatment for such patients, and try to delay or even avoid joint replacement treatment.

Objective To examine the basic biomechanics of locking plate fixation plus calcium phosphate bone cement (CPC) for unstable intertrochanteric fractures.Methods A total of 18 Chinese fresh femur specimens without pathological defects,fractures,malformations or neoplasms were collected.In accordance with the Evans classification of Ⅲ A type, the intertrochanteric fracture model was established.There were 18 femoral samples in each group.The experimental group received locking plate (LPFP) fixation plus CPC while the control group had a simple LPFP fixation.The mechanical tests were performed.Results Under different load-strain conditions,the results were as follows:600 kg · m · s-2 experimental group 98 +3.6 vs control group 127 +4.4 με; 3000 kg · m · s-2 experimental group 278 +23 vs control group 457 + 45 με.And under different load-displacement conditions,500 kg · m · s-2experimental group 0.16 +0.02 vs control group 0.23 +0.04 mm; 4000 kg · m · s-2 experimental group 2.79 ± 0.59 vs control group 5.05 + 0.68 mm.The torsional mechanical 0.1 (°/cm) properties:experimental group0.14+0.01 vs control group 0.23 +0.04 (kg · m · s-2 · m); 0.8(°/cm) experimental group 3.14 + 0.17 vs control group 2.57 ( kg · m · s -2 · m) + 0.08 ( kg · m · s- 2 · m) ( P ＜ 0.05 ).Conclusion The combined regimen of locking plate fixation and calcium phosphate cement offers remarkable advantages over simple locking plate fixation in its excellent anti-compression and anti-torsion performances in the treatment of unstable intertrochanteric fractures.With a reasonable design,it has a greater stability.

Objective To investigate the clinical outcome for the treatment of osteonecrosis of the femoral head (ONFH) in advanced stage by a short-stem prosthesis preserving femoral neck in total hip arthroplasty (THA).Methods From June 2008 to December 2009,9 hips in 8 patients with advanced stage of ONFH were treated by a short-stem preserving femoral neck in THA.The mean age was 24.1 years ( range:20 - 36 ).There were 3 patients (3 hips) with alcohol-induced ONFH and 5 patients (6 hips) with steroid-induced ONFH.According to the classification of Association Research Circulation Osseous (ARCO),7 hips were in stage Ⅲ-C and 2 hips in stage Ⅳ respectively.The clinical outcomes were evaluated according to the Harris evaluation score and radiographic analysis.Results All patients were followed up for a mean duration of 18.1 months (range:12 -30).The mean Harris hip score improved from preoperative (42.8 ± 8.6) points to(92.8 ± 6.1 ) points at the time of final follow-up.The outcomes were excellent in 7 hips and better in 2 hips.Neither osteolysis of mortar and femur nor loose component was found from radiological films.The pain of all hips disappeared and no complications occurred.Conclusion The short-term clinical outcome is satisfactory for treating young patients with advanced stage of ONFH by a shortstem prosthesis preserving femoral neck in THA.

Objective To explore the intermediate outcomes of third-generation alumina-on-alumina total hip arthroplasty (THA).Methods A consecutive series of 165 primary alumina-on-alumina total hip arthroplasties were performed in 135 patients.Three patients died and 13 patients lost follow-up so that a total of 119 patients ( 146 hips) were available for study.The mean patient age was 53.4 ± 11.0 years old ( range:26 - 79).Hydroxyapatite.(HA) -coated press-fit acetabular cups were used in all cases.At femoral side,123 cases were implanted with HA-coated uncemented stems and 23 cases implanted with high polished double taped cemented stems.All patients were evaluated clinically and radiographically after a minimal follow-up duration of 60 months.Results The preoperative mean Harris hip score of 49.6 ± 7.9 points improved to 91.7 ±3.0 points at the last follow-up ( P ＜0.05 ).The 5-year survival for any reason lead to revision was 96.6％.Five hips were under revision,1 for acetabular shell loosening and migration,1 for Vancouver B2 periprosthetic fracture,1 for fracture of pure alumina liner and 2 for infections.All other prostheses demonstrated no radiographic evidence of loosening.No periprosthetic osteolysis was found.There were 2 cases of dislocations and 2 patients presented postoperatively with “squeaking-like” hips.Conclusion The clinical and radiographic outcomes after primary THA with third-generation ceramic-on-ceramic bearing surfaces are favorable after a minimal follow-up duration of 5 years.The modified alumina-on-alumina bearing implants offer a better option for younger and active patients.

