Volume 88  ·   Issue 42  ·  2008

According to the annual report of cardiovascular diseases in China in 2006, the number of patients with hypertension in China has reached 200 million [1], but the control rate of hypertension is still at a low level. In the past few decades, the treatment of hypertension has changed from simple blood pressure control to comprehensive control of risk factors, and the choice of therapeutic drugs has transitioned from single drug therapy to combination therapy. The blood pressure target has been reduced from the blood pressure in 1970s to 160/95 mm Hg, to the blood pressure should be lower than 140/90 mill Hg, and then evidence-based medicine has confirmed that the blood pressure of high-risk hypertensive patients should be lower than 130/80 mm Hg [2].

Nowadays, the progress of medical science and social development make medical education face severe challenges, and the national demand for high-level medical talents is increasing, and the demand for medical talents is also increasing. In order to train medical talents with generous knowledge base of humanities, social sciences and natural sciences and solid medical theoretical knowledge, some medical colleges in China have started to run 8-year medical education on a trial basis [1].

Objective To investigate the prevalence, rates of awareness, treatment, and control,and risk factors of hypertension among the residents in Harbin, Heilongjiang Province. Methods 5523 residents, 2325 males and 3198 females, aged 15 and over, underwent questionnaire survey to understand their general conditions, history of present disease, awareness of disease, treatment, working condition, etc, and measurement of blood pressure (BP), height, body mass, and serum glucose and triglyceride. Results The no-standardized prevalence of hypertension was 31.30% in general(1674/5348), 38.52% in the males (829/2152), and 26.44% in the females(845/3196). The corresponding standardized prevalence rates of hypertension were 24.91%, 37.72%, and 20.75% respectively. The rates of awareness, treatment, and control of hypertension were 57.41%, 32.74%, and 5.85% respectively. Multiple logistic regression analysis revealed that employment condition, family history of hypertension, alcohol consumption, body mass index, level of triglyceride and serum glucose were risk factors of hypertension. Conclusion Hypertension is highly prevalent is highly prevalent and the rates of awareness, treatment, and control of hypertension are still rather low in Harbin.

Objective To evaluate the curative effects of slow releasing feiodipine on mild to moderate primary hypertension and its influence on the pulse wave velocity of slow of the patients. Methods 260 patients with mild to moderate primary hypertension, aged 35-79, received slow releasing felodipine with the initial dosage of 5 nag once daily for 2 weeks. By the end of the second week, 222 of the 260 patients with their blood pressure (BP) levels < 140/90 mm Hg were treated by the same regimen for 12 weeks. Pulse wave velocity (PWV) was measured before the treatment, and by the ends of the second and fourteenth weeks. Intention to treat analysis (ITT analysis) was adopted. Results After 2-week treatment the systolic BP decreased by 21.4 mm Hg and diastolic BP by 14.2 mm Hg ( both P<0.01). After14-week treatment, the BP decreased by 24.8/17.5 mm Hg (P<0.01), the systolic BP decreased by 16.5% and the diastolic BP decreased by 18.4%. The BP of the patients with their BP levels up to standard decreased by 22.8/ 15.1 mm Hg (P<0.01) by the end of the second week and decreased by 25.0/17.9 mm Hg by the end of the 14th weeks. The PWV of all patients decreased by 0.58 m/s after 2-week treatment (P<0.05)and by 0.86 m/s after 14-week treatment (P<0.05 ). The PWV of those up to standard decreased by 0.55 m/s after 2-week treatment(P<0.05) and by O. 86 m/s after 14-week treatment (P<0.05). Adverse events were observed in 56 patients (21.5%), including headache, flush, and dizziness, however, no severe adverse event was found. No significant changes in laboratory examinations and ECG were found after treatment. Conclusion Slow releasing felodipine is effective in treating mild to moderate primary hypertension, with sustained efficacy of lowering blood pressanre and PWV.

