Volume 88  ·   Issue 22  ·  2008

In 1982, Australian pathologist Warren isolated a curved or S-shaped bacterium called Helicobacter pylori (Hp) from gastroscopic biopsy specimens of patients with chronic gastritis and peptic ulcer. After intensive research, Warren and his colleague Marshall found that these bacteria are closely related to gastric mucosal inflammation.<The discovery of Hp is a major medical event and a milestone in the re-understanding of many clinical diseases, especially upper gastrointestinal diseases. Therefore, the discoverers of Hp, Warren and Marshall, won the 2005 Nobel Prize in Physiology or Medicine.

Neonatal hearing screening includes screening, diagnosis, intervention, follow-up and rehabilitation. It is a socialized and systematic eugenic project. Its aim is to make infants and young children with hearing loss "early detection, early diagnosis, early intervention and rehabilitation", and the ultimate aim is to make them speak and return to the mainstream society.

Objective To investigate the effects of tumor necrosis factor-α-inducing protein-α (Tipot) released from Helicobacter priori (Hp) on human gastric epithelial cells.Methods Human gastric epithelial cells of the line GES-1 were cultured.Tipa gene located in Hp 0596 was extracted from the genome sequence of the Hp strain 26695 and its open reading frame were cloned into the eukaryotic expressing vector pcDNA3.1.The recombinant plasmid pcDNA3.1-Tipα thus obtained and the blank plasmid pcDNA3.1 were transfected into the GES-1 cells,and the G418-resistent clones were screened.The GES-1 cells transfected with blank vector pcDNA3.1 and normal GES.1 cells were used as controls.RT-PCR and Western blotting were used to detect the expression of Tipa.The influences of Tipa protein on the cells proliferation,apoptosis and cell cycles,concentration of related cytokines such as TNF-α,IL-1βB,and IL-8,and the expression of Bcl-2 and P53 genes were respectively observed by MTT assay,flow cytometry,ELISA,and Western blotting.Results ①A eukaryotic expression vector of Hp Tipa was successfully constructed.Steadily transfected strains were screened by G418.②MTT method showed that the growth curve of the GES-1 cells transfected with GpcDNA3.1-Tipetwas hisher,showing a faster growth.③Flow cytometry showed an inerease in the proportion of the S-phase and a decrease in the G1-phase in the GpcDNA3.1-Tipα-transfected cells (45.33±1.03)% vs (38.24±1.25)%、(33.94±1.67)%,(41.39±0.08)% vs (49.74±0.12)%、(49.27±0.15)%],and the apoptotic rate of the GpcDNA3.1-Tipa-transfected cells was 0.76±0.04,significantly lower than those of the GPeDNA3.1-transfected cells and non-transfected cells(16.84±2.16 and 8.36±1.07 respectively,both P<0.05).④EUSA showed that there was no significant difference in intracellular TNF-a concentration among the 3 GES-l cell groups (all P>0.05),however,the extra-cellular TNF-α level of the GpcDNA3.1-αTipa.transfected cells was significantly hisher than tllose of the 2 control groups (both P<0.05),and that the intra-and extra-cellular IL-1β and IL-8 concentrations of the GpcDNA3.1-Tipα-transfected cells were both significantly higher than those of the 2 control groups (all P<0.05).⑤The expression level of Bcl-2 gene of the GpcDNA3.1-Tipac-transfected cells was obfiously hisher than those of the 2 control groups,and the expression of P53 gene of the GpcDNA3.1-Tipαc-transflected cells was lower than those of the 2 control cells.Conclusion The GpcDNA3.1-Tipα-transfected cells steadily and highly express Tipα protein,that induces the high expression of TNF-α,IL-1β,and IL-8,proinflammatory cytokines,enhances cell proliferation,and upregulates Bcl-2 gene and down-regulates P53 gene.Tipα may play an important carcinogenic role in gastric cancer progression.

