Volume 93  ·   Issue 38  ·  2013

To explore the protein-tyrosine-phosphatase 1B (PTP1B) expression and its prognostic significance in non-small-cell lung cancer (NSCLC).

Immunohistochemical method of En Vision was applied to investigate the expression of PTP1B in lung specimens from 63 cases with NSCLC and 9 cases with pneumonia confirmed by pathological examination after surgical removals at Jinling Hospital from June 2000 to October 2010.The data of PTP1B expression in different lung tissues and its relationship with clinicopathological features and prognostic value for survival were analyzed by chi-square test, Kaplan-Meire survival analysis, Log rank test and multivariable Cox regression analysis.

The expression of PTP1B was negative in pneumonia group while positive in NSCLC tissues (32/63, 50.8%)(χ²=8.229, P=0.004).The expression of PTP1B in NSCLC tissue was not correlated with gender, age, smoking history, tumor size, pathological type, lymph node metastasis and chemotherapy, but significantly associated with p TNM staging (χ²=6.426, P=0.040).Kaplan-Meire survival analysis showed that the expression of PTP1B was significantly correlated with overall survival (Log-rank, P=0.047).Multivariable Cox regression analysis indicated that both PTP1B (HR=2.050, P=0.044) and p TNM staging (HR=3.631, P=0.000) were independent prognostic factors for NSCLC.

PTP1B may be a biomarker for differential diagnosis of malignant lung disease.And PTP1B and p TNM were independent prognostic factors for NSCLC.

To explore the diagnostic efficiency of percutaneous lung biopsy for malignant lung tumors under the guidance of computed tomography (CT).

The clinical data of 623 patients undergoing CT-guided percutaneous lung biopsy at Department of Respiratory Medicine, Xinqiao Hospital, Third Military Medical University from January 2010 to November 2012 were analyzed to examine the influencing factors for the occurrences of pneumothorax and hemoptysis, including patient age, gender, site of puncture, tumor location and size, depth and number of puncture, chronic obstructive pulmonary disease (COPD) and doctor′s clinical experience.

A total of 623 cases were recruited.There were 410 male and 213 female with an average age of 60±11 (15-87) years.Among them, 618 were diagnosed as malignant lung tumors with a positive rate of 99.2%.Pathological classifications were adenocarcinoma (n=387, 62.1%), squamous carcinoma (n=166, 26.6%), small cell lung cancer (n=30, 4.8%), undifferentiated cancer (n=18, 2.9%) adenosquamous carcinoma (n=2, 0.3%), sarcomatoid carcinoma (n=6, 0.9%), leiomyosarcoma (n=3, 0.5%), neuroendocrine carcinoma (n=2, 0.3%), low differentiated carainoid (n=1, 0.2%),cribriform carcinoma (n=1, 0.2%), malignant lymphoma (n=1, 0.2%) and metastatic renal cell carcinoma (n=1, 0.2%) respectively.Another 5 patients (0.8%) had an initial diagnosis of chronic inflammation according to their pathological features of lung punctures.Their definite diagnoses were lung squamous cell carcinoma (n=3), adenocarcinoma (n=1) and small cell carcinoma (n=1) respectively.The major complications were pneumothorax and hemoptysis.Among 73 cases of pneumothorax (n=73, 11.7%), 65 cases (n=65, 10.4%) of mild pneumothorax (lung compression<20%) were cured by sufficient oxygen inhalation and repose while 8 cases (1.3%) suffered lung compression≥75% recovered via closed thoracic drainage.Eighty-four patients (13.5%) suffered from the complications of slight or self-limited hemoptysis with blood-tinged sputum.The symptoms of hemoptysis disappeared after oral hemostatic or other treatments.Another 5 patients (0.8%) suffered from moderate hemoptysis (100- 500 ml) and their symptoms disappeared after rest or an intravenous infusion of haemostatic.None of them died of massive hemoptysis or biopsy.The occurrence of pneumothorax was significantly correlated with the diameter of lesions (≤2 cm)(P=0.006), depth of needle (>3 cm)(P=0.044), number of puncture needles (≥3 needles)(P=0.025), inadequate clinical experience of puncturing doctors (P=0.003) and COPD (P=0.036); the occurrence of hemoptysis was obviously correlated with the diameter of lesions (≤2 cm)(P=0.031), depth of needle (>3 cm)(P=0.018), number of puncture needles (≥3 needles)(P=0.015) and inadequate clinical experience of puncturing doctors (P=0.014).However, the relationship was unremarkable between the occurrences of pneumothorax and hemoptysis and patient gender, age, sites of puncture and tumor location.