Objective To investigate the effect of baseline homocysteine (Hey) level on the efficacy of enalapril maleate and folic acid tablet in lowering blood pressure and plasma Hey in patients with mild or moderate essential hypertension. Methods 456 patients with mild or moderate essential hypertension were from 7 hospitals in Southern and Northern China, 196 males and 260 females, aged 18-75, were randomly assigned to one of the 3 groups: Group 1 treated with enalapril 10 mg (n=153) ; Group 2 treated with enalapril maleate and folie acid tablet at the ratio of 10/0.4 (n=151) ; and Group 3 treated with nalapril maleate and folic acid tablet at the ratio of 10/0.8 (n=152). Blood pressure was measureded every 2 weeks and plasma Hcy level was measured before the experiment, 4 weeks after the beginning of experiment, and by the end of experiment. Results Compared with the baseline levels, the blood pressures of the 3 groups were all well controlled (all P<0.01). There was no significant difference among the 3 groups in the efficacy in lowering blood pressure, even after stratification by the baseline Hey level. 75% of all the subjects had elevated plasma Hey level (≥10 μmoi/L). The blood pressure and Hey lowering rates of the subjects with hyperhomecysteinemia in Groups 2 and 3 were 70.9% and 67.0% respectively, both significantly higher than that of Group 1 [ 45.6%, OR (95% CI) : 3.0 (1.7-5.5), P=0.000 and OR= 3.3 (1.8-5.9), P=0.000] , and in lowering Hcy [OR(95%CI) : 7.5(2.6-21.2), P=0.000 and 3.5 (1.4-8.7), P=0. 007 ] subjects with hyperhomocy steinemia. The Hcy lowering efficacy in the patients without hyperhomocysteinemia of Group 3 was significantly higher than that of Group 1 ( P=0.016). Conclusion Hyperhomocysteinemia is s extremely common in Chinese hypertensive patients. Enalapril maleate combine with folic acid tablet shows better efficacy in lowering either blood pressure or Hcy in hypertensive patients with hyperhomocysteinemia.

Objective To summarize the clinical features of osteonecrosis of the jaw (ONJ) in multiple myeloma (MM) patients treated with bisphosphonate. Methods The clinical data of 4 patients with bisphosphonate-related (ONJ) in MM were reported and literatures were reviewed. Results In these patients , 3 males and 1 female, aged 59-73, pamidronate combined with chemotherapy, high dose glucocorticesteroid, and anti-angiogenic drug had been used for 12-44 months before the occurrence of ONJ. In treatment of ONJ conservative debridement of necrotic bone, systematic use of antibiotics, use of chlorhexidine acetate gargle, and withdrawal of bisphosphonates were preferable to aggressive surgical measures. Conclusion Long-term use of bisphosphonates combined with chemotherapy in MM may cause ONJ that involves both mandible and maxilla. No satisfactory therapy is currently available.

Objective To investigate the relationship between vascular endothelial growth factor C (VEGF-C) expression level and lymph node metastasis in non small cell lung cancer (NSCLC). Methods Fifty-two NSCLC patients, 38 males and 14 females, aged (59±11) (29-77), were divided into 2 groups based on the pathological examination: lymph node metastasis positive group (n=25) and lymph node metastasis negative group (n=27). RT-PCR and immtmohistochemistry were used to detect the mRNA and protein expression of VEGF-C in the tumor tissues and lymph nodes resectod during operation. Reeults The VEGF-C mRNA expression level in the lung tumor tissue of the lymph node metastasis positive group was 0.273±0.179, significantly higher than that in the lymph node metastasis negative group (0.089± 0.087, P<0.01). The VEGF-C mRNA expression level in the positive lymph node of the lymph node metastasis positive group was 0.207±0.174, significantly higher than that in the lymph node metastasis negative group (0.114±0.107, P<0.01), but not significantly different from that in the negative lymph nodes of the same group(0.196±0.186, P>0.05). The VEGF-C protein positive rate of the lung cancer tissues of the lymph node metastasis positive group was 93.3% ( 14/15 ), significantly higher than that of the lymph node metastasis negative group (6.7%, 1/15, P<0.01). The VEGF-C protein positive rate of the metastasis positive lymph noses was 80.4% (37/46), and all 52 metastasis negative lymph nodes were VEGF-C protein negative. Conclusion VEGF-C mBNA and protein expression levels predict lymph node metastasis and can be useful predictors of lymph node metastasis in NSCLC.