Objective To investigate the expression of connexin (Cx) 32 and Cx43 genes in gastric cancer and precancerous lesion,and to investigate the relation between the changes of expression of Cx32 and Cx43 genes and Helicobacter pylori (Hp) infection.Methods Gastroscopy and biopsy of gastric mucosa were conducted on 33 patients with chronic superficial gastritis(CSG),88 with precancerous lesion,and 70 with gastric cancer.Hp was detected by rapid urease test,basic fuchsin staining,and 14C-urea breath test.The CagA gene of Hp Was determined by PCR.SABC immunohistochemical method was used to detect the expression of Cx32 and Cx43 genes in gastric mucosa biopsy specimens.Results The positive expression rates of Cx32 and Cx43 genes were 15.7%and 32.9%respectively in the gastric cancer patients,51.1% and 54.5%in the patients with precancerous lesion,and 100.0%and 93.9%in the CSG patients.The positive Cx32 and Cx43 expression rates of the gastric cancer and precancerous lesion patients were significantly lower than those of the CSG patients(all P<0.05).The positive Cx32 expression rate of the gastric cancer patients with Hp infection was 16.7%,not significantly different from that of the gastric cancer patients without Hp infection(13.6%).The positive Cx43 expression rate of the gastric cancer patients with Hp infection was 25%,significantly lower than that of the gastric cancer patients without Hp infection(50%,P=0.039).The positive Cx32 and Cx43 expression rates and expression intensity of the precancerous lesion patients with Hp infection were all significantly lower than those of the precancerous lesion patients without Hp infection(all P<0.05).The positive Cx43 expression rate of the gastric cancer patients with CasA+ Hp infection was 17.9%,significantly lower than that of the CasA- Hp group (55.6%,P=0.027),however,the positive Cx32 expression rate of the gastric cancer patients with CasA+Hp infection was 12.8%,not significantly different from that of the gastric cancer patients with CagA- Hp infection(33.3%,P=0.159).The positive Cx32 and Cx43 expression rates of the CSG patients with CagA+ Hp and CagA- Hp infection were all 100%,but the expression intensity of the CagA+ Hp group was significantly lower than that of the CagA- Hp group(P=0.032).The positive Cx32 and Cx43 expression rates dfter Hp eradication of the precancerous lesion patients with Hp infection were 97.9% and 91.7% respectively,both significantly higher than those before eradication therapy(41.4% and 44.8% respectively,both P<0.05).However,the positive Cx32 and Cx43 expression rates of the precancerous lesion patients with Hp infection without Hp eradication were still 40%and 50%,not significantly different from those before treatment.Conclusion The expression levels of Cx32 and Cx43 in gastric cancer and precancerous lesion decrease.The change of expression of Cx43 was associated with Hp infection,especially CageA+ Hp infection.Hp eradication in patients with precancerous lesion up-regulates the expression of Cx32 and Cx43.

Objective To establish a model of long-term infection with Helicobacter pylori (Hp) in Mongolian gerbil (Meriones unguiculatus),and to investigate if Hp combined with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) has a synergistic effect to induce gastric mucosa injury.To investigate pathological changes of gastric mucosa during long-term Hp infection in Mongolian gerbil model.Methods 90 healthy male Mongolian gerbils were randomly divided into 4 groups:Hp group (n=24) undergoing gastric perfusion of Hp suspension of the line NCTC11637 in brain-heart infusion(BHI)108-109CFU/ml once a day for 10 days and then gastric perfusion of 1 ml normal saline (NS) once a day for 10 days since the 4th week after Hp perfusion.Hp+MNNG group (n=24) undergoing gastric perfusion of Hp solution once a day for 10 days and then MNNG l ml(2 ms/ml) once a day for 10 days,MNNG group (n=20) undergoing gastric perfusion of BHI once a day for 10 days and then gastric perfusion ofMNNC once a day for 10 day since the 4th week after BHI perfusion,and control group (n=22) undergoing gastric perfusion of BHI once a day for 10 days and then gastric perfusion of NS again once a day for 10 day since the 4th week after the BHI peffusion.4 and 8 weeks 1 gerbil from the control group and 2 gerbils from the Hp and Hp+MNNG groups each were killed to observe the pathological changes and Hp eolonization by liquid-based urease test and Warthin-Starry silver staining.20 and 40 weeks after the Hp inoculation 10 gerbils from each group were killed to observe the pathology of the gastric mucosa.Results (1) A Mongolian gerbil model of long-term Hp infection Was successfully established.(2)Hp induced the process progressing from normalgastric mucosa→chronic atrophic gastritis→intestinal memplasia→ dysplasia.Until 40 weeks after Hp infection,the gastric mucosa of the control group remained normal.Twenty weeks after Hp infection 3 gerbils in the Hp group and 1 gerbil in the Hp+MNNC group showed glandular atrophy and intestinal metaplasia respectively,and 40 weeks after infection,glandular atrophy,intestinal memplasia,and dysplasia at different degrees in the gastric mucosa were seen in the three experimental groups.The pathological changes of the Hp+MNNG group were the most severe.The incidence rates of precancerous lesions of the Hp+MNNG group were significantly higher than those of the other groups,but no gastric carcinoma was found in the experimental animals.Conclusion Hp colonizes stably in the glandular gastric mucosa of Mongolian gerbils.The histological changes after infection are similar to those of the Hp infected human being.Hp and MNNG botll cause the injury of gastric mucosa.With synergistic effect.the two pathogenic agents attack the gastric mucosa,they cause more severe injury.

