Volume 88  ·   Issue 12  ·  2008

Objective To assess the ability to detect myocardial bridging(MB)of dual-sourcecomputed tomography(DSCT)compared with conventional coronary angiography(CAG).Methods Both DSCT coronary angiography(DSCTCA)and CAG were performed on 53 patients diagnosed or suspected as with coronary artery disease(CAD),45 males and 8 females,aged 36-87.4D cine mode was performed on 16 of these 53 patients with MB shown on DSCTCA.and the CAG data were reviewed retrospectively by automatic cine method.The MB findings by both methods were compared.The diameters of segments proximal to and distal to MB,tunneled coronary arteries at systolic and diastolic phases were measured,and stenosis rate of tunneled coronary arteries was computed according to the equation.The relationship between stenosis rate of turineled artery and milking effect on 4D cine mode was analyzed.Results Of the 53patients who had undergone CAG;4 images of MB were detected in 3 patients based on the CAG findings,with a detection rate of 5.7%,significantly than that by DSCT(30.2%,21 images of MB in 16 patients,χ2=10.837,P=0.001).4D cine mode showed various degrees of"milking effect"and abnormal blood flow in 16 patients with MB.The stenosis rate of tunneled artery during systole was related to the"milking effect"rate on 4D cine mode of DSCTCA(r=0.640,P=0.006).Conclusion More vividly and reliably showing the"milking effect"than CAG,DSCT Can be used as a routine screening method to detect or exclode MB.

Objective To investigate the clinical and pathological features of benign ileocecal ulcerative lesions proven by ileo-colonoscopy.Methods The clinical data of 31 patients,17 males and 14 females,aged(47±17)(17-76)with distal ileal ulcers and/or ulcers of ileocecal valve,≥2 mm in diameter,confirmed by colonscopy were analyzed.Results The clinical manifestations included abdominal pain,lower right abdominal pain,diarrhea,constipation,hematochezia,fever,and body weight loss.Abdominal pain was found in 20 patients(64.5%)and lower right abdominal pain in 6(19.4%).20 patients had the ulcers 0.2-1.0 am in diameter and the ulcers of 11 patients were larger than 1.0 cm.The sites of ulcers included ileocecal valve(17 cases),anastomotic stoma after resection of ileoceeal valve(3 cases),distal ileum(7 cases),and both ileocecal valve and distal ileum(4 cases).Single ulcer was seen in 17 patients and multiple in 14.The ulcers were sharply demarcated.without remarkable inflammation around or between the ulcers.Histopathological findings were mostly unspecific,such as chronic inflammation,active inflammation,ulceration,eosinophil infiltration,inflammatory granulomation,granuloma,and thickness of vessel wall.Mostly,the causes of ulcer were of unknown origin(14 cases),especially for those of small size(12 cases).10 cases(32.3%)were diagnosed as Crohn's disease.The other diagnoses were intestinal tuberculosis,ischemia,Behcet's disease,AIDS,and NSAIDs-associated ulcers.Conclusion Most patients with benign ileocecal ulcers complain of abdominal pain,but lower right abdominal pain is not always present.The pathological findings are mostly not specific,but sometimes are critical for the diagnosis.The etiology was difficult to be confirmed and all the clinical data should be reviewed carefully.