Objective To investigate the relationship between the blood glucose variability and microalbuminuria (MAU) in type 2 diabetic patients with well-controlled glycosylated hemoglobin (HbAlc) and the influencing factors of blood glucose variability. Methods One hundred and seventy-six type 2 diabetic patients with HbAlc under 6.5% and 48 subjects with normal glucose regulation were monitored using the continuous glucose monitoring system (CGMS). The mean blood glucose (MBG) and mean amplitude of glucose excursions(MAGE) were analyzed. Results ( 1 ) The MBG and MAGE levels of type 2 diabetic patients were (7.0±0.9) and (3.8±2.5) mmol/L respectively, both higher than those of the subjects with normal glucose regulation [ (5.4±0.6) and (2.0±0.7) mmol/L respectively, both P< 0.01]. (2)The incidence ratio of MAU of the patients with ascended SAGE level was 18.7%, significantly higher of those with normal MAGE (7.1%, P<0.05). (3) The SAGE level was positively correlated with age, duration of diabetes, and systolic blood pressure, and negatively correlated with glomerular fdtration rate and the levels of fasting and postprandial C-peptide. Multivariant regression analyses indicated that duration of diabetes and the level of postprandial C-peptide 30 min after meal were the independent influential factors of MAGE. (4) In the type 2 diabetic patients, the MAGE of the MAU group was higher than that of the non-MAU group (P<0.05). Logistic regression analyses indicated that diastolic blood pressure and MAGE were the risk factors of MAU (OR=1.201 and 1.357, both P<0.05). Conclusion In weB-controlled patients with type 2 diabetes, blood glucose variability is one of the risk factors for MAU, duration of diabetes and early stage of insulin secretion function are the main factors influencing glycemic variability.

Objective To evaluate the diagnostic accuracy of anti-α-fodrin antibody for Sjogren's syndrome (SS). Methods Qualified literatures on evaluation of anti-ot-fodrin antibody in diagnosis of SS in English and Chinese published between January 1997 and December 2007 were retrieved from the Cocharane Library, Medline, Embase, and China National Knowledge Infrastructure (CNKI) databases, etc. Two reviewers independently assessed the methodological quality of each study with the tool QUADAS ( quality assessment of diagnostic accuracy studies). Statistical analysis was performed by employing MATLAB, Review Manager 4.2 and Meta-Discl. 4. A reefs-analysis of the reported sensitivity and specificity of each study and Summary Receiver Operating Characteristic (SROC) curve was performed. Results Eighteen literatures were included at last. After testing the heterogeneity of the included articles, proper effect model was selected to calculate the pooled weighted sensitivity and specificity with 95% confidence interval: for anti-α-fodrin antibody IgG, the sensitivity was 0.40 [95% CI(0.37-0.43)] and the specificity was 0.82 [95% CI(0.79-0.84)], the area under the curve (AUC) of SROC was 0.8029 (SE=0.0580). Eight studies tested anti-α-fodrin antibody lgA, the pooled weighted sensitivity and specificity with 95% confidence interval were 0.34 [95% CI(0.30-0.38) ] and 0. 83 [95% CI(0.79-0.86) ] respectively, the AUC of SROC was 0.6374 (SE=0.1841), the synthesis data all showed heterogeneity. The subgroups were analyzed to identify the sources of heterogeneity according to age, race, assay method, agent source, diagnostic criteria, and country. There was homogenicity among the 4 studies from China, and the 6 studiesfrom Japan,the AUC of SROC were 0.7343 (SE=0.0448) and 0. 9273 (SE=0.0394), respectively. Conclusion Diagnostic criteria, agent source, assay method, age, race, and country are the important sources of heterogeneity. Anti-a-fedrin antibodies IgG and IgA have relatively low pooled sensitivity and relatively high pooled specificity. Negative anti-a-fodrin antibody has not important value in excluding SS, but positive anti-α-fodrin antibody may be a useful parameter in clinical diagnosis of SS.

