Volume 89  ·   Issue 15  ·  2009

In the 1940s, professors Xie Rong, Wu Jue and Shang Deyan, who studied abroad in the United States, really introduced modern anesthesiology theory and technology into China [1]. In the 1950s, they established professional anesthesiology departments and anesthesia teaching and research offices in Shanghai and Beijing. With the progress of science, the intravenous anesthetic thiopental was introduced in 1932. On the basis of a lot of experimental research and clinical practice, Professor Xie Rong first applied pediatric intramuscular thiopental basic anesthesia to pediatric surgery in China [2].

Objective To investigate the effects of cantiopulmonary bypass (CPB) on plasma pentraxin-3 (PTX3) level and balance of Th1/Th2 function in children with congenital heart malformations. Methods 16 children with congenital heart malformations, 9 male and 7 female, aged 3-52 months, weighing 3.4-14. 5 kilograms, classified as Ⅱ or Ⅲ ASA grades, underwent operation under CPB. Blood samples were collected after anesthesia induction, 5 min and 30 min since beginning of CPB, and 5 min, 2 h, 4 h, 8 h, and 16 h after weaning from CPB. ELISA was used to examine the plasma concentrations of interferon (INF)-γ, interleukin (IL)-4, and PTX3. The ratio of INF-γ/IL-4 was calculated. Results The INF-γ level began to increase significantly 5 rain since CPB (P <0.05), peaked 5 min after weaning from CPB (P<0.01), then gradually decreased, and returned to the level before CPB 16 h after the weaning from CPB (P>0.05). The IL-4 level began to increase significantly 30 min since CPB (P<0.01), peaked 2 h since CPB (P<0.01), however, still not returned to the level before CPB 16 h after the weaning from CPB (P < 0.05). The PTX-3 level was significantly higher 30 min since CPB and 5 min and 2 h after the weaning from CPB (all P < 0.01). The INF-γ/IL-4 ratio was significantly higher 5 min and 30 min since CPB and 5 min after weaning from CPB (all P>0.05). Conclusion CPB can leads to elevation of plasma concentration of PTX3 and drift of Th1/Th2 function equilibrium, thus resulting in immunity disequilibrium.

Objective To investigate the minimum alveolar concentration (MAC) of sevoflurane required for insertion of laryngeal mask airway (LMA) in anesthetized children. Methods 27 children, aged 3-8, with an ASA physical status Ⅰor Ⅱ, underwent elective surgery under general anesthesia induced by inhalation of 8% sevoflurane in oxygen. After loss of eyelash reflex, the designated end-tidal concentration of sevoflurane remained stable for at least 10 min, and then the LMA was inserted. Each target concentration at the time of insertion was predetermined by the Dixon up-and-down method (with 0.2% as a step size) starting at 2.0% end-tidal concentration of sevoflurane. Successful insertion was defined as excellent inserting condition (fully relaxed jaw, no coughing, and no movement of the limbs, no breath holding, and no laryngospasm). Results The mean MAC of sevoflurane to achieve successful LMA insertion in 50% of children was (2.01±0.19)% ,and the ED95 was 2.36% (95% CI 2.15%-4.15%).Conclusion LMA insertion can be safely accomplished without coughing, moving, or any other airway complications in 50% and 95% of children at 2.01% and 2.36% end-tidal concentrations of sevoflurane.