Objective To explore the anti-reflux effects of esophagogastric anastomoses by reverse stapling with Nissen fundoplication (RSNF).Methods A total of 103 cases of lower esophageal carcinomas and esophagogastric junction carcinomas undergone reverse stapled anastomoses with Nissen fundoplication between July 2009 and December 2010 at our hospital were retrospectively reviewed.And fistula,stricture and gastroesophageal reflux were compared with those of 130 traditional cases.Results RSNF required more time than the traditional group ( 16.42 vs 14.02 min,P ＜ 0.001 ).No fistula was found in neither group.There were 13 complications in RSNF and 23 in the traditional group (x2 =0.44,P =0.58).The rate of stricture was 4.8％ (5/103) in RSNF and 6.9％ (9/130) in the traditional group (x2 =0.44,P =0.58).There were 16 reflux esophagitis ( 15.5％ ) in RSNF and 29 in the traditional group (22.3％ ) (x2 =2.57,P=0.46). The post-RSNF reflux symptoms were more severe than the traditional group (31.1％ vs 49.2％,x2 =7.825,P =0.007,score of GerdQ ＞ 8 ).Conclusion Reverse stapling with Nissen fundoplication may be conveniently performed with definite anti-reflux effects for esophagogastric anastomoses.

Objective To preliminarily explore the clinical characteristics and surgical outcomes of intracranial aneurysm.Methods The data of 3322 cases of intracranial aneurysm from January 1955 to July 2009 were collected at our hospital for analysis.The clinical characteristics and surgical outcomes of patients with intracranial aneurysm were assessed.The surgical patients were divided into 2 groups by the date of admission:group A and group B.Such clinical characteristics as age,gender and Hunt-Hess grade at admission and first presentations were analyzed.The surgical outcomes were assessed by comparing the surgical complications between two groups.Results Intracranial aneurysms were commonly diagnosed between 38 and 54 years old.Hemorrhage ( 84.89％ ),mass effect ( 8.13％ ) and non-hemorrhagic headache (5.18％ ) were the three initial common presentations and the male-to-female ratio was 0.875∶ 1.There was no significant statistical difference in age distribution and gender ratio between two groups.According to the Hunt-Hess grade,the percentage of grades Ⅱ,Ⅲ and Ⅳ was lower in group B while the percentage of grades 0 and Ⅰ higher than group A.No significant difference was found in the overall incidence of major post-operative complications between two groups ( P ＞ 0.05 ).Conclusion The major presentations of intracranial aneurysm are hemorrhage, mass effect and non-hemorrhagic headache.And the Hunt-Hess grades Ⅰ and Ⅱ patients account for a majority of aneurysms.Three most common locations of aneurysm are ICA-PCoA (internal carotid artery-posterior communicating artery ),ACoA ( anterior communicating artery) and MCA ( middle cerebral artery).Giant aneurysm should be treated in the light of concrete conditions by choosing such a surgical approach as bypass.

Objective To explore the effects of donepezil on the activities of platelet α and β secretases in Alzheimer's disease (AD) patients.Methods During the period of 2007 -2010,a total of 76AD patients received either regular treatment alone or in combination with donepezil (5 mg/d) for a 12-week period.And their effects on ADAS-Cog (Alzheimer's disease assessment scale-cognitive subscale) total and ADL (activity of daily living) scores were measured.The effects of donepezil on α and β secretase activities and sAPPα (soluble amyloid precursor protein α) secretion in AD patients and non-demented patients were detected by fluorescence and Western blot respectively.Results Mter the donepezil treatment,the ADASCog scores of the treatment group decreased versus the control [ ( 5.3 ± 4.4 ) vs ( 1.7 ± 1.6 ) ].And the differences were statistically significant ( P ＜ 0.01 ).And the ADL scores of the treatment group decreased versus the control [ (41 ±7) vs.(48 ±6) ].And the differences were statistically significant (P ＜0.05).As compared with that of pre-treatment (50 ± 6),the differences were statistically significant (P ＜0.05 ).The activity of α secretase increased markedly while that of β secretase decreased markedly versus the controls [(91％ ±9％) vs (64％ ±8％),P＜0.01; (119％ ±11％) vs (178％ ±17％),P＜0.01].Both had significant statistical differences with those of pre-treatment ( both P ＜ 0.01 ).As compared with the non-demented group ( 100％ ± 12％,P ＜0.001 ),the sAPPα contents of treatment and control groups were (64％ ±14％,P＜0.01) and (26％ ±8％,P＜0.001) respectively.Conclusion The administration of donepezil in AD patients improves cognitive functions and daily activities as indicated by the decreased ADAS-Cog total scores and ADL scores through the increased activity of α secretase and the decreased activity of β secretase.The clinical efficacy of donepezil may be attributed to its pharmacological effects on the regulation of platelet secretase activities.