Objective To evaluate the quality of life after cochlear implantation in adult cochlear implant recipients and to assess the applicability of Nijmegem Cochlear implant Questionnaire (NCIQ).Methods Thirty-two postlingually deaf patients,19 males and 13 females,underwent cochlear implantation with the age at operation of 35.27 (18-66) during the period from 1996 to the end of 2006.NCIQ was used to investigate the application of the equipment,hearing ability,and emotional exchange ability.Results All of the patients used their cochlear implants for 16 h every day on average with an exDerience in using such implant of more than 6 months.Improvement was seen in sound recognition,speech recognition,confidence,daily life,social exchange,and emotional behavior ability at different degrees.The improvement of confidence was very obvious.The speech communication ability of those with a hearing deprivation time longer than 5 years was lower than that of those with a shorter hearing deprivation time.Age and educational level did not influence the efficiency.Conclusion Cochlear implantation is a cost effective intervention in the posdingually deaf adults.NCIQ test can be used to evaluate the quality of life among postlingually deaf adult cochlear implant recipients in China.

Objective To investigate whether the PJ interval in the patients with pre-excitation syndrome can be shortened by pathway conduction,and to explore the clinical implications of the prolonged PJ interval.Methods 143 patients with single pathway,who experienced successful radiofrequency (RF) ablation,were divided into two groups:Group A (n=132) with normal atrioventricular and ventricular conduction (sub-divided into 10 subsets further according to the location of the pathway) and Group B (n=11) with first degreeatrioventricular block or with bundle branch block.The ECG images with and without pathway conduction were analyzed.Results (1) The PJ interval in the patients with right posterior pathway or with right septal pathway was shortened significantly after the RF[(226±18) ms vs (236±19) ms and (221±18) ms vs (238±31) ms respectively,both P<0.05];(2) The PJ interval in Group B was shortened to different extents.The PJ interval values in 4 patients with first degree atrioventricular block were shortened.but still beyond normal extent.The PJ interval values in 4 patients with bundle branch block were shorted to normal extent.Conclusion If the pre-excitation syndrome patients have normal atrioventricular conduction the PJ interval is normal or the PJ interval may be shortened.If the patients have prolonged atrioventricular conduction,the PJ interval may be shortened by the pathway prograde conduction.PJ interval prolongation indicates atrioventricular conduction delay or ventricular conduction block,but bundle branch block cannot be excluded when the PJ interval iS normal.