Objective To analyze the antibiotic resistance of the Pseudomonas aeruginosa (PA) isolated from pediatric patients and the rosistant genes of beta-lactam antibiotics thereof. Methods 146 PA strains were isolated from pediatric patients. Agar dilution method recommended by the Clinical and Laboratory Standards Institute was used to examine the minimum inhibitory concentrations (MICa) of 12 antimicrobial agents, including the penicillins, third and fourth genet ration cephaloapofins, carbapenemases, Aztreonam, β-lactamase inhibitors, quinolones, and aminoglycosidea. PCR was used to detect the expression of the genes TEM, SHV, OXA, PER, GES, CTX-M,IMP, VIM,DHA, MIR, FOX, and oprD2. Results The multi-drug resistance rotes against different antibiotic were high among the 146 PA strains. The rates of imipenem and meropenem resistance were 41.1% and 35.6% respectively. Among the 146 PA strains, 46 (31.5%) were positive for the MBL genotype; 38 (82.6%) carried the blaIMP gene, 8 (17.4%) carried the blaVIM gene, and 114 (78.1%) were oprD2 negative. The genes TEM, SHV, OXA, CTX-M, PER, VEB, GES, FOX, MIR, and DHA were not found in all strains. Conclusion Many PA isolated from pediatric patients carry the genes IMP or VIM and losses oprD2 gene related to the expression of the outer membrane porin OprD2. The loss of the gene oprD2 is essential mechanism of beta-lactam antibiotics resistance in PA.

Objective To investigate the effects of adenovirus melanoma differentiation associated gene-7 ( mda-7 )/IL-24 on the growth and apoptosis of human breast cancer cells. Methods Human breast cancer cells of the lines MCF-7 and MD-MBA-453 were cultured and transfected with purified adenovims mda-7/IL-24. 48 h later, Western blotting was used to detect the protein expression of mda-7/IL-24 in the MCF-7 ceils. The growth of Ad-mda-7/IL-24 in the MCF-7 cells was assayed by crystal violet staining and MTr method. The apeptotic effect of Ad-mda-7/IL-24 in the MCF-7 and MDA-MB-453 cells was detected by Hoechst staining and annexin V method. Nude mice were inoculated with MCF-7 cells and randomly divided into 2 groups to be injecte4d with Ad-mda-7/IL-24 of PBS. The size of tumor was observed. Results Western blotting showed that mda-7/IL-24 was expressed in the transfected MCF-7 cells. Crystal violet staining and MTY method showed that Ad-mda-7/IL-24 dose and time-dependently inhibited the growth of the MCF-7 and MDA-MB-453 cells. Hochest staining and annexin V test indicated obvious apoptosis of the breast cancer cells. The size of tumor of the nude mice injected with Ad-mda-7/IL-24 was significantly smaller than that of the control mice. Conclusion Ad-mda-7/IL-24 effectively inhibits the growth of breast cancer cells and induces its apoptosis.