Objective To investigate the protective effects of postconditioning with different concentrations of sevoflurane on renal ischemia-reperfnsion (I/R) injury. Methods The following procedures were performed on 48 male SD rats to establish I/R models: the fight kidney and ureter were resected, and the left renal arteries and veins were occluded by atranmatie clamps for 45 rain and then the clamps were removed to provide reperfusion for 24 h. And the rots were randomly divided into 4 equal groups; I/R Group, S1.2 Group inbreathing 1.2% sevoflurane simultaneously since the beginning of reperfusion for 2 h, S1.8 Group inbreathing 1.8% sevoflurane simultaneously since the beginning of reperfusion for 2 h, and S2.2 Group inbreathing 2.2% sevoflurane simultaneously since the beginning of reperfusion for 2 h. Another 12 rots underwent resection of the right kidney and ureter and exposure of the left renal arteries and veins only for 45 min. The rates were killed at the end of 24h reperfusion. Blood samples were taken for determination of serum creatinine (Cr) and blood urea nitrogen (BUN). The left kidneys were harvested to undergo microscopy to observe the psrcentnge of pyknotic nuclei in cortical tubular cells. Immunohistochemistry was used to detect the expression of endothelial nitric oxide synthase (eNOS)and inducible nitric oxide synthase (iNOS) in the kidney. Results The necrotic rate of renal tubule of the Sham Group was significantly lower than those of the other 4 groups (all P <0.01), and the necrotic rates of renal tubule of the S1.2, S1.8, and S2.2 Groups were all significantly lower than that of I/R Group (all P <0.05). The levels of blood Cr and BUN, and the percentage of pyknotic nuclei in cortical tuber cells of I/R, S1.2, S1.8,and S2.2 Groups were all significandy higher than those of Sham Group(all P<0.05)and these indexes listed above of S1.2, S1.8, and S2.2 Groups were all significandy lower than those of I/R Group (all P <0.05). However, there were not significant differences in these indexes among S1.2, S1.8, and S2.2Groups (all P >0.05). The kidney eNOS and iNOS expression levels of I/B Group were both significantly higher than those of Sham Group (both P <0.05), and the kidney eNOS and iNOS expression levels of S1.8Group were both significsntly lower than those of I/B Group (both P < 0.05). Conclusion Sevoflurane postconditioning attenuates the renal damage induced by I/B and down-regulates the eNOS and iNOS expression in kidney. Different concentrations do not show different effects.

Objective To investigate the optimal concentrations of sevoflurane and propefol for laryngeal mask airway (LMA) insertion in Chinese boys of different ages. Methods 200 boys with ASA status Ⅰ weighting 7-53 kg undergoing inguinal operation were divided into 4 groups (n=50) according to age: Group A (aged 7m-1 yr), Group B (1-3 yrs), Group C (4-6 yrs), and Group D (7-11 yrs).No premedication was given. General anesthesia was induced with propefol intravenous infusion at target effect-site concentration by target controlled infusion (TCI) using children Marsh model (Subgroups As, Bs,Cs, and Ds, 25 cases for each subgroup)or sevoflurane inhalation combined with 50% N2O(Subgroups Ap, Bp, Cp, and Dp, 25 cases for each subgroup)the optimal concentration was predetermined according to the modification of Dixon's up-and-down method. The size of LMA was determined according to the boy's weight and reverse technique was used to place LMA. The insertion conditions were noted as satisfactory when the jaw was relaxed, lash reflex disappeared, no coughing, gagging, swallowing, and laryngespasm was seen, and limb movement was minimal. Results The levels of ED50 of PET-sevoflurane for LMA insertion in Subgroups As, Bs, Cs, and Ds were 2.96% (95% CI 2.58%-3.44%), 2.62% (95% Ci 2.31%-2.90%), 2.16% (95% CI 1.97%-2.33%), and 1.89% (95% CI 1.66%-2.24%) respectively. The levels of ED<,50 of target effect site concentration with propofol for LMA insertion in Subgroups Ap, Bp, Cp, and Dp were4.74 μg/ml (95% CI 4.49-5.13 μg/ml), 4.33 μg/ml (95% CI4.07-4.62 μg/ml),4.10 μg/ml (95% CI 3.90-4.31 μg/ml), and 3.65 μg/ml (95% CI 3.24-3.98 μg/ml)respectively. Conclusion The ED50 level of PET-sevoflurane or target effect site concentration with propofol for LMA insertion in Chinese boys decreases gradually along with the increase of age.

Objectives To summarize the clinical and pathological features of glycogen storage disease (GSD) type Ⅲ. Methods The clinical data of 12 GSD type Ⅲ, 8 males and 4 females, aged 2-27, were collected. The biopsy specimens of quadriceps muscle of thigh underwent HE and histochemical staining and light and electron microscopy. Results The main clinical feature were hepatomegaly and hypoglycemic symptoms, slow growth, and microsoma since childhood, while myopatby was mild. Laboratory findings included low plasma glucose (n = 12), high liver transamiuses (n = 12), increased CK (n = 11),mild metabolic acidosis (n = 11), hyperlipemia (n = 9), elevation of blood lactate (n = 5), high uric acid (n = 1), and decrease of serum camitine level (n = 1). One patient had echographic evidence of cardiomyopathy, 11 patients were postprandial adreanalin stimulation test positive. Raw corn starch therapy was used on all patients and showed effective on liver manifestations. Muscle biopsy showed vacuolar myopathy, PAS positive glycogen granules in muscle fibers, small loci of intense ACP reactivity, and deposit of lipid droplets. Conclusion GSD type Ⅲ exhibits a clinical heterogeneity. Besides hepatic symptoms, myopathy and cardiomyopathy should be addressed adequately. The degree of pathological change of muscles is not significantly related to the degree of functional impairment, duration of disease, and level of CK.