Objective To summarize the experience in diagnosis and treatment of urachal mass in adults.Methods The clinical data of 33 Patients witll urachal mass.21 male and 12 females.aged 49(30-75),were analyzed retrospectively.Results The urachal masses of 11 patients(33%,11/33)were benign,including 5 cases of abscess,3 of cyst,2 of malacoplakia,and 1 of xanthogranuloma.The most common symptom of the benign urachal mass was abdominal mass(36%).Seven patients underwent computed tomography(CT)and no calcification was found.All 11 patients were treated successfully by mass excision.Twenty-two cases(67%)had urachal carcinomas,including 17 cases of adenocarcinoma,4 of squamous carcinoma.and 1 of transitional cell carcinoma.The most common symptom was gross hematuria(68%)in the malignant urachal mass patients and CT showed calcification in 38%of tllem(5/13).The overall 5-year cancer-specific survival rate Was 45.2%.Nine patients with localized cancer underwent extended partial cystectomy with a 5-year cancer-specific survival rate of 78.3%.The patients with metastatic urachal cancer underwent multimodal treatment with an objective response rate of 50%for chemotherapy and 25%for radiation tllerapy.The median survival time after metastasis of the 8 cases given multimodal treatment Was 14.0 months.7.5 montlls longer tllan that of the 4 cases untreated(6.5 months,P=0.09).Conclusion The majority of urachal mass in aduhs is malignant.CT helps in distinguishing the benignancy from malignancy of urachal mass.Surgical excision affords good course for benign urachal mass.Extended partial cystectomy provides a curative surgical treatment for localized urachal cancer.The prognosis of metastatic urachal cancer iS poor.Active muhimodal treatment may improve the survival of patients with metastatic disease.

Objective To analyze the characteristics of perioperative hemodynamics in pheochromoeytoma secreting different types of catecholamine,and to discuss how to improve the hemodynamics.Methods The clinical data of 202 patients with pheochromoeytoma who had received operation were analyzed.Three classification systems were used to divided the patients:they were,firstly,divided into high and low epinephrine secreting groups(Ehigh and Elo)according to the 24 hours urine catecholamine quantitation;secondly,into high and low norepinephrin secreting groups(NEhigh and NElow)according to 24-h urine norepinephrin quantitation;and thirdly,into high and low dopamine secreting groups(DAhigh and DAlow)according to the 24 hours urine dopamine quantitation.Relationship between the characteristics of perioperative hemodynamics and catechotamine secreting types were analyzed.Results The highest preoperative systolic blood pressure values of Ehigh and NEhigh groups were(201±40)mm Hg and(205±38)mm Hg,both significantly higher than those of the Elowand NElow groups[(183±43)mm Hg,P=0.003 and(181±43)mm Hg,P=0.000].The range of systolic blood pressure fluctuation during the operation of the Ehigh group was 108±39 mm Hg,significantly larger than that of the Elow group (91±33 mm Hg,P=0.001).The incidence rates of persisting postoperative hypotension of the NEhigh and DAhigh groups were 23.3%(20/86)and 32.7%(17/52)respectively,both higher than those of the NElow and DAlow groups[2.6%(3/116)and 4.O%(6/150)respectively,P=0.000 and P=0.000].Conclusion Different characteristics of perioperative hemodynamics are present in pheochromocytoma secreting different types of catecholamines.Patients with highly epinephrine secreting pheochromocytoma are apt to have larger blood fluctuation during the operation.Patients with highly norepinephrine and dopamine secreting pheochromocytoma are apt to suffer from persisting postoperative hypotension.

Objective To evaluate the clinical effect and restenosis rate of antiplatelet therapy following peripheral artery angioplasty and stenting.Methods After successful placement of peripheral artery stents to 103 patients with peripheral arterial occlusive disease(PAOD)in were randomized assigned to 2 groups:antiplatelet therapy group receiving clopidogrel 75 mg plus aspirin 100 mg(n=56)and control group(n=47)receiving anticoagnlation therapy low molecular weight heparin(LWMH)for 7 d plus longterm warfarin.The patients were f01lowed up 1 day,and 1,6,12,and 18 months after the operation to undergo color Doppler uhrasonography,and examinations of blood routine,bleeding time,coagulation time,and ankle-brachial Index.The primary endpoint events included major bleeding rate.and composite rate of restenosis and reocclusion.The secondary endpoint events included cardiovascular events,death,and adverse drug reaction.Results There were no significant differences in the baseline data between these two groups.The thrombotic occlusion rate was 1.8% in the antiplatelet group and 0% in control group.and the restenosis rate was 14.3% in the antiplatelet group and 25.5% in control group(both P＞0.05).The bleeding complication rate of the antiplatelet group was 1.8%.significantly lower than that of the anticoagulation group(19.1%,P＜0.01).There were not significant difierences in cardiovaseular event rate and mortality 18 months after operation between these two groups.Conclusion Antiplatelet therapy combined with clopidogrel plus aspirin is effective and safe in preventing restenosis following peripheral artery angioplasty and stenting.