Objective To investigate the influence of Chinese medicine Huishen granule (HG) containing ginseng, grassleaved sweetlag rhizome, pilose deer antler, etc, on learning and memory functions in diffuse axonal injury (DAI) and the mechanisms thereof. Methods Impact acceleration method was used to establish DAI Wistar rat models. Twenty model rats were randomly divided into 2 equal groups, the DAI + HG group treated with gastric perfusion of HG 3 times a clay since 24 h after the establishment of model for 14 days, and the DAI group without treatment. Ten rats underwent sham operation as controls. Fourteen days after the injury, Morris water maze (MWM) test was used to detect the rat's abilities of learning and memory for continuous 5 days. The changes of escape latency in acquisition of the task, the percentage of time spent in target quadrant, and the number of crossing the point of original platform in probe test were recorded. At day 20 after the-operation, the rats were subjected to long-term potentiation (LTP) recording in hippocampns to measure the percentage of slope and baseline of excitatory post-synaptic potential (EPSP). Two rats from each group were killed 24 h, 14 d, and 20 d after the operation with their brains taken out, HE and immunohistoehemical staining were employed to exam the brain lesion at 24 h, day 14 and 20 post-injury. Results The escape latency of the DAI group was ( 32.8±4.6) s , significantly longer than those of the DAI +HG and sham operation groups [(20.3±0.7) and (16.8±0.8)s respectively, both P<0.05]. The target quadrant staying time percentage and number of platform location crossings of the DAI group were (36.4±3.2)% and 4.5±0.6 respectively, beth signifieantly less than those of the DAI+ HG and sham operation groups [ (46.0±2.4) % and 6.8±0.8, and (46.9±2.1) % and 8.1±0.8 respectively,all P<0.05]. The LTP level of the DAI group was (101.4±3.3)%, significantly lower than those of the DAI + HG and sham operation groups [(116.3±6.7)% and (117.9±2.8)% respectively, both P<0.05 ]. No significant differences in the parameters were found between the DAI + HG and sham operation groups ( all P>0.05 ). Classical pathological changes of DAI occurred in the brains of the DAI and DAI + HG groups at the time point of 24 h, and mitigated partly at the time points of day 14 and 20. Conclusion The learning and memory impairment of DAI was ameliorated significantly with the treatment of Chinese medicine HG, owing to the recuperation of synaptie plasticity in hippocampal area.

Objective To explore the effects of acute and chronic routine cytomegalovirus (MCMV) infections on the regulatory T cells (Treg) ratio and protein expression of the Th1/Th2 transcription factors T-bet/GATA-3. Methods 120 BALB/c mice were randomly divided into 2 equal groups: MCMV-infected group undergoing infra-peritoneal injection of homogenate of salivary gland containing MCMV, and mock infection group undergoing infra-psritoneal injection of normal homogenate of salivary gland 1, 3, 7, 14, 28, 45, 60, 75, 90, and 120 days after infection 6 mice from each group were killed to examine the viral load of the heart, lung, liver, and kidney by plaque assay to access the status of MCMV infection. Suspension of splenocytes was prepared. The proportion of CD4+CIY25+Foxp3+Treg in the splenocytes was measured by flow cytometry. Western blotting was used to detect the protein expression of T-bet/GATA-3. Results The cutoff point between acute and chronic points was the 28th day. The CD4+ CIY25+Foxp3+Treg proportion in splenecytes significantly decreased during the acute infection stage and to the lowest level of (1.46±0.27) % at day 28, significantly lower than that of the mock infection group [ (2.78±0.29 ) %, P<0.05 ] ; then obviously increased in the chronic infection stage, increased to (4.51±0.24)% at day 60, significantly higher than that of the mock infection group [ (2.69±0.12)%, P<0.05], and continued to increase still. The protein level (K value) of T-bet of the MCMV infection group peaked to the level of (0.618±0.053) on day 3, obviously higher than that of the mock infected group [ (0.205±0.026) ], then decreased to the level similar to that of the mock infection group on day 28, and was obviously lower than that of the mock infection group on day 75. Whereas the protein level of GATA-3 of the MCMV group increased to (0.836±0.061 ) on day 3, markedly higher than that of the mock infection group (0.398±0.022), peaked on day 7, then gradually decreased, and remained at the levels similar to those of the mock infection group from day 75 to day 120. Conclusion In the acute infection stage, MCMV up-regulates the T-bet and GATA-3 protein expression. But during the chronic infection stage, MCMV induces a marked proliferation and activation of Treg cells which further inhibit the Th1 and Th2 reactions, especially Th1 response. Treg proliferation may be an important mechanism of chronic and persistent CMV infection in the host.

