Volume 87  ·   Issue 20  ·  2007

In recent 10 years, chemical biology, which uses target-specific active small molecule compounds as probes to study the vital activities of important cells, has developed rapidly, which has also greatly promoted the progress of molecular pathogenesis research and targeted therapy of diseases, especially malignant tumors [1-2]. Leukemia is a hematopoietic malignant tumor caused by acquired somatic mutation, and its incidence rate occupies the top 10 malignant tumors.

radiofrequency ablation (RFA) for hepatocellular carcinoma has attracted the attention of clinicians at home and abroad for its advantages of predictable control of thermal coagulation range, setting and feeding back the temperature of the treatment area, minimally invasive, small liver function damage, and significant curative effect on small hepatocellular carcinoma [1-4].

Skin aging is a complex biological process, mainly including endogenous aging and exogenous aging. Endogenous aging refers to the procedural natural aging process of skin with aging, while exogenous aging refers to the aging changes of skin caused by environmental factors, among which ultraviolet rays are the main influence, so it is defined as photoaging.

Objective to investigate the effect of routine oral quinolones on prevention of infection in patients with acute leukemia during neutropenia. Methods Among 309 patients with acute leukemia infection, 149 patients with routine oral quinolones could prevent bacterial infection during neutropenia (group A) and 160 patients without quinolones (group B). The epidemiological characteristics and susceptibility of bacterial flora and the clinical characteristics of infection in both groups were retrospectively analyzed, and the effect of quinolones on prevention was studied. Results There was severe neutropenia in both groups, and the absolute neutrophil value (ANC)<0.2×109/L lasted for 11 (0~43) days. The median number of days of fever was 8 (1~46) d. The prophylactic application of quinolones significantly reduced the normal bacterial flora (P =0.00), the proportion of E. coli increased significantly (P =0.00), and the distribution of other major pathogenic bacteria was not significantly different. The positive rate of E. coli extended spectrum beta lactamase (ESBL) in quinolone prevention and non-quinolone prevention areas was not significantly different (31.9%, 35.4%, respectively, P =0.61). The susceptibility of most antibiotics was not different between the two groups. The incidence of upper respiratory tract infection/tonsillitis (10.7%) decreased, and the relative proportion of digestive tract infection (14.4%) increased in the quinolone prevention group. The prophylactic application of quinolones could not reduce the incidence of sepsis (14.0%, 14.7%, respectively, P =0.86). The initial effective rates of initial empirical anti-infection treatment were 65.8%, 60.6% (P =0.35), and the final effective rates were 96.0%, 91.9% (P =0.14), respectively. The prophylactic application of quinolones had no negative effect on anti-infection treatment There was no significant change in the proportion of other bacterial flora. Conventional antibiotic prophylaxis did not increase the drug resistance of major pathogenic bacteria. Prophylactic oral quinolones could not reduce the occurrence of gastrointestinal infection and sepsis.

Objective to investigate the correlation between the polymorphisms of the multidrug resistance gene (MDR1) 12 exon C1236T, 21 exon G2677T/A, 26 exon C3435T gene and the prognosis of acute myeloid leukemia (AML) after chemotherapy. Methods 44 patients with AML were included in this study. The genotypes of MDR1 loci 12, 21 and 26 were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and direct sequencing. After the first course of induction chemotherapy, bone marrow morphological examination was performed to determine whether the patients were in complete remission by combining with clinical indexes. x2 was used to analyze whether the distribution of all alleles of MDR1 conforms to Hardy-Weinberg equilibrium, and the correlation between the rate of complete remission (CR) and clinical characteristics was analyzed by x2 analysis or Fisher's exact test. Results The frequencies of C1236T, G2677T/A and C3435T gene mutations in Chinese were statistically significant compared with those of other ethnic groups. The frequencies of the genotypes of the above three loci in Chinese healthy people were not different from those of acute myeloid leukemia There was no significant correlation between the polymorphisms of MDR1 C1236T and C3435T gene and CR rate (P =0.349, P =0.074); There was a significant correlation between MDR1 G2677T/A polymorphism and CR rate (x2=6.214, P =0.045), and the CR rate of patients with G/G genotype was lower than that of patients without G/G genotype (x2=6.142, P =0.013); Compared with those with non-C/T genotype, the CR rate was lower in patients with MDR1 C3435TC/T genotype (x2=3.991, P =0.046), and significantly lower than that in patients with T/T genotype (x2=5.134, P =0.023). Conclusion There are racial differences in the frequency of MDR1 exon 12, 21 and 26 genotypes, and there is no statistical difference in the frequency of the above three loci genotypes in AML patients compared with healthy people.