With a high diagnostic value, CT-guided percutaneous lung biopsy is both safe and effective for malignant lung tumors.

To evaluate the application value of ¹⁸F-fluorodeoxyglucose (¹⁸F-FDG) positron emission tomography/computed tomography (PET/CT) in early diagnosis of lung cancer.

Retrospective analyses were conducted for 347 cases of pulmonary space-occupying lesions at Zhongshan Hospital from June 2010 to October 2012.The diagnostic validity of PET/CT and fluorodeoxyglucose maximum standardized uptake value (SUV_max) of lesions were compared respectively.Among different morphological characteristics, pathologic types and levels of tumor markers.The diagnostic value of PET/CT was also evaluated along with serum tumor markers for lung cancer.

SUV_max was positively correlated with lesion size (r=0.484, P<0.05) and negatively with tumor differentiation degree (r=-0.232, P<0.01).It was significantly higher in tumor marker positive group than the negative group (10.6±5.5 vs 7.6±5.4, P<0.05).The diagnostic specificity, sensitivity and accuracy of PET/CT were 50.0%, 96.6% and 89.3% in lung cancer.And the greater the lesion, the higher the diagnostic accuracy (P<0.05).PET/CT plus serum tumor markers could boost the diagnostic specificity of lung cancer by 30% (P<0.01).

¹⁸F-FDG PET/CT has high diagnostic values for early stage pulmonary nodules.It can also suggest the differentiation degree of lung cancer.Combined use of serum tumor markers and ¹⁸F-FDG PET/CT increases the early diagnostic specificity of lung cancer.

To explore the correlation between computed tomographic (CT) vascular convergence sign and enhancement value in patients with pulmonary nodules.

A total of 708 consecutive patients with pulmonary nodule received dual-source CT scan from January 2010 to January 2012.They were divided into vascular convergence sign group (including 4 subgroups) and non-vascular convergence sign group.Then the correlation between CT vascular convergence sign and enhancement values was analyzed.

The enhancement values in vascular convergence sign group were significantly higher than those in non-vascular convergence sign group ((27.6±10.5) vs (3.2±2.8) HU, P=0.000).The CT enhancement values in lesions tended to increase with the number of connecting blood vessels.However, no significant differences existed among the subgroups (P>0.05).The accuracy of vascular convergence sign for detection of pulmonary malignant nodules was 84.9%,70.6% and 60.3% according to the standards of CT enhancement values≥15, 20, 25 HU respectively.The sensibility, specificity and accuracy of determining pulmonary malignant nodules were 97.2%, 68.8% and 93.7% according to the standard of vascular convergence sign.The accuracy of determining pulmonary nodules′CT enhancement values≥15 HU was 88.1% according to the standard of vascular convergence sign.

Vascular convergence sign may be used to indicate the enhancement of pulmonary nodules when CT enhancement images are not available.

To explore the application value of low-dose spiral computed tomography (LDCT) in lung cancer screening.

A total of 2251 asymptomatic subjects undergoing chest LDCT scan at Center of Physical Examination, Affiliated Zhongshan Hospital, Fudan University between June 2011 and December 2012 were prospectively enrolled.The incidence rates of lung nodule and lung cancer were analyzed to compare the value of LDCT screening in subjects with smokingrelated high, medium and low risks of lung cancer.The value of serum tumor biomarker in the reduction of false positive of LDCT was also discussed.

Among all subjects, 9.9% (222/ 2251) displayed at least 1 non-calcified nodule with a diameter≥4 mm.Two subjects were diagnosed with lung cancer and 1 of them received surgical resection.Other subjects with lung nodules were followed.There was no statistical difference in the incidence rates of lung nodule between the high, medium and low-risk groups of lung cancer associated with smoking (8.8%, 9.5% and 10.1%, P=0.864).The incidence rates of lung nodule in subjects≥55 years old were higher than that of those<55 years old (12.7% vs 9.1%, P=0.034).Female gender had a high risk of ground glass opacity (GGO) or ground glass nodule (GGN)(P=0.015).The independent or combined increase of serum tumor biomarkers of carcinoembryonic antigen (CEA), neuron specific enolase (NSE), cytokeratin fragment 21-1 (Cyfra211) and squamous cell carcinoma antigen (SCC) might not predicate the incidence of lung nodule.

LDCT screening is highly valuable in lung cancer screening.