：Objective To investigatethe influence of patella tracking and thickness for muscle strength after patellarresurfacing in total knee arthroplasty(TKA).Methods Quadriceps muscle strength of 100cases 134 knees with osteoarthritis were analysed with isokinetic testing and extensionlag after patellar resurfacing in TKA.Result was analyzed statistically through Ttest.Results Significant decreasing of quadriceps muscle strength and endurance weredetected on mal-pateUofemoral tracking(61±24,P＜0.01)and improper position ofpatella prosthesis(56±33,P＜0.01).Anterior knee pain with patella resurfacing wasmainly associated with malpatellofemoral tracking.Conclusion Asymptomatic weakness ofquadficeps muscle was mainly associated with impropcr patella thickness.

Objective To analyze the significance of pH monitoring and reflux symptom index(RSI)in the diagnosis of laryngopharyngeal reflux disease(LPRD)and the characteristics of LPRD in symptoms and pH monitoring.Methods RSI questionnaire survey and laryngopharyngeal pH monitoring were conducted on.31 patients with laryngopharyngeal reflux-related symptoms,15 males aged(50±13)years and 16 females aged(48±11)years,Gastroscopy was conducted on 13 patients to detect reflux esophagitis.The patients with the RSI scores≥13 were suspected as with LPRD and those with the reflux times≥7 or reflux area index(RAI)≥6.3 were diagnosed as with LPRD.McNemar test and Kappa test were performed on the RSI scores and reflux area indexes.Results Seventeen patients met the pH criteria.There were 8 patients suffering from voice disorders,17 from clearing throat,13 from excess throat mucus,11 from dysphagia,9 from cough after eating or with supine posture,9 from dyspnea,13 from cough,12 from foreign body sensation,and 14 from digestive symptoms with the foregoing symptoms'mean scores of 1.4,3.1,2.1,2.0,1.1,1.6,1.8,2.9,and 2.9 respectively.The P value with McNemar test on pH monitoring and RSI scoring was 0.289,and the P value with kappa test was 0.007.RSl was correlated with RAI(rs=0.302,P=0.049),reflux time(rs=0.454,P=0.005),and reflux time percentage(rs=0.439,P=0.007),but not correlated with the reflux frequency(rs=0.252,P=0.086).Supine reflux was seen in only 7 of the 224 reflux events in 17 patients,lasting only 96 seconds during the 84.6 minutes'supine acid exposure time.Conclusion The common symptoms of LPRD are clearing throat,digestive symptoms,excess throat mucus,and foreign body sensation.Laryngopharyngeal pH monitoring and RSI scoring have the same value in diagnosing LPRD.Acid exposure in laryngopharynx is correlation to patients'serf-report symptom.Almost all reflux events occur when the patients are in upright position.

Objective To investigate the differences in the expression of vascular endothelial growth factor(VEGF)and mcrovessel density(MVD)count in breast benign affection,breast atypical hyperplasia,and breast carcinoma in situ and to clarify the association of VEGF expression and MVD with the clinicopathological features of these diseases.Methods Immunohistochemistry(SP-method)was used to examiHe the expression of VEGF and MVD count in 100 samples of breast benign affection(including 35cases of breast fibroid tumor,35 cases of breast cystic hyperplasia,and 30 cases of intraductal papilloma),and 15 samples of breast atypical hyperplasia,and 25 samples of breast carcinoma in situ,obtained during operation.Results The positive rate of VEGF of the breast carcinoma in situ group was 56%,significantly higher than hose of the breast benign affection and breast atypical hyperplasia groups(22%and 33%respectively,P＜0.05).However,there was no significant differences in positive rate of VEGF among breast fibroid tumor,breast cystic hyperplasia,and intraductal papilloma(all P＞0.05).The MVD value of the breast carcinoma in situ group was 20.1±6.1.significantly higher than those of the breast benign affection group and breast atypical hyperplasia groups(14.3±3.5 and 18.5±3.6 respectively,both P＜0.05).There was no significant difierences in MVD value among breast fibroid tumor,breast cystic hyperplasia,and intraductal papilloma(all P＞0.05).Conclusion In breast tumors,angiogenesis is probably mediated mainly by VEGF.The occurrence and progression of breast cancer may be related with the expression of VEGF.