Objective To study the CT manifestations of the solid-pseudopapillary tumor of the pancreas (SPTP) and tu explore the value of post-processing in the surgery. Methods Clinical and CT features of 14 patients with SPTP proved pathologically were retrospectively analyzed. Results (1) 13tumors were composed of different proportions of solid and cystic parts and 1 tumor was composed of solid part only. The solid portions demonstrated slight enhancement in the arterial phase and marked enhancement in the portal venous phase on post-contrast CT. The cystic portions appeared hypo-attenuation on both pre-and post-contrast CT images. Tumors were encapsulated and the capsule enhanced markedly with well-defined margins on post-contrast images. (2) Dilatation of the common bile duct or pancreatic duct wasn't seen in all of the cases and 1 case with the right adrenal ganglioneuroma. (3) 5 tumors located in the head of the pancreas, 3 of which with the superior mesenteric vein compression and displacement, in 2 cases the vessels were partially encapsulated by tumor. 6 tumors located in the tail of the pancreas, 3 of them with splenic hilum compression and multiple splenic vein tortuesity and dilatation, the veins drained into superior mesenteric vein anteriorly and posteriorly of the tumor. Splenic vein compression and posterior displacement occurred in 2 cases. In one case, there was no clear margin between the tumor and the splenic hilum. Conclusion The SPTP has characteristic CT findings and correct diagnosis could be made by combining clinical features. The relationship of the lesions and surrounding structures could be revealed by the post-processing and was helpful for the operation.

Objective To evaluate the efficacy of EndostarTM (rh-endostatin,YH-16) combined with docetaxel and carboplatin (TP)regimen for the adjuvant treatment of non-small lung cancer (NSCLC) and its impact on circulating blood markers. Methods 36 patients with stage Ⅰ b-Ⅲ a postoperative NSCLC, were randomly divided into the treatment group, EndostarTM plus TP regimen, and the control group, TP regimen only, respectively. DFS and toxicities of patients were observed. The numbers of CEC and the levels of tumor marker CEA, NSE and CYFR21-1 were measured. Results The numbers of CEC and the levels of CEA, NSE and CYFR21-1 decreased after treatment. There were significant differences in GEC and NSE between treatment group and control group after four cycles of treatment, respectively (P = 0.016 and 0.013). Disease-free survival time (DFS) was longer in treatment group than control group but without significant difference. CEC was significantly increased in recurrent and metastasis cases and decreased after effective treatment Conclusion EndostarTM combined with TP regimen seem to be superior to TP alone in some short term index for the treatment of postoperative NSCLC even though long-term survival is still anticipated. CEC, as a biomarker, may be useful in predicting the efficacy of the such synergistic treatment.

Objective To evaluate the prevalence of fetal anomalies in recent 10 years and explore the effective methods of early diagnosis and treatment. Methods The clinical and pathological data of 116pregnant women who underwent induced abortion in the second trimester due to fetal anomalies 1998-2007were analyzed retrospectively. Results The fetal anomaly rate in the period 2003-2997 was 8.7%,significantly higher than that in the period 1998-2002 (3.1%,P <0.001). The diagnostic methods of fetal anomalies used during the period 2003-2007 did not show significant differences compared with those used during the period 1998-2002. The gestational week when the fetal anomalies were detected in 2002-2007 was 19.3 weeks, not significantly preceded compared with that during 1998-2002 (22.4 weeks, P > 0.05).The top 5 fetal anomalies with high incidence rates were Down' syndrome, anencephahis, lip/palate cleft, fatal cystic hygroma of the neck, and cardiac anomalies. There were 30 cases of advanced maternal age over 35 years, 60.0% of whose fetuses showed chromosome abnormality. Conclusion The incidence of fetal anomalies increases in recent years. The risk of fetal anomalies is high in the pregnant women in elder age. Pregnancy in advanced maternal age show high risk to occur fetal anomaly. Better approaches to diagnose fetal anomaly earlier are still to be found.