Objective To isolate and identify the side population (SP) cells in pancreatic cancer and investigate the role of phosphatidylinositol 3-kinase/mammalian target of rapamycin (PI3K/mTOR) pathway in the survival and proliferation of them. Methods Pancreatic cancer cell of the lines PANC-1, BXPC-3, ASPC-1, PC-3, aad SW-1990 were cultured. Hoechst 33432 staining and fluorescence-activated cell sorter (FACS) were used to sort the SP cells. Verapamil, inhibitor of ATP binding cassette (ABC) transporter, was added before the Hoechst 33342 inoculation to observe its influence on the SP proportion. Media with LY294002, specific inhibitor of PI3K, or rapamycin, specific inhibitor of mammalian target of rapamycin (roTOR), were used to culture PANC-1 cells to observe the survival of cells. Twenty-one NOD-SCID mice were randomly divided into 7 equal groups. Four groups were inoculated subcutaneously with SP cells of the concentrations of 5×105, 5×104, 5×103, or 1×103 at the right axillary fossa and with non-SP cells at the left then Hcechst 33342 staining, flow cytometrie sorting were used to detect the content of SP cells at the left axillary fossa The other 3 groups were injected subcutaneously with non-Hoechst 33342 treated cells of the concentrations of 5×105, 5×104, 5×103, or 1×103 at the right axillary fossa and PBS at the left axillary fossa. Ten weeks later the mice were killed to undergo pathological examination. Results All cell lines were found to exhibit verapamil-sensitive SP cells except BXPC-3 cells. The SP cell proportion of the PANC-1 cells was 7.84%. No SP cell was found in the cells treated with verapamil. The colonyformation ability of the SP cells was (43.7±3.1) %, significantly higher than those of the non-SP cells and cells without Hcechst 33342 cells [(8.3±1.6)% and (10.2±1.9)% respectively, both P=0.000]. The tumorigenic ability of the SP cells was 100 times as those of the non-SP cells and Hoechst 33342 untreated cells. After addition of LY294002 and rapamycin the fractions of the SP cells in the in PANC-1 cells decreased from (7.60±0.27)% to (1.90±0.22)% and (1.14±0.20)% respectively, both P=0.000, and they preferentially inhibited the SP cells rather than non-SP cells. Conclusion SP cells are enriched in pancreatic cancer stem-like cells. PI3K/mTOR pathway is critical for pancreatic SP cells maintenance that can be selected as a new target for inhibiting cancer stem-like cells.