Objective To investigate the feasibility of ABC typing to redefine the subtypes of acuteonset type l diabetes mellitus(DM).Methods Radioligand assay was used to detect glutamic acid decarboxylase-antibody(GAD-Ab)and tyrosine phosphatase autoantibody(IA-2A)in 308 patients with acute-onset type 1 DM. The patients were thus divided into 2 groups:pancreatic islet auto-antibody ( demonstrated as A)positive group-positive in GAD-Ab or IA-2A-and pancreatic islet auto-antibody negative group.The clinical features and frequencies of HLA-DQ genotypes of these groups were compared.Within 24 hours after the correction of diabetic ketosis or diabetic ketoacidosis and after fasting for at least 8 h fasting and postprandial venous blood samples were collected to detect the fasting C peptide(FCP,demonstrated as B)reflecting the beta cell function,and postprandial C peptide(PCP).The patients were foilowed up for 2 years to know the insulin dosage(＜20 U/d or≥20 U/d).Receiver operating characteristic curve(ROC)was drawn to judge the values of fasting C peptide,body mass index(BMI,demonstrated as C)reflecting the central obesity,and HLA-DQ genotype in further typing among the A+and A-patients and the optimal cutoff points thereof.Results Compared with the A-group,the metabolic disorders at the onset were more severe,the insulin dosage at the 2-year follow-up was higher,and the percentage of susceptible HLA-DQ genotype was higher in the A+ group(P＜0.05 or＜0.01).The level of FCP(B)could be used to further subtyping in botll A+and A-groups,with the optimal cutoff point of 150 pmol/L in the A+group and with the optimal cutoff point of 250 pmol/L in the A-group.BMI could be used for further classification in only A-group with the optimal cutoff point of 24 kg/m2.HLA-DQ genotypes were of little value in further classification both in A+and A-groups.Conclusion ABC typing may be used as a new way to redefine the subtypes in acute-onset type 1 DM for prognosis.

In 2007, the surgical treatment of carotid arteriosclerosis and stenosis entered a new stage. On the one hand, the efficacy of carotid endarterectomy (CEA) was further affirmed, and the number of hospitals in China to carry out this operation gradually increased.

Objective To investigate the effects of contrast medium on the pancreatic pathology in severe acute pancreatitis(SAP).Methods Twenty-four Wister rats underwent injection of sodium taurocholate into the biliopancreatic duct to establish models of SAP and then randomly divided into 4 groups:Group A(SAP group),Group B(SAP models injected with normal saline injected group),Group C(SAP models with injeetion of 76%cardiografin),and Group D(SAP models injected with uhravist-300).Another 6 rats were used as blank controls.The rats were killed to undergo pathological examination of their pancreases.Modified Schmidt method was used to score the pathological changes.Results The modified Schmidt scores of Groups A,B,C,and D were(6.5 ±1.2),(7.7±1.0),(7.5±1.5),and (7.2±1.2)respectively,all significantly higher than that of the control group[(3.2±1.7),all P＜0.05].However,there was no significant difference among these 4 groups(all P＞0.05).Conclusion CM has no adverse effects on the pathology of pancreas with SAP and does not worsen the disease.