Objective To explore the brain activation mapping following odor presentation with event-related functional magnetic resonance imaging.Methods Ten healthy young volunteers with normal sense of smell and 5 anosmia patients,all right-handed,underwent routine otorhinolaryngological examination and T&T subjective smelling test.Odorant isoamyl acetate was delivered by olfactometer synchronously with inspiration birhinally for 10 times with the interstimulus interval of 60 seconds.Functional magnetic resonance imaging (fMRI) based on the blood-oxygen-level dependent effect was carried out on a 3.0 T scanner,using gradient-recalled echo and echo-planar imaging technique,and the image data were analyzed with SPM2 software.Results Functional activations were found in the volunteers with normal sense of smell,but not in the patients with anosmia.The activation regions were present in the orbitofrontal gyrus,anterior cingulated gyrus,piriform cortex,insular gyrus,amygdala,thalamus,basal nuclei,temporal gyrus,and frontal gyrus.There were more active regions in the right hemisphere than in the left hemisphere,and there were 314 and 57 active voxels in the right and left hemispheres respectively.Conclusion Olfactory event-related fMRI is an objective measurement of olfaction,and has potential clinical application value.

Objective To investigate the expression and significance of Glypican-3 in colorectal cancer.Methods Immunohistochemistry was used to detect the expression of Glypican-3 in 200 specimens of colorectal cancer and adjacent non-cancerous tissues resected during operation.Results Glypican-3 immunoreactivity was recognized in both the cytoplasm and cellular membrane.The Glypican-3 positive expression rate in the tumor samples Was 66.0% (132/200),significantly higher than that in the adjacent nontumor tissues(24%,48/200,P=0.019).The Glypican-3 expression rate was significantly correlated with the carcinoma invasion (P=0.023) and lymph node metastasis (P=0.015),but not associated with gender,age,tumor size,and difierentiation grade (all P>0.05).Conclusion Over-expression of Glypican-3 may play an important role in the genesis and development of colorectal cancer,and may be used as a new biological parameter in predicting invasion and metastasis of colorectal carcinoma.

Objective To explore the diagnosis,treatment,and prognostic factors of liver sarcoma.Methods The clinical data of 16 liver sarcoma patients,9 males and 7 females,aged 46.7(12-73),treated 1980-2005,were analyzed retrospectively.Results The most common symptoms were right upper quadrant pain (56.3%) and mass in the right upper abdomen (56.3%).Alpha fetoprotein (AFP) was negative in all the patients.None was correctly diagnosed by imaging examination preoperatively.Fifteen patients underwent surgical treatment,including radical operation in 7 patients (46.7%) and palliative resection/biopsy in 8 patients.1 patient took transcatheter hepatic artefial chemo-embolization rather than operation because of multifocal tumor.Pathological analysis diagnosed 6 patients (37.5%) as with leiomyosarcoma,5 patients (31.25%) with angiosarcoma,2 patients (12.5%) with fibrosarcoma,1 patient (12.5%) with epithelioid hemangioendothelioma,1 patient(12.5%) with myxoid liposarcoma,and 1 patient (12.5%) with undifierentiated embryonal sarcoma.The 1-year,3-year,and 5-year survival rates were 71.4%,41.7%,and 33.3% respectively.The 1-year,3-year,and 5-year survival rates of the patients who received R0 were 100.0%,83.3%,and 66.7% respectively,all significandy higher than those of the patients who did not receive R0 (all P=0.011).The 1-year,3-year,and 5-year survival rates of the patients with the tumor size<5 cm were 100%.100%.and 75%respectively,all higher than those of the patients with the tumor size>5 cm,however,not significantly (all P=0.084).Conclusion Liver sarcoma is difficult to be diagnosed preoperatively.Surgical resection is the mainstay of the treatment of liver sarcoma.R0 and tumor size are of prognostic values.