Objective To evaluate the intraobserver and interobserver reliability and main influencing factors of the King, Lenke, and PUMC classification systems for idiopathic scoliosis (IS) using radiographs that had not been premeasured. Methods Fifty-six patients with IS, 9 male and 47 female, aged 15.4 (11-18), underwent preoperative X-ray photography of spine standing full-length posteroanterior and lateral films and left and right supine side-bending radiographs. The films were read by four orthopedic surgeons independently to do measurement and typing according to the King, Lenke, and PUMC classification systems respectively. Two weeks later, the sequence of the X-ray films was re-ordered and the 4 surgeons read them and did measurement and typing once more. The average percentage of intraobserver and interobserver agreement was calculated. Kappa coefficients were used to determine the iutraobserver and interobserver reliability. Results King classification demonstrated fair intembserver reliability and excellent intranbserver reliability. The mean interobserver reliability was 65.8% (Kappa coefficient = 0.542), while the intraobserver reliability was 82.6% (Kappa coefficient =0.767). The main reason of disagreement was distinguishment of the King type Ⅱ and type Ⅲ. Another reason was assessment of King type Ⅴ. The complete Lenke classification demonstrated poor reliability. The mean interobserver reliability was 50.0%(Kappa coefficient = 0.438), and the intraobserver reliability was 47.0% (Kappa coefficient = 0.402).The 3 components of Lenke classification had fair interobserver and intraobserver reliability when they were examined separately. The main reasons for disagreement arose from judging whether there was a structural upper thoracic curve and assigning sagittal thoracic modifier. The PUMC type demonstrated excellent intraobserver and interubserver reliability with a mean interobserver reliability of 87.8% (Kappa coefficient =0.757), and a mean intraobserver reliability of 92.9% (Kappa coefficient = 0.958). Its subtypes demonstrated fair agreement with the mean interobserver reliability of 70.2% (Kappa coefficient =0.629), and a mean intraobserver reliability of 74.1% (Kappa coefficient =0.674). The main reasons for disagreement were definition of a curvature and Cobb angle measurement. Conclusion King and PUMC classification systems have higher interobserver and intraubserver reliability than Lenke classification. The reliability levels of the 3 classification systems are all influenced by many factors. Judgment of an upper thoracic curve, variable of Cobb angle measurement, and relationship of the central vertical sagittal line to apex of curve are the common reasons for disagreement in these 3 classifications.

Objective To investigate the role of curved planar reformation (CPR)using multi-slice spiral CT scanning to evaluate the coronal and sagittal plane in scoliosis so as to affirm the features of supine coronal and sagittal plane in idiopathic scoliosis. Methods Thoracic and lumbar spine multi-slice spiral CT scanning was undertaken on 45 patients with adolescent idiopathic scoliosis (AIS), all female, age 10-18. CPR was used to reconstruct the spine coronal and sagittai planes respectively. The values of main curve and compensate curve Cobb angles were obtained and compared with those obtained by X-ray films. Results The average CPR coronal main curve Cobb angle was smaller by 10.17° than that obtained by standing position film. The average CPR coronal compensate curve Cobb angle was smaller by 6. 97°than that obtained by standing position film. For scoliosis coronal flexibility assessment, fulcrum-bending method offered better surgical correction result than other methods. Only the post-operational Cobb angle was different between the 10-14 year-old group and 15-18 year-old group. Bending film showed differences in correction rate and flexibility rate between the main thoracic group and main lumbar/thoracolumbar group. The correction rate and flexibility rate of main curve and compensate curve of the 10-14 year-old group were significantly higher than those of the 15-18 year-old group. In the saglttal plane, the CPR thoracic kyphotic angle (T5-T12) and upper thoracic curve kyphosis of the main thoracic group were both smaller than those of the main lumbar/thoracolumbar group. The CPR thoracic kyphotic angle (T5-T12) of the 10-14 year-old group was significantly smaller than that of the 15-18 year-old group. Correlation analysis showed that the CPR main curve flexibility and bonding film flexibility were positively corrected with the surgical correction rate. Conclusion CPR using multi-slice spiral CT scanning is able to obtain multiple three-dimensional assessment by one scanning and is helpful in scoliosis curve type diagnosis and scoliosis 3D analysis.