Objective To investigate the effect of gene Livin transfection on the apoptosis of human bladder transitional cell carcinoma(BTCC)cells.Methods Target fragment containing Livin full-length cDNA was obtained from the breast cancer cells of the line MCF7.Vector peDNA3.1(+)-Livin was constructed and transfected into the bladder transitional cell carcinoma(BTCC)cells of the line T24 [T24/Livin+cells].Another T24 cells were transfected with the eukaryotie expression vectors pcDNA3.1(+)[T24/pcDNA3.1(+)cells].T24 cells without transfection were used as blank control gruup.These T24 cells underwent G418 selection so as to screen the T24/Livin+cells stably expressing the target fragment.RT-PCR was used to detect the Livin expression level in the transfected cells.After the cells were treated with mitomycin C(MMC)for 24 h,MTT method was used to detect the inhibition rate.Acridine orange(AO)staining was used to observe the apoptotic cells.The apoptotic rate was observed by flow cytometry.Results RT-PCR results indicated that expression of Livin was positive in the 324/Livin+cells.while were negative in the T24/pcDNA3.1(+)and T24 cells.After being treated with MMC for 24 h,the apoptotic rate of the T24/Livin+ cell was(8.7±1.5)%.significanfly lower than those of the T24/pcDNA3.1(+)cells and T24 cells[(21.4±2.3)% and(19.6±2.3)% respectively,both P＜0.01 ].Conclusion The gene Livin increases the anti-apoptosis ability of tumor cells and the cell apoptosis induced by chemotherapy.

Objective To investigate the effects of liver X receptor(L)(R)on the expression of fatty acid synthase(FAS)in diabetic liver.Methods Sixteen-week-old male db/db mice with C57BL/6background were administered via garaging of TO901317(TO),a LXR synthetic agonist,at the dose of 3 mg·kg-1·d-1 or dimethyl sulfide(DMSO),a vehicle alone for 7 days.Then the mice were killed with their livers taken out to undergo immunohistochemistry to observe the distribution of FAS protein.Human hepatocellular liver carcinoma cell of the line HepG2 were cultured with TO(10 μmol/L)or DMSO for 24 hours.Another HepG2 cells were transfected with mouse FAS promoter-luciferase reporter recombinants with or without pcDNA3.1,LxR expression vector.or an active sterol regulatory element binding protein-1 c(SREBP-1 c)expression vector for 12 hours.Real-time PCR and Western blotting were used to detect thelevels of mRNA and protein of FAS and SREBP-1 c respectively.Luciferase reporter assay was utilized to examine the activity of mouse FAS promoter.Results FAS was abundantly expressed in the mouse livers,especially in the cytoplasm of liver cells.The FAS mRNA levels of the livers of the dh/db mice was about 5.5 times as high as that of the db/m mice(P＜0.01).The FAS protein levels in the livers of db/db anddb/m mice treated witll TO were 1.7 and 3.5 times higher than those of the control mice(both P＜0.05).The SREBP-1 mRNA levels in the liver ofthe db/m and db/db mice treated with TO were 2.4 and 2.1 times higher compared with the control mice(P＜0.05,P＜0.01).Luciferase test showed that the FAS promoter activity of the HepG2 cells treated with TO Was 1.5 times that of the control cells(P＜0.01).The FAS promoter activities of the HeDG2 cells transfected with LXR and SREBP-1 c were 1.9 and 1.6 times those of the control cells(botn P＜0.01).Conclusion LXRE directly or indirect(via SREBP-1 c)upregulates the expression of FAS gene in the diabetic liver.LXR may mediate the 1ipid accumulation in liver of diabetes.