Objective To establish a predictive model for pancreatic cancer by using serum protein footprint map.Methods Surface-enhanced laser desorption/ionization (SELDI) ProteinChip technology was applied to screen abnormally expressed proteins in 92 pancreatic cancer patients,15 patients with benign pancreatic diseases,18 patients with other diseases of digestive tract,and 96 healthy volunteers.48 patients with pancreatic diseases and 48 healthy volunteers selected randomly constituted the training group.and 43 pancreatic cancer patients,48 healthy voltmteers,15 patients with pancreatic diseases,and 18 patients with malignant diseases of digestive tract selected randomly constituted the testing group.A predictive model was founded by bioinformation methods using the data of the training group.The data of the testing group was imported to this model to verify by blind method.The sensitivity and specificity of both diagnosis models and CA19-9,were analyzed to evaluate the clinical application value of the model in diagnosis of pancreatic cancer.Results Using IMAC3-Cu chip,12 peaks of differentially expressed proteins were discovered.Six of those had significant value in diagnosis of pancreatic cancer.With the principle of decision tree,a model was founded to diagnose pancreatic cancer,which was composed of 4 decisive nodes and 5 terminal nodes.With the blind test,the sensitivity of the model was 90.7%(39/43) and the specificity was 89.6%(43/48) in differentiating cancer from normal status.The modal was better than CA19-9.Serial tests could raise the specificity to 97.9%(47/48),while parallel test could raise the sensitivity to 95.3%(41/43).Conclusion The predictive model established by serum protein footprint map is a quick,easy,convenient and accurate method to diagnosis pancreatic cancer and screen new tumor markers.

Objective To investigate the expression of aquaporin (AQP)1 in retina during neovascularization.Methods Sixty C57BL/6J 7-day-old mice were randomly divided into 2 equal groups.The 30 mice of the model group were raised in 75%±2%oxygen for 5 days and then returned to condition with normal oxygen (room air) for 3-5 days.12,15,and 17 days after birth (on the postnatal day 12,15,and 17)10 mice from each group were kiUed respectively.Immunohistochemistry was used on 6 eyes and RT-PCR and Western blotting were used to detect the mRNA and protein expression of AQP1 in 7 eyes respectively.Thirty mice were raised in normal oxygen as controls.Results The retinal neovagcularization rate on P17 in the model group was 100%.AQP1 expression in retina was seen in 1 eye from the model and control groups each on P12,and in all mice of both groups on P15 and P17,especially in the lateral retina.AQP1 expression in retinal endothelial cells was seen in the model mice and not in the control mice.There were not significant differences in the AQPl mRNA expression level on P12 and P15 between the 2 groups,however.the AQPl mRNA expression level on P17 was significantly higher in the model group than in the control group(1.81±1.02 vs 1.15-4-0.01,P=0.000).AQP1 protein expression was seen on P12,P15,and P17 in both groups,and the AQP1 protein expression level at P17 of the model group was significantly higher than that of the control group(1.82±0.05 vs 1.57±0.04,p=0.000).Conclusion AQP1 protein may play an important role in retinal neovascularization.

Objective To investigated the effects of ischemic post-conditioning (IPO) on the lung endogenous oxidant-antioxidant system and nitric oxide level during the early stage of ischemia/reperfusion (I/R) injury.Methods Twenty four male Sprague-Dawley rats were randomly divided into 3 equal groups:sham-operation(S) group,I/R Group,undergoing ischemia by blocking the lung hilus for 1 h and then reperfusion for 30 min,IPO Group,undergoing blocking the lung hilus for 1 h and then 3 cycles of 10 s ischemia and 10 s reperfusion.Then the rats were killed with their lungs taken out.The levels of glutathione (GSH),nitric oxide(NO),malondialdehyde (MDA),xanthine oxidase(XO),and endogenous antioxidant enzymes,i.e,superoxidase dismutase (SOD),and catalase (CAT),and activities of guhathionine peroxidase (GPX) and myeloperoxidase (MPO),a neutrophil accumulation marker,were measured respectively.Results In IPO group,Compared with I/R group,antioxidant systems such as The levels of SOD,CAT,GPX,and GSH of IPO Group were (41.4 ±4.4) U/mg,(19.5 4±1.6)U/mg,(168±13) U/mg,and (1.72±0.26) U/g,all significantly higher than those of I/R Group[(19.6±2.8) U/mg,(8.4±0.8) U/mg,(72±8) U/mg,and (0.89±0.07) mg/g respectively,all P<0.05);and the levels of XO,MPO,and MDA of Group IPO were (50±6) U/g,(5.0±0.5) U/g,and (0.91±0.08) nmol/mg respectively,all significantly lower than those of I/R Group[(83±8) U/g,(7.6±0.7)U/g and (1.58±0.17) nmol/mg respectively,all P<0.05).The endogenous NO level of IPO Group was (93±7) umol/g,significantly hisher than that of I/R Group [(22±4) umol/g,P<0.01].Conclusion Post-conditioning at onset of reperfusion reduces lung I/R injury.The lung protection of IPO may be mediated,in part,by inhibiting the oxidant generation and oxidizing,and may be associated with the increasing of the endogenous NO level.