Objective To explore the profile of ehanges of memory monitoring in patients with mild cognitive impairment(MCI). Methods Rey-auditory verbal learning test (AVLT) and feeling-of-knowing (FOK) test in episodic memory(EM) and semantic memory (SM)were conducted on 30 MCI patients, 17nudes and 13 females, aged (66±9), and 30 age, education level, word fluency test, and digit span-matched healthy persons as healthy control(HC)group. Results The EM impairment of the MCI group was (13.7±2.8), significantly higher than that of the HC group [(8.1±2.0), P <0.01], but the SM impairment of the MC1 group was (5.4±1.4), not significantly different from that of the HC group [(5.0±1.4), P >0.05]. The EM-FOK of the MCI patients with correct judgment and false recognition was (41.8±13.2)%, significantly higher than that of the HC group [(28.1±11.6)% ,P <0.01] ,however, the SM-FOK levels of the MCI patients with different judgment and recognition were all not significantly different from those of the HC group (all P > 0.05). Conclusion The MCI patients overestimate their memory performance on episodic FOK, but their SM monitoring is not impaired. The deficit of memory monitoring in MCI may be the foundation of subjective memory complaints and is useful for diagnosis of MCI. EM and SM monitedng may depend on different neural mechanisms.

Objective To study the relationship between the fulcrum bending flexibility(FBF) and correction rate (CR) of adult idiopathic scoliosis, and to explore the ability of FBF to assess the correction effect in relation to fulcrum bending flexibility. Methods 69 patients with adult idiopathic seoliosis with structural curves at thoracic or lumbarthoracic segments, 16 males and 53 females, aged 26.5(19-53) were treated by pedicle screws instrumentation. Pre-opemtive standing and fulcrum bending films and postoperative standing X-ray film were taken. Cobb angle was measured. The data underwent regression analysis with the software SPSS 10.0. Results A regression formula was established:CR =0.213 +0.768×FBF with P <0.01. Conclusion A definite linear relation exists between the FBF and CR of adult idiopathic scoliosis. By using the formula in proper samples, the effects of new instrumentation or correction technique can be objectively assessed.

Objective To investigate the immune and inflammation confusion state in severe sepsis and the effects of two way immunomodulation therapy with continuous blood purification (CBP), thymosin α1, and combined therapy of CBP and thymosin α1. Methods 91 Patients with severe sepsis aged 18,with Marshall score >5. Were randomly divided into 4 groups: CBP Group (n =22) undergoing continuous renal replacement therapy (CRRT) or molecular adsorbents recirculating system (MARS) therapy once a day for 3 days in addition to classical Surviving Sepisis Campaign (SSC) therapy, Thymosin α1 Group (n =23) undergoing subcutaneous injection of thymosin α1 1.6 mg once a day for 7 days in addition to SSC therapy, Combined Therapy Group (n = 22) undergoing CBP combined with thymosin α1 treatment in addition to SSC therapy, and SSC Group (treatment control group, n = 24) undergoing SSC therapy only. Peripheral blood samples were collected before treatment, and 3 and 7 days after the beginning of treatment (days 4 and 8) to detect the serum interleukin (IL)-6, IL-10, and tumor necrosis factor (TNF)-α. The levels of CD+ 14 monocyte human leucocyte antigen (HLA)-DR and T lymphocytes were monitored. The mechanical ventilation time, ICU stay length, and mortality within 28 d and mortality within 90 d were observed. Ten healthy persons were used as healthy control group. Results Thirty-four of the 91 patients died within 28 d with a mortality of 77.4% (Death Group) and other 57 patients were put in Survival Group. The levels of serum IL-6, IL-10, and TNFα, and IL-6/IL-10 at different time points of both Death and Survival Groups were all significantly higher, and the HLA-DR level, and CD+3, CD+4, and CD+8 T lymphocyte numbers at different time points of both Death and Survival Groups were all significantly lower than those of the healthy controls (P <0.05 or <0.01). The levels of serum IL-6, IL-6/IL-10,TNFα,HLA-DR, and CD+4, CD+4 and CD+8 T lymphocyte at different time points of Death Group were all significantly higher than those of Survival Group (P <0.05 or <0.01). The CD+3 T lymphocyte number on day 8 of Thymosin Group was significantly higher than that of SSC Group (all P < 0.05). The serum IL-6and TNFα and IL-6/IL-10 were decreased, and HLA-DR, and CD+3, CD+4, and CD+8 were increased significantly on day 8 in CBP and Combined Therapy Groups. The level of TNFot decreased, and the numbers of CD+3 and CD+4 T lymphocytes increased significantly on day 4 in Combined Therapy Group(P <0.05 or P <0.01). Compared with Thymosin Group, almost all the indexes of CBP and Combined Therapy Groups were improved, only the CD+3 T lymphocyte level on day 4 increased and the IL-6/IL-10 ratio on day 8 was decreased significantly in Combined Therapy Group (both P < 0.05). Compared with those of SSC Group, the mechanical ventilation time, length of ICU stay within 28 days, and 28 days mortality and 90 days mortality of the 3 treatment groups were all decreased, and there were statistical differences in the length of ICU stay of CBP Group and in the mechanical ventilation time and length of ICU stay within 28 days of Combined Therapy Group (both P < 0.05) . Conclusion Systemic inflammatory response and immunodeprossion exist simultaneously in severe sepsis. Thymosin α1 increases the cellular immunity, and CBP hi-modulates the immune turbulence, reduces the inflammatory mediators, and meliorates the immune homeostasis. These 2 therapies also improve the clinical prognosis and the combination of both would be more effective.