Objective to construct a leukemia cell adhesion-mediated drug resistance (CAM-DR) model from bone marrow stromal cells (BMSC) of patients with acute lymphocytic leukemia (ALL), and to explore the role of CAM-DR in leukemia cell drug resistance from a new angle. Methods CAM-DR model was constructed by co-culture of human lymphocytic leukemia Jurkat cell line and the adherent layer of ALL bone marrow stromal cells irradiated with 60Co ray, and the morphology and structure of the model were identified by SEM. The cytotoxic effect of daunorubicin (DNR) on Jurkat cells, the accumulation concentration and distribution of DNR in Jurkat cells were determined by MTT method and flow cytometry, respectively. Results The results of SEM showed that Jurkat cells adhered to the bone marrow stromal cell layer through pseudopod or migrated to the bone marrow stromal cell layer in CAM-DR model, and Jurkat cells were found to be nested in the "mesh" of bone marrow stromal cells. The results of flow cytometry showed that the model did not affect the accumulation of DNR and the distribution of drugs in Jurkat cells, but significantly inhibited the responsiveness of Jurkat cells to DNR. Conclusion The CAM-DR model based on leukemia BMSC was successfully constructed, which laid a theoretical and experimental foundation for further research on the drug resistance mechanism of leukemia cells.

Objective to observe the effects of gallocatechin gallate (EGCG), the main active ingredient in green tea extract, on photoaging and mutation of human skin fibroblasts (HSF) after multiple long and medium wave ultraviolet (UVA, UVB) irradiation.Methods Neonatal foreskin HSF was isolated and cultured, and divided into normal control group, EGCG intervention group, UVA group, UVA + EGCG group, UVB group and UVB + EGCG group; The dose of UVB irradiation was 30 mJ/cm2 and the dose of UVA irradiation was 10 J/cm2, and HSF irradiation was performed daily for 2 weeks. The concentration of EGCG was 25 μ g/ml in the pre-experiment. The expression of aging-related β-galactosidase in the cells was detected by histochemical staining method, and the aging of the cells was observed. The mutation frequency of hypoxanthine phosphoribosyltransferase (HPRT) gene was detected by cloning method, and the mutagenicity of ultraviolet irradiation and EGCG intervention were observed. Results (1) A small number of β-galactosidase positive cells were observed in normal control group and EGCG group.The ratio of positive cells in other groups was 43%±4% in UVB group; UVA group: 54%±4%; EGCG + UVB group: 64%±5%; (2) Spontaneous mutation rate of HPRT in normal control group and EGCG treatment group was very low. The mutation induced by UVB alone and UVA irradiation was 72 fold and 241 fold, respectively, while the mutation rate of HPRT in UVB + EGCG group and UVA + EGCG group was 52.78% and 32.37% lower than that in UVB alone and UVA group, respectively (t =2.0742 and 2.7042, both P<0.05). Conclusion UVA and UVB irradiation can up-regulate the aging probability of HSF, and EGCG can further up-regulate the aging probability. UVA and UVB irradiation can increase the HPRT gene mutation frequency of HSF, and EGCG can significantly reduce the HPRT gene mutation frequency.

Objective to compare the changes of photographs, histological examination and digital image analysis of skin texture before and after intense pulse light rejuvenation treatment, so as to provide reference for studying the mechanism of intense pulse light improving skin texture and texture and establishing clinical efficacy evaluation methods.Methods The photographs of 58 patients with intense pulse light rejuvenation before and after treatment were subjectively evaluated; 4 cases underwent pathological examination and immunohistochemical examination of collagen fibers, and quantitative analysis of pathological films with special staining of elastic and collagen fibers; Two cases were studied by transmission electron microscopy; The results showed that the effective rate of improvement of skin texture was 62.1%, the effective rate of improvement of pigmented lesions was 84.60%, and the effective rate of improvement of vascular lesions was 81.25%. Electron microscopy showed that the number of naive fibroblasts increased, the secretory activity of fibroblasts increased, and the collagen fibers were arranged neatly and densely woven into a network. Digital image analysis of skin texture showed that the depth and width of skin texture improved before and after treatment, and the differences in mean skin roughness, arithmetic mean skin roughness and mean skin smoothness were statistically significant (all P<0.01). The mechanism may be related to the enhancement of fibroblast activity, the proliferation and rearrangement of collagen fibers and elastic fibers.