Objective To evaluate the effects of cytokines on tlle proliferation and function of CD4+CD25+ regulatory T cell(Treg).Mellaotis Tregs were isolated from na(i)ve C57BL/6 mice spleen and lymph nodes.Mature dendritic cells(mDC)were isolated from DBA/2 mice,co-cultured with Tregs,and divided into 4 groups with or without interleukin-2(IL-2),interleukin-4(IL-4),and interleukin-15(IL-15)added into the culture fluid.Fluorescence.activated cell sorting(FACS)was used to detect the Treg proliferation and apoptosis with CFSE and annexin-V staining.The co-cuhure increased Tregs were divided into 5 groups:CFSE labeled naive CIM+ CD25-T cells,self-proliferated Treg,Treg mixedly cultured with IL-2 mDC,and Teff,Treg mixedly cultured with IL-4,mDC,and Teff,and Treg mixedly cultured with IL-15,mDC,and Teff,a control group included Teff co-cultured with mDC.FACS was used 5 d later to evalugte the suppressive function of the Treg Oil the Teff. The expression of Foxp3,indicating the phenotype of Treg was detected.Results FASC showed that the values of precursor frequency(PF)of the Tregs stimulated by IL-2,IL-4,and IL-15 were 31.3%,28.9%,and 34.5%respectively,all significantly higher than that of the control group(14.5% all P＜0.05),and the values of proliferation index(PI)of the Tregs stimulated by IL-2,IL-4,and IL-15 were 1.9,1.7,and 1.8 respectively,all significantly higher than that of the control group(1.5,all P＜0.05).,The apoptotic rates of the Tregs stimulated by IL-2,IL-4,and IL-15 were 12.8%,11.4%,and 12.7% respectively,all signifieanfly lower tllan that of the control group(28.9%,P＜0.05).The Foxp3 expression rate of the Tregs stimulated by IL-2,IL-4,and IL-15was 91.75%.Conclusion IL-2.IL-4.and IL-15 in the in vitro culture of Treg stimulate the Treg proliferation,reduce tlleir apoptosis,and maintain their suppressive function.The proliferated Tregs still maintain their phenotype,highly expressing Foxp3.

Objective To investigate the effects of high level hepatitis B virus(HBV)replication on the hepatocytes.QSG-7701 cells.Methotis Human hepatocytes of the line QSG-7701 were cultured and transfected with the plasmid pUC18-HBV1.2 or pUCl8 containing 1.2 full length HBV DNA by the standard calcium phosphate precipitation method.Other QSG-7701 cells were transfected with the plasmid pUC18 as controls.Cell growth curves were drawn for 7 days after transfection.Four 4 days after transfeetion,HBV DNA in the culture medium was detected by using fluorescence quantitative real-time PCR.Cell apoptosis was detected by using terminal deoxynucleotidyl transferase.mediated dUTP nick end labeling and electronic microscopy.Differential expressed genes were analyzed by using Oliga signal pathway micro-array.Results The curves of cell growth showed tha the amount of control QSG-7701 cells increased bv(8.3±1.2)times,significantly faster than the pUCl8.HBV1.2 transfected QSG-7701 cells that increased only by(1.1±0.2)times(P＜0.01).Four days after transfection,the HBsAg positive rate of the pUC18-HBV1.2 transfected cells was 35.4%±6.7%.and the apoptotic rate was 15.2%±4.3%.The HBV DNA level in the cuhure supernatant peaked 4 days adder transfection with the maximum value of(5.8±2.6)×106 copies/ml.Genes related to cell growth and apoptosis,such as CASP3(2.7981),CASP7(2.2643),3-Apr(3.5013),CDC2(0.4380),MAPK6(0.4447),and MAP3K2(0.2785),were differentially expressed.Conclusion Higll replicated HBV markedly inhibits the growth of hepatocytes and induces cell apoptosis.