Objective To investigate the Influence of chronic cerebral hypoperfnsion on gap junction ultrastrocture and expression of connexin (Cx) 36, Cx32, and Cx26 in hippocampus the microatructure of gap junction and the expression of gap junction subunits in the hippocampus of cognition impaired animals. Cognition impairment was induced by the permanent occlusion of the carotid arteries (2-VO). Methods Twenty-seven Wistar rats underwent two-vessel occlusion (2-VO, ligation of bilateral common carotid arteries) and then were randomly divided into 2 equal groups: 2-VO 3 week, 2-VO 8 week and 2-VO 12 week groups to undergo Morris water maze test 3, 8, and 12 weeks later to observe the spatial learning and memory ability. Another 9 rats underwent sham operation to be used as control group. After Morris water maze test the rats were killed. The brains were taken out from 3 rats from each group to undergo thin-section transmission electron microscopy to observe the ultrastructure of the gap junctions in the CA1 area of hippocampus. RT-PCR and Western blotting were used to detect the mRNA and protein expression of Cx 36, Cx32, and Cx26 in the hippocampus of the other 27 rats, 6 from each group. Results Compared to the control group, the density of cytomembrane of gap junction was lower, the interspace of gap junction were larger in the 2-VO 3 week group, and these abnormalities were gradually improved in the 2-VO 8 and 12 week groups. The mRNA and protein levels of Cx36 and Cx32 of the three 2-VO groups were the lowest in the 2-VO 3 week group, and then gradually increased, however, all significanfly lower than those of the control group (all P < 0.05). The mRNA level of Cx26 was not altered significantly, while the protein expression leveh of Cx26 in the three 2-VO groups were all higher compared with the control group (all P <0.05). Conclusion The altered neuronal gap junction may participate in the cognition impairment caused by chronic cerebral hypoperfusion.

Objective To study the effect of 60Co γ ray on proliferation and differentiation potency of MSC. To evaluate the function of heart after early stage of acute myocardial infarction (AMI) in rots by transplantation of MSC. Methods Irradiated MSC was cultured with the method of adherent culture, the ability of its proliferation was detected by Methyl thiazolyl tetrazulium (MTT) shade selection test The express of Cardiac troponin T (C-TNT) and β-myosin heavy chain (β-MHC) in MSC were detected by Reverse tranacriptase-polymerase chain reaction (RT-PCR), which was induced by 5-azacytidine. Using a rat model of AMI , irradiated and unirradiated MSC were transplanted into myocardium after infarction. At 4 weeks, the cardiac dimension and functions were assessed by echocardiography and hcmodynamics, the microvessel density were detected after Ⅷ factor immunohistochemistry stalin Results The ability of proliferation in 4Gy group was depressed. C-TNT and β-MHC expressed somewhat in unirradiated group. When compared with the control group (6.6±0.6), the microvessel density in both transplanted groups (12.7±2.2, 11.5±2.1) increased, contributing to improvement in cardiac function and dimension. Conclusion MSC with or without differentiation potency can improve cardiac function equally at the early stage of AML.

The regeneration and repair of central nervous system after injury has always been a difficult problem in the scientific circle, and the current research has not made a breakthrough with clinical significance [1]. With the discovery of adult neural stem cells, the development of material technology and cell culture technology, tissue engineering methods have brought hope for the treatment of nerve injury [2-3]. The tissue engineering cell scaffold materials must first have good biocompatibility. This experiment explored the biocompatibility of collagen-heparin sulfate bioscaffold implanted in pig brain.

A 27-year-old female was admitted to hospital for 31+4 weeks of menopause, abdominal mass and abdominal pain were found for 5 months. The patient underwent exploratory laparotomy + left adnexectomy + right ovarian cyst excision + partial omentectomy on 22 August 2005 due to "double ovarian cyst". The pathological diagnosis was poorly differentiated mucinous papillary cystadenocarcinoma of the left ovary and mature teratoma of the right ovary.