To analyze the effects of preoperative uses of aspirin and clopidogrel on perioperative blood loss and blood transfusion requirements and complication in patients undergoing off-pump coronary artery bypass graft (CABG).

At our hospital from October 2011 to October 2012, a total of 480 patients underwent off-pump CABG performed by the same surgical team.Among them, 198 patients continued aspirin (discontinued clopodgrel at least 5 days) through operation (aspirin group,1), 53 had aspirin and clopidogrel until 3 days before surgery (aspirin and clopidogrel group, 2) and 229 discontinued antiplatelet therapy 5 more days before surgery (control group, 3).

No significant difference of basic clinical characteristics existed among three groups (P>0.05).Group 2 was associated with a greater volume of chest tube drainage than other groups (827±216 vs 416±135 vs 265±85 ml, P<0.05).There was no significant difference between groups 1 and 3, even though chest tube drainage volume of group 1 was greater (827±216vs 265±85 ml, P>0.05).There was no instance of stroke, myocardial infarction or post-operative mortality.

Preoperative clopidogrel exposure within 5 days of surgery increases perioperative blood loss.And the pre-operative use of aspirin results in no difference in postoperative perioperative blood loss and cardiovascular outcomes.

To explore the serum levels and significance of hypoxic inducible factor (HIF)-1α and HIF-2α in Tibetan and Han residents living at different altitudes.

A total of 100 healthy adults of Han in plain (altitude 5 m, group P) and Tibetan and Han at moderate altitude (2260 m, groups M1& M2) and high altitude (4380 m, groups H1& H2)(n=20 each) were selected from June 2010 to August 2010.Venous blood samples were collected in the morning.The serum concentrations of HIF-1α and HIF-2α were measured with enzyme-linked immunosorbent assay (ELISA) to analyze the correlations of HIF-1αand HIF-2α with arterial partial pressure of oxygen (PaO₂) and hemoglobin (Hb).

The level of HIF-1α in group H2 ((6.06±1.85) μg/L) was higher than that in groups P ((4.56±0.85) μg/L, P<0.05), M1 ((4.41±1.05) μg/L, P<0.01) and M2 ((4.59±1.03) μg/ L, P<0.05).The level of HIF-1αin group H1 ((5.27±0.98) μg/L) was higher than that in group M1 (P<0.05).The level of HIF-2α in group H2 ((0.83±0.48) μg/ L) was higher than that in groups P ((0.33±0.11) μg/L, P<0.01), M1 ((0.14±0.06) μg/L, P<0.01) and M2 ((0.24±0.11) μg/L, P<0.01).The level of HIF-2αin group M1 was lower than that in groups P (P<0.01) and M2 (P<0.01).The level of HIF-2αin group H1 ((0.18±0.16) μg/L) was lower than that in groups P (P<0.05) and H2 (P<0.01).The levels of HIF-1α and HIF-2α were negatively correlated with PaO₂ (r=-0.475,-0.551, both P<0.01) and positively with Hb in Han groups (r=0.433, 0.463, all P<0.01).The levels of HIF-1α and HIF-2αhad no correlation with PaO₂ in Tibetan groups (r=-0.270,-0.198,both P>0.05).No correlation existed between HIF-1α and Hb in Tibetan.A positive correlation existed between HIF-2αand Hb in Tibetan (r=0.325, P<0.01).

The changes of HIF-1αand HIF-2αvary for Tibetan and Han residents at different altitudes.And the level of HIF-2αis consistent with the change of Hb.As compared with HIF-1α, HIF-2α may play a more important role in regulating Hb.

Analysis the result of human mandible and temporomandibular joint using two different three-dimensional finite element method under different mechanical models.

The 3-dimensional model including cortical and cancellous bone for human mandible was obtained through computed tomography (CT) scan.Then the model was meshed in the software ICEM CFD.The passive and active muscle-force loadings were separately applied on the FE model to simulate the anterior clenching.Stress distributions in two models were compared.

The stress distributions of two models were apparently different.In the passive muscle-force model, high stress was mainly distributed in mandibular angle, retromolar trigone, notch and bite point on crown.In the active muscle-force model, high stress was mainly distributed in condylar vertex and neck, mandibular angle, retromolar trigone and bite point on crown.There were some similarities between passive and active muscle loadings.However, large difference existed in condylar region due to the vertices reaction force disparity.

Closer to actual stressing state of human mandible and temporomandibular joint, the active muscle-force model is a proper biomechanical model for human mandible under anterior clenching.