Objective To investigate the effect of pioglitazone(Pio)on dendritic cell-(DC)specific intercellular adhesion molecule-3 grabbing nonintegrin(DC-SIGN)expression in DCs and explore the possible mechanism of Pio inhibiting DC adhesion and transmigration.Methods DCs derived from human peripheral blood mononuclear cells were cultured and divided into 6 groups:blank control group.Pio 0.1 μmol/L group,Pio 1.0 μmol/L group,Pio 10 μmol/L group,GW9662,a peroxisome proliferator activated receptor(PPAR)-γ antagonist,10 μmol/L group,and GW9662 10 μmol/L+Pio 10 μmol/Lgroup.Western blotting was used to detect the protein expression of DC-SIGN 24 h later.Hnman umbilical vein endothelial cells(HUVECs)were obtained and co-cuhured witll the DCs undergoing different treatmdnts.Immunofluorescence test was used to detect the protein expression of DC-SIGN.DCs labeled with 5-ehloromethylfluorescein diacetate(CMFDA)were added into the monocellular layer of fused Ecs.Blank DCs and DCs pretreated witll anti-DC-SIGN antibody were used as blank and experimental groups.Laser confocal microscopy was used to observe the adhesion ability of the DCs.HUVECs were inoculated into the upper chamber of Transwell plate and CMFDA-labeled DCs of above mentioned groups were added to the mono-cellular layer of these Ecs.Serum.free culture mediam witIl monocyte chemoattractant protein-1 was added into the lower chamber.Eight hours later the transmigration ability was observed.Results Westem blotting showed that the DC-SIGN protein expression levels of the DCs of tlle Pio 1.0 μmol/L and Pio 10 μmol/L group were 0.96 ±0.09 and 0.80±0.08 respectively.both significanfly lower than that of the blank control group(1.25±0.23,P＜0.05 and P＜0.01);and tlle DC-SIGN protein expression level of the GW9662 10 μmol/L+Pio 10 μmol/L group was 1.10 ±0.12.significantly higher than that of the Pio 10 μmol/L group(P＜0.05).Immunofluorescence test showed that the DC-SIGN protein expression levels of the DCs of the Pio 1.0 μmol/L and Pio 10 μmol/L group were 22.3 ±5.4 and 14.4±2.3 respectively,both signifieanfly lower than that of the control group(29.5±5.1,P＜0.05 and P＜0.01),and the DC-SIGN protein expression level of the GW9662 10 μmoL/L+Pio 10 μmol/L group was 2,4.9 ±4.3.significantly higher than that of the Pio 10 μmol/L group(P＜0.01),and not significantly different from that of the blank control group(P＞0.05).The adhesion rates of the Pio 1.0 μmol/L and Pio 10 μmol/L groups were 10.8%±2.0% and 7.6%±1.5% respectively.both significantly lower than that of the control group(13.4%±2.1%,P＜0.05 and P＜0.01);and the adhesion rate of the GW9662+Pio 10 μmol/L group was 12.1%±1.9%,significantly higher than that of the Pio 10 μmol/L group(P＜0.01),and not significantly different from that of the blank control group(P＞0.05).The transmigration inhibition rate of DCs of the Pio 0.1,1.0,and 10 μmol/L groups were 4.1%.12.9%,and 17.2% compared with the transmigration rate of the blank control group(P＜0.05,P＜0.05,and P＜0.01).The transmigration rate of the GW9662+Pio 10 μmol/L group was significantly higher than that of the Pi0 10 μmol/L group (P＜0.05),and not significantly different from that of the blank control group(P＞0.05).The transmigration rate of the anti-DC-SIGN intervention group was lower by 17.8%than that of the control group (P＜0.01).Conclusion Pio down-regulates the DC-SIGN protein expression and inhibits DC adhesion and transmigration tllreugh the pathway of PPAR-γ.