Objective To investigate the possible mechanism by which adiponectin influences the osteoclastogenesis.Methods Human osteoblasts were cultured in vitro and adiponectin of the concentrations 0,3,10,and 30 ug/ml were added for 48 hours to investigate the dose-dependent effect on the mRNA and protein expression of osteoprotegerin (OPG) in osteoblasts,and adiponectin of the concentrations 0 or 30 ug/ml was added for 0-48 hours to investigate the time-dependent effect.Fluorescent quantitation PCR and ELISA were used to detect the expression of OPG and receptor activator of nuclear factor-kB ligand (RANKL).Other human osteoblasts were cultured with CD14+peripheral blood mononuclear cells (PBMCs),and incubated together with adiponectin of the concentration of 30 ug/ml for 14 days,and then stained by antitartaric acid phosphatase,a marker enzyme of osteoclast.Results The expression levels of mRNA and protein of OPG in osteoblasts were 100%±9%,68%±7%,47%±8%,and 30%±5%,and (9.2±0.3),(6.6 4-0.4),(4.1±0.4),and (2.6 4-0.4) ug/ml respectively after incubation with adiponectin of the concentrations of 0,3.10,and 30 ug/ml for 48 hours.The mRNA and protein expression levels of RANKL were 100%±5%,165%±8%,213%±6%,and 316%±10%,and (1.3 4-0.2),(2.1 4-0.1),(2.3±0.2),and (2.8±0.1) ng/ml respectively.Adiponectin inhibited the osteoblast OPG expression and promoted the osteoblast RANKL expression dose-and time-dependently(P<0.05).Adiponectin induced the osteoclast formation in the coculture systems of osteoblast and PBMCs systems.Conclusion Adiponectin increases osteoclast formation via stimulating RANKL and inhibiting OPG production in the osteoblasts.which may be one of the mechanisms adiponectin influenees the osteoclastogenesis thereby.

Objective To investigate the effects of angiotensin receptor blocker (ARB) on triglyceride (TG) metabolism and mechanism thereof.Methods Zucker fatty (ZF) rats and Zucker lean (ZL) rats were fed with water containing 0.01% olmesartan medoxiomil,a hishly specific ARB for 4 weeks.Frequently sampled intravenous glucose tolerance test was conducted to calculate the area under the glucose (AUCG),insulin sensitivity index (SI),glucose effectiveness (SG),Blood glucose,TC,TG,nonesterified fatty acid (NEFA),and HDL-C were measured with standard assay kit.Triton WR-1339 technique was usod to detect the TG secretion rate (TGSR).After TGSR and FS-IVGTT the levers were collected.The total cholesterol (TC) of the liver was detected,and Sudan IV staining was used to detect the triglyceride in the liver.Enzymatic method was used to measure the TG and TC in the liver extract.Results The blood pressure of both groups was lowered in both strains to the same extent after olmesartan taking.The SI value of the ZF rats was (0.31±0.22)uU·ml-1·min-1·10-4 significantly lower than that of the ZL rats (3.54±0.30)uU·ml-1·min-1·10-4,P<0.01).Bergman's minimal model showed that the SG value of the ZF rats was (1.35±0.51)min-1·10-2,significantly lower than that of the ZL rats(3.40±0.14 min-1·10-2.P<0.01).The plasma glucose levels of the ZF rats was (11.4±2.6) mmol/L,significantly higher than hat of the L rats[(9.2±0.6) mmol/L,P<0.01].Olmesartan treatment substantially elevated both the baseline SI and SG levels of the ZF rats.The TG of the ZF rats was 6 times as high as that of the ZL rats.The plasma NEFA level after olmesartan treatment of the ZF rats was Olmesartan treatment.significantly lower than that before treatment[(2.70±0.69)mEq/L,P<0.01],however,the Dlasma TG level of the ZF rats after the treatment was not significantly different from that before treatment (P>0.05).The TGSR rate of the ZF rats was almost 6 times that of the ZL rats.Olmesartan treatment lowered the TG overproduction by half (0.56±0.08) mg·min-1·100 g-1 BW vs(0.30±0.07) mg·min-1·100 g-1BW,(P<0.01).The liver TG content of the ZF rats was 10 times that of tlle ZL rats.Olmesartan treatment lowered the liver TG content from (22.7±4.2) mg/g to(12.8±1.7) mg/g(P<O.01) without affecting the cholesterol content.Pathology showed that tlle fatty liver developed in the ZF rats was significantly ameliorated by olmesartan treatment.Olmesartan treatment had no significant effect onTG metabolism or insulin sensitivity in the ZL rats.Conclusion ARB improves the overproduction and accumulation of TG in the liver associated with insulin resistance,and does SO through mechanisms independent of its hypotensive action.