Objective To examine the effects of YM976, a phosphodiesterase (PDE)4 inhibitor, on mucin hypersecretian of airway stimulated with acrolein. Methods Forty-eight SD rat were randomly divided into 6 equal groups. Twenty-four rats underwent gastric perfusion of YM976 0.5, 1.5, or 4.5 nag· kg-1·d-1(n=8 for each group) and then underwent aerosol inhalation of 3.0 ppm acrolein 6 hours per day for 12 days so as to establish rat models of airway mucus hypersecretion. Eight rats underwent aerosol inhalation of 3.0 ppm acrolein only for 12 days to establish rat models of airway mucus hypersecretion without any drug intervention (aerolein model group). Eight rars underwent gastric perfusion of 5% methylcellulose and then aerolein inhalation for 12 days ( methyleellulose group), and another 8 rats underwent gastric perfusiun of YM976 4.5 mg/kg once a day and then inhalation of normal saline for 12 days ( YM976 control group), On the 13th day the rats were killed. Bronchoalveolar lavage fluid (BALF) was acquired for cell count, and ELISA was used to detect the levels of tumor necrosis factor (TNF)-α and cytokine-induced neutrophil chemoattractant (CINC)-1 in the BALF. The mucin level in the airway mucous membrane was detected by alcian blue (AB)/periodic acid-Schiff(PAS) method. Muc5ac protein was measured by immunohistochemical staining and Western-blotting, and Muc5ac mRNA was detected by BT-PCR. Results The percentages of positive AB/PAS staining of the acrotein model group and the 3 YM976 groups (0.5,1. 5,and4.5 mg·kg-1·d-1) were 23.65±2.86, 22.63±2.12, 16.34±1.72, and 5.03±0.72 respectively. The integral optical density (IOD) levels of Muc5ac inanunohistochernical staining of the methylcellulose group and the 3 YM976 groups were 0.54±0.05, 0.49±0.06, 0.32±0.04, and 0.22± 0.04 respectively. The IOD of Muc5ac protein expression by Western-blotting of the methylcellulose group and the 3 YM976 groups were 1.177±0.190, 0.806±0.180, 0.303±0.061, and 0.134±0.035 respectively. The IOD of Mue5ac mRNA expression by RT-PCR of the methylcellulose group and the 3 YM976 groups were 0.246±0.021,0.223±0.020, 0.161±0.012, and 0.097±0.011 respectively. The TNF-α and CINC-1 levels in the BALF of the methylcellulose group and the 3 YM976 groups were (96.77± 2.31), (87.65±2.75), (73.56±2.62), and (52.23±2.79) μg/L respectively, and (145.75± 4.43), (139.73±5.51), (95.34±5.13), and ( 65.74±5.62) μg/L respectively, all significantly higher than those of the normal control group [4.38±0.32, 0.12±0.02, 0.058±0.024, 0.061±0.006, (18.23±2.32) μg/L and (33.56±3.43) μg/L respectively,all P<0.05], but those of the YM976 1.5 mg·kg-1·d-1 and 4.5 nag·kg-1·d-1groups being significantly lower than those of the methylcellulose group (both P<0.05). Conclusion YM976 inhibits airway inflammatory response and airway mucin hyperseeretion induced by acrolein.

The patient was a 71-year-old female who was diagnosed with essential thrombocythemia (ET) for 10 years and had increased white blood cells for 1 month. She was re-admitted to the hospital on January 9, 2008. The patient had bleeding after tooth extraction in August 1998. At that time, the blood routine examination: white blood cells 6.2×109/L, hemoglobin 127 g/L and platelets 1300×109/L was performed. She was admitted to the Department of Hematology of the PLA General Hospital and diagnosed with ET by bone marrow examination. She was treated with hydroxyurea, and the platelets were close to normal after being discharged.

With the development of cell and molecular biology, the research of biomechanics has been deepened from the level of organs and tissues to the level of cells, molecules and genes, resulting in a new interdisciplinary subject-mechanical biology. Different from biomechanics, mechanical biology has shifted its research focus from mechanics to biology, studying how mechanical stress regulates the morphology and structure of tissues. More and more studies have shown that mechanical stress can regulate many functions of cells, such as cell proliferation, gene expression and protein secretion [1].

the clinical trial of SEAS (the Simvastatin and Ezetimibe in Aortic Stenosis), in which Simvastatin and Ezetimibe were combined in patients with Aortic Stenosis, showed that the incidence of cardiovascular events decreased after 5 years of treatment, but the incidence of tumor in the experimental group with Ezetimibe was higher than that in the control group.

The laboratory department is a highly dangerous workplace, and the hazards caused by safety are far more widespread than the hazards caused by inspection quality. The hazards caused by SARS in 2003 are equal to five "9.11" terrorist events. It can be seen that the safety management of the laboratory department is a hundred secrets. Our country promulgated the<Regulations on Safety Management of Pathogenic Microorganism Laboratories>, which stipulates the purpose of protecting laboratory personnel and public health in pathogenic microorganism laboratory activities, so that the management of pathogenic microorganism laboratories in China enters the track of legal management.

Case 1 male, 12 years old, was admitted to our hospital in August 1994 due to repeated fever for 13 months. The child had irregular fever, usually above 40℃, no fear of cold and chills, and had been admitted to other hospitals many times without confirmation of diagnosis. There were no clear abnormalities in physical examination, no abnormalities in chest X-ray, blood test, urine and fecal routine, liver function, anti-streptococcal hemolysin "O" (ASO), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), Feida reaction and cold agglutination test, and blood and urine bacterial culture were negative.