Objective To observe the effects of sulfur dioxide(SO2)on cardiac function of isolated perfused heart of rat and to explore the physiological regulation of endogenous SO2 on myocardial action.Methods The hearts of 64 Wistar rats were isolated,perfused with Krebs-Henseleit(KH)solution through Langendorff apparatus,and randomly divided into 8 equal groups:Four groups underwent perfusion of SO2 of the concentrations 1、10、100、1000 μmol/L respectively for 5 min and then perfused with KH solution for 15min.Eight hearts underwent perfusion of SO2 of the physiological concentration(10 μmol/L)for 20 min.The control group underwent perfusion of KH solution for 20 min.Eight hearts of the nicardipine group underwent perfusion of nicardipine,a L-type calcium channel blocker,2.5 μmol/L for 5 min,SO2 10μmol/L for 5 min,and then KH solution for 10 min.The heart in the hydroxamate(HDX)group underwent perfuslon of HDX,an inhibitor of SO2 endogenous generating enzymes,for 5 min,and then perfused by KH solution for 15 min.The heart rate(HR),difference of left ventricular pressure(LVP),left ventricular peak rate of contraction(+dp/dtmax),peak rate of relaxation(-dp/dtmax),and coronary flow(CF)were measured.Then transmission electron microscopy was conducted.Results SO2 concentrationdependently inhibited the left ventricular ±dp/dtmax,LVP,HR,and CF(all P＜0.01).The left ventricular±dp/dtmax,LVP,and HR were inhibited(P＜0.05)by the physiological concentration(10μmol/L)SO2 donor continuous perfusion for 20 min.During perfusion 20 min,the LVP,+dp/dtmax,-dp/dtmax,and HR after perfusion for 20 min of the physiological concentration(10 μmol/L)SO2 donor continuous perfusion group were(15±3)mm Hg,(485±74)mm Hg/s,(339±64)mm Hg/s,and(114±26)/min respectively,all significantly lower than those 5 min after perfusion[(23±7)mm Hg,(595±93)mm Hg/s,(436±83)mm Hg/s,and(159±31)/min,all P＜0.05].The LVP,+do/dtmax,-do/dtmax,and HR of the nicardipine group were(37±10)rfun Hg,(1025±287)mm Hg/s,(570±181)mm Hg/s,and(139±48)/min respectively,all not significantly different from those of the control group.The LVP,+do/dtmax,-dp/dtmax,and CF after perfusion of the HDX group were(50±11)mm Hg,(1167 ±270)mm Hg/s,(889±72)mm Hg/s,and(6.3 ±1.9)ml/min respectively,all significantly lower than those before perfuaion[(69 ±16)mm Hg,(1579 ±315)mm Hg/s,(1186±263)mm Hg/s,and(9.5±1.3)ml/min,all P＜0.05].Conclusion Exogenous SO2 has negative inotropic effect on myocardium.by the mechanism related to voltage-gated calcium channel.Nicardipine blocks the inhibitory effect of SO2 at physiological concentration.

There are 11 different subtypes of phosphodiesterase (PDE) distributed in mammals. Type 5 phosphodiesterase (PDE-5) in its protein family is the most important protein in regulating the level of cyclic guanylate (cGMP) in cells. It is expressed in lungs, platelets, various smooth muscles and some brain regions [1].

Objective To identify the proteomic differences between renaX cell carcinoma(RCC)and renal benign masses and to evaluate the diagnostic value of parallel and serial test combining with CT and surface enhanced laser desorption/ionization-time of flight-mass spectrometry(SELDI-TOF-MS).Methods Serum samples were collected from 96 patients with tenal tumors.62 RCC cases and 34 renal benign mass cases,all of which had been evaluated by CT before surgery.The sera were analyzed using IMAC-Cu2+ProteinChip system by SELDI-TOF-MS.The decision tree was generated by Biomark Pattern based on the sera of 42 RCC cases and 22 renal benign mass cases and was blind-tested by the rest sera samples.The parallel method and serial method combining CT and SELDI were also used to distinguish RCC and renal benign masses.Results The sensitivity and specificity of the decision tree were 85.7%(36/42)and 90.9%(20/22)respectively.The sensitivity and specificity of the double-blind test were 75.0%( 15/20)and 83.3%(10/12)respectively.CT showed higher sensitivity but lower specificity in detecting RCC.While combining CT with SELDI-TOF-MS,the sensitivity and specificity could be improved.Conclusion Three peaks with the moleeular weights of 4657.56,2955.95,and 3278.00 were detected which are potentially useful for differentiating RCC and renal benign masses.Using serial method combining CT and the decision tree based on these three proteins improves the sensitivity and positive predictive values of diagnosing RCC to 100%.

A 35-year-old male was admitted to the eugenics clinic of Liaoning Institute of Family Planning Sciences on June 7, 2007 because his wife had three adverse pregnancy and childbirth history. His wife was evacuated due to stillbirth more than 40 days after the first pregnancy, spontaneous abortion more than 2 months after the second pregnancy, and the third fetus died intrauterine on February 3, 2007, 12 days after the expected date.