Objective To investigate the possible relationship between liver-specific insulin-like growth factor I (IGF-1) and the pathogenesis of breast cancer.Methods Fifly non-IGF-l deficient (LID) mice were randomly divided into 2 equal groups:Group I,fed with dimethyl-benzanthracene (DMBA) for 8 weeks to cause mammary cancer,and Group Ⅲ,fed with DMBA and ginsenoside Rg for 28 d;and 50 LID mice were randomly divided into 2 equal groups too Group II,fed with DMBA for 8 weeks,and Group Ⅳ,fed with DMBA and ginsenoside Rg for 28 d.The mice were killed after the cessation of DMBA use.The serum IGF-1 expression was detected with method Six Gene chips were used to detect the gene expression in the breast cancer tissues.Results The breast cancer rates were 66.67% in Group I and 33.33% in Group Ⅱ.36.00% in Group Ⅲ,and 12.00% in Group Ⅳ.The tumor size was (0.79±0.20) cm in Group I,(O.37±0.08) cm in Group Ⅲ,(0.32±0.08) cm in Group II,and (0.15±0.05) cm Group Ⅳ.The IGF-1 level of Group Ⅱ was (41.33±7.52) ng/ml,1/4 as high as that of Group I[(166.51±12.32)ng/ml],and the IGF-1 level of Group Ⅳ was(33.48±6.73)ng/ml,1/4 as high as that of Group Ⅲ[(155.84±11.34)ng/mi].Compared with those of the control mice,tlle breast cancers of the LID mice had longer latency.10wer incidence,and slower growth rate.The differential gene expression in different serum IGF-1 levels involred binding,metabolism,apoptosis,cell eyele,signal transduction,immune response,transcription regulation and interpretation regulation and so on.Among these genes,Coll1,Egln3,Glycam1,Irf6,Lgals7,Perp,Ragl,and Rbm35a genes were closely related to the incidence of breast cancer.Condusion IGF-1 plays a role as a risk factor in the onset and development of breast cancer by affecting the expression of many dliferentially expressed genes.

A 22-year-old male was diagnosed with acute non-lymphocytic leukemia (M2) due to dizziness in April 2006. After 2 courses of induction chemotherapy with DA (daunorubicin, cytarabine), the patient achieved complete remission. After that, consolidation therapy was given.

The patient was a 21-year-old male who complained of polydipsia and polydipsia for half a year.In February 2005, he had polydipsia without inducement and nocturia once/night.In April of the same year, he felt obvious polydipsia, and his daily water consumption reached 14 L, urine volume was 10~12 L, and nocturia was 3~4 times.