Objective To explore the diagnostic valves of computed tomography perfusion imaging (CTP) in hyperacute cerebral infarction patients and examine the correlation of time period from symptom onset to examination and CTP parameters.Methods Non-enhancement CT and CTP were performed on 75 patients with acute cerebral infarction of internal carotid system within 8 hours of symptom onset at our department from January 2006 to May 2008.National Institute of Health Stroke Scale score (NIHSS),Barthel index (BI) and modified Rankin scale(mRS) were assessed at the same day,days 14 and 90 after stroke onset respectively for neurological function impairment,activity of daily living and extent of disability in prognosis.Results ( 1 ) All CTP parameters in ischemic region had no correlation with time period from symptom onset to examination (P ＞ 0.05 ).No significant differences were found between the patients with ＞3 hours and ＜ 3 hours after stroke onset in terms of the above parameters; (2) the areas of CBF ( cerebral blood flow) on ischemic region significantly correlated with NIHSS at the day of symptom onset (r =0.391,P＜0.001),Day 14 (r =0.564,P ＜0.001) and Day 90 (r =0.549,P ＜0.001) after symptom onset.CBV (cerebral blood volume) and TTP (time-to-peak) on ischemic region significantly correlated with the evaluation indices of predicting clinical outcomes at the day of symptom onset,Days 14 and 90 ( P ＜0.01).CBF,CBV,rCBF (regional CBF) and rCBV (regional CBV) correlated only with some of the clinical outcome scores.Conclusion Time period after symptom onset can not reflect the ischemic extent and volume of cerebral infarction.But CTP may evaluate the severity and prognosis of acute cerebral infarction patients.The area of abnormal perfusion is the most sensitive parameter.

Objective To explore the effects of propofol target controlled infusion (TCI) plus a low concentration of sevoflurane inhalation induction for the removal of tracheobronchial foreign body in children.Methods After the approval of the hospital ethics committee,a total of 90 patients,aged 9 - 36months old and weighted 8 - 17 kg,were randomly divided into 3 groups:group A,group B and group C.Propofol TCI plus a low concentration of sevoflurane inhalation induction was administered in group A while ketamine or fentanil plus propofol TCI in group B or C respectively.Effects of anesthesia,complications and recovery durations were observed.Results The incidence of severe breathholding and bucking during inserting bronchoscope was 1 case in group A,7 in group B and 5 in group C.There were significant differences between groups A and B( P ＜0.05 ).The minimal intra-operative SpO2 in group B or group C was lower than that in group A(P ＜0.01 ).The cases for intra-operative SpO2 ＜95％ in group B or group C were more than that in group A ( P ＜ 0.01 ).And the maximal target concentration of propofol was significantly higher than that in group A ( P ＜ 0.01 ).Ten cases in group B had laryngeal stridor and dyspnea during inspirations post-operatively and occurred more frequently than those in group A or C ( P ＜ 0.01 ).As compared with group A and C,post-operative staying lengths and recovery durations were significantly longer in those in group B( P ＜ 0.01 ).Conclusion Propofol TCI plus a low concentration of sevoflurane inhalation induction is both safe and practical for the controlled removal of tracheobronchial foreign body in children.