To explore the prognostic significance of early monitoring of BCR-ABL transcript levels and cytogenetic evaluations for chronic myeloid leukemia in chronic phase (CML-CP).

From July 2007 to May 2012,56 CML-CP patients received oral imatinib 400 mg/d.The BCR-ABL transcript levels were monitored and cytogenetic examinations performed after 3 and 6 months respectively.The median follow-up time was 48 months.

The 3-month BCR-ABL transcript levels≤10% of patients 5-year overall survival (OS) and progression-free survival (PFS) were better than BCR-ABL transcript levels>10% of patients (OS: 100% vs 84.6%, P=0.011; PFS:94.6% vs 67.7%, P=0.045); cytogenetics:Ph⁺≤35% of patients 5-year OSand PFSbetter than Ph⁺>35% of patients (OS: 100% vs 76.2%, P=0.001; PFS: 95.2% vs 38.1%, P=0.001); the 6-month BCR-ABL transcripts level≤1% of patients 5-year OS and PFS also better than BCR-ABL transcript levels>1% of patients (OS: 100% vs 71.4%, P=0.000; PFS: 95.2% vs 47.6%, P=0.001); Ph⁺=0% and Ph⁺>0% patients, 5-year OS and PFS were significantly different (OS: 100% vs 68.6%, P=0.000; PFS: 95.3% vs 45.7%, P=0.000).Conclusions Early molecular biology and cytogenetics monitoring have some significance in the prognostic assessment of CML-CP.And individualized treatment strategies should be based upon the monitoring results in conjunctions with comprehensive judgments.

To explore the clinical predictive factors of coronary artery stenosis located distally to myocardial bridging (MB).

A total of 603 patients with MB-mural coronary artery (MB-MCA) diagnosed by angiography initially were enrolled during May 2004 to May 2009.Their angiographic and clinical data were collected according to an uniform protocol.And standard questionnaires were used to acquire demographic information and clinical examinations.Univariate and multivariate analysis were performed to explore the related clinical predictive factors.

A total of 644 MB-MCAs were detected.Diastolic vessel diameters in MCAs were significantly smaller than those in reference segments ((2.29±0.39) vs (2.48±0.40) mm,P<0.001).Lesions located distally to MB detected in 36 patients were significantly fewer than those proximally to MBin 382 patients (5.9% vs 62.4%,P<0.001).Univariate analysis suggested that the narrowing degree of vessel located proximally to MB, the narrowing degree and the number of diseased coronary vessels of non-MB-MCAs and course of hypertension were positively correlated with the narrowing degree of vessel located distally to MB (all P<0.05).Multivariate Logistic regression analysis suggested that the number of cigarettes per day, the narrowing degree of diseased coronary vessels of non-MB-MCAs, the narrowing degree of vessel located proximally to MB and diastolic narrow rate of MCA were positively correlated with the occurrence of coronary artery stenosis located distally to MB (all P<0.05).Their standardized coefficients (β) were 0.763,0.727,0.420 and 0.403 respectively.And the corresponding Exp (β) were 2.146 (1.089-4.229), 2.070 (1.371-3.125), 1.521 (1.050-2.204) and 1.496 (1.094-2.045).

The number of cigarettes per day, the narrowing degree of diseased coronary vessels of non-MB-MCAs, the narrowing degree of vessel located proximally to MB and diastolic narrowing rate of MCA are likely to be important clinical predictive factors of coronary artery stenosis located distally to MB.

To explore the efficacies of nasal cavity enlarging surgery in obstructive sleep apnea hypopnea syndrome (OSAHS) patients with nasal obstruction.

From April 2011 to October 2012, a total of 22 OSAHS patient with nasal obstruction underwent nasal cavity enlargement.Natural overnight snoring was digitally recorded, polysomnography (PSG) performed simultaneously and nasal resistance measured at pre- and post-operation.Body mass index (BMI), Epworth sleepiness scale (ESS), apnea-hypopnea index (AHI), minimum oxygen saturation SaO₂, total nasal resistance, peak frequency, maximal frequency, mean frequency, central frequency and power ratio 800 Hz of snores were recorded and compared.

BMI and SaO₂ rose while AHI and ESS declined post-operatively.No statistical difference existed between pre-operation and postoperation.The total nasal resistance was (0.39±0.15) and (0.29±0.11) Pa·cm^-3·s^-1 at pre- and post-operation.And there was significant statistical difference (P<0.01).The snores at pre- and post-operation exhibited different patterns in time and power spectrum distribution domains.And fmax, fpeak and fmean declined while fc and PR800 rose at post-operation.Significant statistical differences existed in the change of fpeak and PR800 at pre- and post-operation (P<0.01).