1. biofeedback principle biofeedback (BF) therapy refers to the application of modern science and technology to transform body biological signals, such as myoelectricity, brain electricity, skin temperature, heart rate and blood pressure, which people usually don't realize, into perceptible signals, such as vision, hearing or language, so that patients can increase the sensibility of biological organisms, restore or establish correct living habits according to these signals, and achieve effective treatment purposes [1]. 　　

A 40-year-old male was admitted to our hospital on January 15, 2007 after resection of retroperitoneal tumor for nearly 7 years. The patient was admitted to our hospital in February 2000 because of the discovery of a huge mass in the right upper abdomen. Preoperative enhanced magnetic resonance (MRI) of the upper abdomen (Figure 1) showed a huge lobular occupation in the right lobe of the liver, about 10.8 cm ×12.6 cm in size, showing uneven low signal in T1 weighting, high and low mixed signal in T2 weighting, and extremely high signal in some. The tumor enhancement was not obvious in the arterial phase of enhanced scan, and most of the tumors in the delayed phase showed uneven and obvious enhancement, so he was diagnosed as right liver cancer.

The patient was a 48-year-old female with a history of chronic gastritis for 3 years. In April 2003, she went to our hospital with epigastric pain aggravated for more than 3 months and accompanied by heartburn, acid reflux and vomiting for 1 week. There was no fever or weight loss. No positive physical examination was found. Gastroscopy showed that the gastric fundus mucosa was smooth, the gastric body and antrum cavity were narrowed, the mucosa was hypertrophic, the gastric angle was deformed, easy to bleed, the mucosa was uneven, and the surface was scattered with white coating (Figure 1). Endoscopic ultrasound showed that the submucosa was obviously thickened and clearly stratified, and the thickest part of the gastric wall was 9.2 mm (Figure 2). Early gastric mucosa-associated lymphoid tissue (MALT) lymphoma was diagnosed by large mucosal biopsy at the junction of gastric antrum and body (Figure 3). Rapid urease test (RUT) and urea breath test (UBT) showed Hp infection. Considering that this case was Ann Arbor stage IE, and the patient refused to undergo surgical treatment, the patient was given omeprazole 40 mg twice/d + amoxicillin 1.0 g twice/d + clarithromycin 500 nag twice/d after informed consent for 2 weeks.

The 7th China-Japan-Korea Helicobacter Conference was held in Kyoto, Japan from February 21 to 23, 2008. The conference was hosted by the Japanese side, with Professor Tsutomu Chiba as the chairman of the conference. The organizing committees of the three parties were Academician Fan Daiming, Chairman of the Gastroenterology Branch of the Chinese Medical Association, Professor Hyun Chae Jung of South Korea and Professor Toshio Fujiok of Japan. More than 180 representatives attended the conference. The Chinese representatives attended the conference included Professor Hu Pinjin, Vice Chairman of the Gastroenterology Branch of the Chinese Medical Association, Professor Hu Fulian, Head of the Helicobacter Pylori (Hp) Group of the Gastroenterology Branch of the Chinese Medical Association, Professor Liu Wenzhong and Professor Wang Jide, Vice Leaders of the Helicobacter Pylori (Hp) Group of the Chinese Medical Association. Experts from China, Japan and South Korea discussed six aspects of Hp, which are briefly reported below.

In recent 10 years, allograft of various tissue components has entered a brand new era, including knee joint, one hand, both hands, larynx, abdominal wall, and even penis transplantation has been successfully reported. In 2002, American doctors successfully "face transplantation" of mice, the first partial face allograft in the world was performed in France on November 27, 2005, and the first male face transplantation in China and the first in the world was performed in Xijing Hospital of the Fourth Military Medical University of China on April 13, 2006<A recent article in the New England Journal of Medicine reveals the condition of the first face transplant patient 18 months after surgery (N Engl J Med, 2007, 357: 2451-2460).

Your magazine published in 2003, volume 83, issue 12<A study on drug resistance surveillance of Gram-negative bacilli in hospital and community acquired infections in China. The author has different views on the drug resistance and susceptibility rates of Salmonella and Shigella in this article, which is pointed out here for discussion.