Objective To establish the serum proteomic models for the identification of premature delivery with inflammation or non-inflammation.Methods The laboring patients from 2008 to 2010 at our hospital were divided into 5 groups according to placental pathology,including inflammatory preterm group,non-inflammatory preterm group and blind test group ( n =50 each).The control group was normal fullterm.The preterm models with or without inflammation were established by the methods of SELDI-TOF-MS (matrix-assisted laser desorption/ionization-time of flight-mass spectrometry ) and bioinformatics.And statistical analysis was performed after a blind test.Then differential protein fingerprints were compared and analyzed.Results A total of 36 different proteins were harvested after a comparison of inflammatory preterm and control groups.The model was established by eight markers.The rates of specificity,sensitivity,positive predictive value and negative predictive value were 80.85％, 84.44％, 93.33％ and 46.67％respectively.Fifteen different proteins existed between non-inflammatory pretenn and control groups.There were 4 marker proteins.The rates of specificity,sensitivity,positive predictive value and negative predictive value were 75.68％,64.44％,80.00％ and 66.67％ respectively.Different marker proteins existed between the identification models of inflammatory preterm labor and inflammatory full-term. Conclusion Different serum proteomic models may be used for the diagnosis of preterm labor and the differentiation of preterm labor with or without inflammation.And different proteins are expressed during different stages of pregnancy with chorioamnionitis.

Objective To find the time window of normalization of tumor vasculature by endostar in tumor-bearing mice with ovarian cancer.Methods The nude murine model of ovarian cancer was established.The vasculature markers α-SMA (alpha-smooth muscle actin) and collagen Ⅳ covered tumor vessels and hypoxic zone following the treatment of endostar were monitored.According to the changes of microvascular morphology and tumor hypoxia zone,the time window was identified.Furthermore the treatment protocols of scheduling dosing of cisplatin plus endostar at different time points were designed.After treatment tumor volume,the parameters of microvascular density (MVD) and PCNA (proliferating cell nuclear antigen) were monitored to evaluate the effects of different protocols.Results At Days 4 and 6 posttreatment,more α-SMA and collagen Ⅳ covered vessels could be observed.The amount of microvasculature expressed α-SMA on days 4 compared with control was ( 15.3 ±5.2) vs (4.3 ±2.1 )/mm2 (P ＜0.01 ) ; at Day 6,the result was ( 16.4 ± 4.6) vs (6.6 ± 2.4 )/mm2 ( P ＜ 0.01 ).The expression of collagen Ⅳ had a similar change.And the numbers of microvasculature expressing collagen Ⅳ was ( 14.7 ± 4.3 ) vs (6.7 ±5.1 )/mm2 at Days 4 and 6 ( P ＜ 0.01 ) ; ( 18.4 ± 5.5 ) vs (7.1 ± 1.7 )/mm2 ( P ＜ 0.01 ).The expression of HIF-1α decreased in hypoxic area.In the rhES + DDP (d4 -6) group,the value of microvessel density (MVD) decreased and the expression of PCNA significantly decreased versus other groups. Conclusion Endostar may normalize the tumor vasculature.And the time window is found at Days 4 -6 post-treatment.During the time of vascular normalization,a combination therapy of endostar plus cisplatin has optimal efficacies.

Objective To construct dual fusion reporter gene expression vector containing enhanced green fluorescence protein (EGFP) and human transferrin receptor (TfR),and validate the reconstructed plasmid,which will provide experimental foundation for in vivo dual-modality optical/Magnetic Resonance (MR) imaging.Methods Clone TfR into the pEGFP-C1 vector to construct pEGFP-C1-TfR plasmid.pEGFP-C1-TfR plasmid was transfected into 293T cells for 48 h,then investigate EGFP expression under a fluorescence microscope; detect TfR expression through PT-PCR; inspect the subcellular location of EGFP-TfR fusion protein through Confocal Scanning Laser Microscopy; evaluate the function of EGFP-TfR fusion protein through Tf probe uptake and competition assays.Results DNA sequencing analysis confirmed that EGFP-TfR gene sequence was correct,and there was no mutation and deletion.After transfecting the reconstructed plasmid into 293T cells, fluorescence microscope observation and RT-PCR results demonstrated that EGFP and TfR were expressed efficiently.EGFP-TfR fusion protein was located predominantly in the cellular membrane,and could specifically mediate internalization of Tf.Conclusion EGFPTfR dual fusion reporter gene expression vector has been successfully constructed,and could be expressed efficiently with functional features.Thus,the expression vector could be applied for in vivo dual-modality optical/Magnetic Resonance (MR) imaging.