Nasal cavity enlargement can lessen nasal resistance and change the acoustic characteristics of snoring in OSAHS patients.The postoperative snoring focus of lower frequency suggests that acoustic parameters of snores may be used to evaluate the efficacy of OSAHS surgery.

To compare the treatment efficacy of imatinib mesylate versus allogeneic hematopoietic stem cell transplantation (allo-HSCT) for patients with chronic myeloid leukemia in chronic phase.

The efficacy, overall survival, progression-free survival and adverse events were evaluated in 198 patients on these two therapies from February 2002 to December 2012 at our hospital.One hundred and fifteen cases in imatinib group (n=115) received imatinib at an initial daily dose of 400 mg and then dose was adjusted according to blood routine test and therapy response.All patients were evaluated for hematologic, cytogenetic and molecular responses every 1-3 months.The allo-HSCT group (n=83) received myeloablative preconditioning regimen and methotrexate (MTX) and cyclosporine A (CsA) were used for graft-versus-host disease (GVHD) partially plus mycophenolate mofetil (MMF) and antihuman thymocyte globulin (ATG).The engraftment evidence and evolution of cytogenetic and molecular response was conventionally detected after allo-HSCT.

In imatinib group, 59 of 86 (68.6%) cases achieved complete cytogenetic response (CCyR) in the 12 months after therapy, while 67 of 70 (95.7%) cases achieved CCyR in allo-HSCT group.The relapse rates of two groups were 14.8% (17/115),12.3% (10/81) respectively.The adverse reaction of imatinib in imatinib group was obviously much more tolerable for patients compared with frequently occurred GVHD and infection in allo-HSCT group.The 10-year cumulative overall survival (OS) rate was 93.9% in imatinib group and 77.1% in allo-HSCT group (P=0.015).And the 10-year cumulative progression-free survival (PFS) rate was 86.1% in imatinib group versus 88.0% in allo-HSCT group (P=0.508).For Sokal rating stratified analysis, the cumulative OS rates of two groups were 96.4% and 68.0% (P=0.049) for intermediate-risk patients, 92.6% and 57.1% (P=0.017) for high-risk patients while the cumulative PFS rates of two groups were 89.3% and 88.0% for intermediate-risk patients (P=0.942), 70.4% and 85.7% for high-risk patients (P=0.405).The rates of OS and PFS were not significantly different for low-risk patients.The cumulative OS rates of two groups were 94.7% and 73.5% (P=0.009) for those≥30 years old and the cumulative PFS rates of two groups 84.2% and 94.1% respectively (P=0.147).

Imatinib mesylate is superior to allo-HSCT for patients with chronic myeloid leukemia in chronic phase.

To prospectively evaluate the health status of infants with exposure to melamine-contaminated milk formula prior to September 2008.

The cohort study was conducted in an area close to the manufacturer of Sanlu dairy products.There were three groups (n=47 each).In September 2008, the exposure groupⅠ included infants with exposure to melamine and a diagnosis of renal abnormalities, the exposure groupⅡ had exposure to melamine but there was no diagnosis of renal abnormalities and the non-exposure group had no exposure to melamine.The exposureⅡ and non-exposure groups were matched with those of exposure groupⅠby birthplaces, gender and date of birth (±3 months).Kidney function tests (urea nitrogen, serum creatinine, uric acid, serum albumin,β2-microglobulin and cystatin C), liver function tests (alanine aminotransferase and aspartate aminotransferase), growth and development assessment and urinary system ultrasonography were implemented between November 2011 and June 2012.The analysis of covariance (least significant difference method) was performed to compare the differences of relevant variables among three groups.

The urinary system ultrasonography showed that all abnormalities disappeared in exposure groupⅠ and all infants of another two groups had normal ultrasonography.There were statistically significant differences in serum uric acid and albumin of kidney function in exposure groupⅠ, exposure groupⅡand non-exposure group ((344±75) and (338±98) and (282±69) μmol/L, (47±5) and (47±6) and (43±5) g/L, all P<0.05) among three groups.However the differences in the remaining markers of kidney function, markers of liver function and Z scores of weight-for-age and height-for age were all statistically insignificant (all P>0.05).Further pair-wise comparisons showed that the levels of serum uric acid and albumin in exposure groupⅠ were higher than those in non-exposure group (P=0.001 and 0.010).And the levels of serum uric acid and albumin in exposure groupⅡ were also higher than those in non-exposure group (P=0.003 and 0.005).