Objective To explore the effects of urantide,a urotensin Ⅱ receptor (UT) inhibitor,on lipopolysaccharide (LPS)/D-gaiactosamine (D-GalN)-induced acute hepatocyte apoptosis in mice.Methods Male BALB/c mice were randomly divided into 4 groups ( n =6 each):normal control,pre-treatment control,model and pre-treatment model.The pre-treatment control and pre-treatment model groups received urantide (0.6 mg/kg body weight) by a caudal vein injection.At 30 minutes post-injection,the model and pre-treatment model groups were treated with LPS/D-GalN to induce acute hepatocyte apoptosis via an intraperitoneal injection.Hepatocyte apoptosis was examined by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) and caspase-3 colorimetric assay.The expressions of proinflammatory cytokines,such as tumor necrosis factor alpha (TNF-t),interferon-γ ( 1FN-γ) and interleukin-1 beta (IL-1β),were detected by reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay.Results Massive hepatocyte apoptosis were detected in the model group.The apoptotic index was clearly reduced in the pre-treatment model group [ ( 26 ± 11 ) ％ vs (77 ±20) ％,P ＜0.01 ].And the activity of caspase-3 was also lower in the pre-treatment model group than that in the model group [ ( 2.50 ± 0.83 ) pmol · min- 1 · mg- 1 vs ( 3.76 ± 0.42 ) pmol · min -1 · mg- 1,P ＜0.01 ].In addition,the serum and liver tissue levels of TNF-α,IL-1β and IFN-γin the pre-treatment model group were significantly lower than those in the model group [ 1.69 ± 0.47 vs 3.57 ± 0.79,0.31 ±0.02 vs 0.46 ± 0.06,2.81 ± 0.72 vs 3.35 ± 0.84,(233 ± 36 ) pg/ml vs (441 ± 157 ) pg/ml,(228 ±21) pg/ml vs (364 ±20) pg/ml,(93.8 ±5.2) pg/ml vs (180.3 ±4.3) pg/ml,allP＜0.01].Conclusion LPS/D-GalN-induced acute hepatocyte apoptosis can be inhibited by a pretreatment of urantide through an inhibition of expression and secretion of proinflammatory cytokines.The U Ⅱ/UT receptor system plays a pivotal role in the liver immuno-inflammatory injury of acute liver failure (ALF).And it may become a new drug target of ALF therapy.

Objective To assess the methods of establishing an orthotopic nude murine model of human pancreatic cancer and explore the manifestations of MRI (magnetic resonance imaging) and its pathological fundamentals so as to provide research rationales for human pancreatic cancer.Methods The BXPC-3 cell of human pancreatic cancer was orthotopically planted in nude mice.And the animals were examined by a clinical 3.0 T magnetic resonance imager.The MRI examinations were analyzed along with their pathological findings.Results Among 18 mice,15 of them grew tumors with a success ratio of 83.3％.The pathological findings conformed to those of high differentiation pancreatic parenchyma cancer.Comparing with the neighbor muscles, the tumors were homogeneous (66.7％, 10/15 ) or heterogeneous (33.3％,5/15) of hypointense on Tl-weighted images while homogeneous (26.7％,4/15)or heterogeneous ( 73.3％, 11/15 ) hyperintense on T2-weighted images with heterogeneous enhancement.The border became obviously enhanced and there was mild central enhancement while the necrotic part showed no enhancement.Conclusion 3.0T MRI can detect pancreatic neoplasms ≥2 mm and visualize clearly their locations,shapes,dimensions and internal structures in an orthotopic nude murine model.Thus it provides a visible framework for further studies of human pancreatic cancer.

Dear editorial department of Chinese medical journal: The research results of Professor Wu Xueyan's research group [1] "The value of triptorelin stimulation test in evaluating the function of male hypothalamus-pituitary-gonad axis" were published in the 10th issue of your journal in 2011. Under the current situation that gonarelin is often out of stock or even facing discontinuation in China, this research result has high academic and application value for pediatric endocrinologists engaged in clinical work and research on childhood and adolescence development, and provides a new choice that can replace gonarelin stimulation test in diagnosing adolescent development abnormalities.

Clinically, GERD can BE divided into three types: non-erosive reflux disease (NERD), reflux esophagitis (RE) and Barrett's esophagus (BE). The prevalence rate is 20% ~40% in western countries and 5% ~17% in Asia. In recent years, the prevalence rate in Asia is increasing year by year [1]. Proton pump inhibitors (PPI) are considered to BE the most effective and commonly used drugs for the treatment of GERD [2]. Most previous studies have focused on RE, and in recent years, NERD has been paid attention to. It has been found that only a few GERD patients have esophagitis or BE manifestations under gastroscopy, and most GERD patients have NERD, that is, no esophagitis manifestations under endoscopy. In Asian countries, this situation may BE more prominent, and NERD can account for about 2/3 of the total number of GERD patients. However, the therapeutic effect of PPI on NERD is far less than that of RE [3]. Only 30% ~60% of NERD have symptoms relieved after taking PPI [3-5], and some research data are even lower, only 30% ~35% [6]. Therefore, more than half of NERD patients are not sensitive to PPI treatment. In clinical practice, some GERD patients

Genomics studies have shown that mammalian genomes are characterized by extensive and dense transcription. Less than 2% of them are transcribed into proteins, while more than 98% are transcribed into non-coding RNA (ncRNA) [1]. ncRNA can be divided into small non-coding RNA (small ncRNA) and long-chain non-coding RNA (LncRNA) according to their length. At present, the research hotspots are still focused on ncRNAs such as microRNAs and small interfering RNAs, while the research on lncRNAs is still in a relatively unfamiliar field. Although lncRNAs were once considered to be by-products of RNA polymerase II transcription, they are the "noise" of genome transcription and have no biological function [2], in recent years, more and more studies have shown that lncRNAs can widely participate in genome regulation, such as X chromosome inactivation, genome imprinting, chromatin modification, transcription, splicing, translation, degradation, transport, etc., thus widely participate in the regulation of individual growth and development, apoptosis, proliferation, differentiation and other life activities, and are inextricably related to many diseases including tumors [34]. Now, the mechanism and research progress of lncRNAs in the process of tumor development are reviewed.

Alzheimer's disease (AD) is the most common cause of senile dementia, and there is no effective prevention and treatment method for the etiology at present. amyloid-beta (A β) plays a key role in the occurrence of AD, and clearing A β in the brain has become one of the important strategies for the prevention and treatment of AD [1]. In recent years, important progress has been made in anti-A β immunotherapy, and the present situation, problems and future of anti-A β immunotherapy are reviewed and prospected in this paper.

Dear editorial teacher, hello! The article "The Value of Triptorelin Stimulation Test in Evaluating the Function of the Hypothalamus-Pituitary-Gonadal Axis in Men" published in the 10th issue of your journal in 2011 did not describe the drug route and drug dosage in the triptorelin stimulation test in detail because of layout limitations and other factors. Here, we add the following explanation.

A 45-year-old male was found to have a mass in his right thigh for 2 years. Two years ago, the patient inadvertently touched a mass about the size of an egg in his right thigh, without discomfort and no attention, but it is still progressively enlarged. X-ray and CT in the external hospital showed that the right thigh soft tissue tumor was accompanied by femoral bone destruction, and he came to the Second Affiliated Hospital of Wenzhou Medical College on September 15, 2004. Physical examination showed that the middle and anterior side of the right thigh was obviously swollen, hard when touched, and local skin showed surgical scar. Past history: 6 years ago, he underwent internal fixation for right femoral fracture in the external hospital, and the plate was removed 1 year after operation, and the healing was good.

Objective To prepare compound epirubicin hydrochloride-loaded polymeric nanoparticles of L-lactic-co-glycolic acid and establish their quality control.Methods The emulsion-solvent evaporation method was employed to prepare and freeze-dry the compound epirubicin hydrochloride-loaded polymeric nanoparticles of L-lactic-co-glycolic acid after the addition of lactose.The contents and cumulative release of epirubicin hydrochloride and dimeglumine gadopentetate were detected simultaneously by RPHPLC (reverse phase-high performance liquid chromatography).Results The above nanoparticles were prepared and the quality standards for simultaneously determining the contents of epirubicin hydrochloride and dimeglumine gadopentetate established primarily.The contents of epirubicin hydrochloride and dimeglumine gadopentetate in compound preparation were 100.6％ ± 1.6％ and 99.1％ ± 1.9％respectively.And two compositions could be completely released within 9 days.Conclusion The preparation method of nanoparticles is simple and their quality control feasible.