All affected infants have recovered from kidney abnormalities.Early infant exposure to melamine-contaminated milk formula appears to have no obvious impact on liver function and growth.But its potential impact on kidney function should be further assessed.

To explore whether bone marrow transplantation (BMT) can restore gastrointestinal mast cells in mast cell deficient (Ws/Ws) rats and understand the features of these reconstituted mast cells.

Thirty-six Ws/Ws rats were subjected to Co⁶⁰ radiation at 6 gradient doses (6.0, 7.0, 8.0, 9.0, 10.0 and 11.0 Gy, n=6) to confirm the appropriate dosage. And another 6 rats served as non-radiated controls.Sixteen Ws/Ws rats were exposed to a 7.5 Gy radiation and sacrificed at Day 1,5,8 and 12 (n=4) to obtain hemogram and myelogram. And 4 nonradiated Ws/Ws rats served as control.Ws/Ws rats received an intravenous injection of bone marrow cells harvested from healthy cogenic Brown Norway (BN) rats after a dosage of 7.5 Gy radiation.Ychromosome fluorescence in situ hybridization (Y-FISH) was used to identify the survival and differentiation of bone marrow cells of male BN rats in female BMT-Ws/Ws rats (n=4).Another set of 24 male BMT-Ws/Ws rats were sacrificed Weeks 5, 8,13 and 23 post-BMT (n=6).Tissues from esophagus, stomach, ileum and colon were harvested to perform mast cell quantification by Alcian blue staining.And mast cell derived proteinases (tryptase and chymase) were quantified by Western blotting.

Y-FISH showed bone marrow cells from male BN rats survived in female BMTWs/Ws rats and mast cells were restored in gastrointestinal tract.Compared with control, the highest level of mast cell number (103±6) vs (35±4)/mm² for esophagus, (271±23) vs (124±13)/mm² for stomach, (200.1±13.3) vs (103.2±6.6)/mm², all P<0.05) and mast cell proteinases (1.3±0.3 vs 0.6±0.2 for esophagus, 3.6±0.8 vs 1.9±0.4 for ileum, both P<0.05) in BMT-Ws/Ws rats were observed at Week 8 post-BMT.The number of mast cell and proteinases decreased at Week 13 post-BMT.

BMT may restore mast cells in Ws/Ws rats.The best time period for using BMT-Ws/Ws rats in the study of mast cell function is between Weeks 8-13 post-BMT.

To explore the expression of poly (ADP-ribose) polymerase/nuclear factor-κB ( PARP/NF-κB) and intervention effect of 5-aminoisoquinolinone/pyrrolidine dithiocarbamate (5-AIQ/PDTC) in severe acute pancreatitis (SAP) rats with adrenal damage.

The primarily cultured adrenocortical cells were quantitatively divided into control group (SO), pancreatitis group (SAP), PDTC drug control group (SO+PDTC), PDTC intervention group (SAP+PDTC), 5-AIQ drug control group (SO+5-AIQ) and 5-AIQ intervention group (SAP+ 5-AIQ).The SAP and 2 intervention groups were stimulated with the sera of SAP rats.Then corresponding drugs were added and culture continued for 12 hours.The corticosterone levels and PARP/NF-κB expression were observed for each group.

Adrenal cells in vitro cultured were round or oval, had secretory granules and could be stained by 3β-hydroxysteroid dehydrogenase antibody.The adherence rate was 60% after 48-hour culturing.The corticosterone level of SAP group was significantly lower than that of SO group[ (216.4±15.7) vs (294.8±16.3) μg/L, P<0.05].The 2 intervention groups were (258.6±19.0) and (264.3±18.2) μg/L respectively.These two values were higher than those of SAP group (P<0.05), but lower than those of SO group (P<0.05).With regards to the expression of PARP-1, the SAP and PDTC intervention groups were higher than SO group while 5-AIQ intervention group was significantly lower than SAP and PDTC intervention groups, but higher than SO and drug control groups.The expression of NF-κB in SAP group was higher than that in SO group.Two intervention groups were lower than SAP group, but higher than SO and drug control groups.

The pathway of PARP/NF-κB participates in adrenal damage of SAP rats.To a certain extent, the uses of 5-AIQand PDTC may alleviate adrenal damage.