Objective to evaluate the value of multi-slice spiral CT coronary angiography (MSCTA) in patients with coronary artery disease. Methods 64-slice MSCT coronary angiography was performed in 58 patients. Contrast agent was injected through the anterior elbow vein, calm inspiration and breath-holding scanning was performed. The images were transmitted to the workstation for volume reconstruction, multi-plane reconstruction, curved surface reconstruction and maximum density projection for image post-processing reconstruction. Selective coronary angiography was performed in 24 patients. The image quality of MSCT was analyzed and compared with selective coronary angiography. Results 64-slice MSCT coronary angiography clearly showed the main branches and branches of coronary arteries in 58 patients. Atherosclerotic plaques were found on 115 coronary arteries in 38 patients, and the type of plaque and the degree of stenosis were shown. The position of stent and vessel bridge and the distal blood flow were clearly shown in 5 cases after coronary artery stenting and 1 case after coronary artery bypass grafting. Conclusion 64-slice MSCT is an effective and reliable method for diagnosing coronary artery disease. It is a promising non-invasive examination method in the screening and diagnosis of coronary artery disease.

Objective to introduce an effective method to prevent biliary tract injury during laparoscopic cholecystectomy. Methods 629 cases of laparoscopic cholecystectomy were performed by this method from January 2003 to October 2005. Each patient underwent routine blunt anatomy by flushing and aspiration to expose the common hepatic duct before the cystic duct was severed, and then the cystic duct was severed after the common hepatic duct and the common bile duct were cleared. 7 cases were converted to open surgery, 5 cases were due to severe abdominal adhesions, 1 case was due to the formation of biliary intestinal fistula, and 1 case was converted to open surgery for radical gallbladder carcinoma due to intraoperative frozen reporting of gallbladder carcinoma. Conclusion the blunt dissection of common hepatic duct during laparoscopic cholecystectomy can effectively prevent intraoperative biliary tract injury, this method is simple, easy to learn and easy to popularize.

Objective to investigate the method of laparoscopic megaureteroplasty in the treatment of congenital obstructive megaureterosis in children and to evaluate the clinical efficacy of this surgery. Methods Three children with congenital obstructive megaureterosis (3 males, aged 7-24 months) underwent laparoscopic megaureteroplasty. The ureter was dilated freely by laparoscopy, cut off at the point of entering the bladder, and a double J tube was placed, connected with the end of the catheter with 7-0 silk wire, and the ureter and bladder were anastomosed by laparoscopy. Results The operation time was 80-200 min, the bleeding was 30-50 ml, and there were no complications. The double J tube was removed 10 days after operation, and there was no fever and low back pain. After 4 months to 2 years of follow-up, there was no obstruction or hydrops in the ureters in the three cases. Conclusion Laparoscopic megaureteroplasty has the characteristics of small injury, fast recovery and good effect in the treatment of children with megaureterosis. However, the long-term efficacy needs further follow-up observation.

Objective to establish a finite element model of craniofacial complex in children with constructed temporozygomatic suture, zygomatoxmaxillary suture, zygomatoxofrontal suture and pterygomandibular suture, analyze the response process of the zygomatic bone when impacted frontally, and explore the biomechanical characteristics of craniofacial trauma in children.Methods A 7-year-old female was selected to scan the craniofacial by GElightspeed 16-slice spiral CT, convert the raw data downloaded by CT workstation DICOM, and establish a three-dimensional finite element model of craniofacial complex. The suture was constructed by MSC and Patran, and the response process of the right zygomatic bone of the craniofacial complex was analyzed. Results When the impact point fell on the front of the zygomatic bone of the children's craniofacial complex, the stress in the impact area was concentrated, 46% of the impact load was conducted along the temporal process of the zygomatic bone to the temporal bone, and the stress was concentrated in the atlantooccipital joint area. 34% of the impact load was conducted to the maxilla through the zygomatoxofrontal suture, and 20% was conducted to the frontal bone through the zygomatoxofrontal suture. Conclusion When the children's craniofacial complex was impacted at the zygomatic bone, the stress was concentrated in the impact area and the atlantooccipital joint area, and the suture can show a large stress gradient, which is the site prone to injury, while the suture can weaken the stress conduction, Thereby alleviating deep tissue damage in the posterior part.

Objective to use magnified endoscopy combined with mucosal staining technique to find the flat pathological changes of large intestinal mucosal bulge and flat pathological changes. Methods 16 457 patients were examined by magnified colonoscopy to find the bulge and flat pathological changes of large intestine. The age and sex of patients were recorded, and the incidence, endoscopic manifestations and pathological types of bulge and flat adenomatous polyps of large intestine were analyzed. Results (1) A total of 2218 cases of adenoma and carcinoma were detected, and 170 cases (7.67%) of flat pathological changes were detected. (2) The average diameter of bulge and flat tumors was (29 ± 9) mm, and the average diameter of flat tumors was (17 ± 14) mm. (3) There were 1630 cases (79.59%) and 140 cases (82.35%) of bulging and flat tumors in the left colon, respectively. (4) There were 1051 cases (51.34%) and 95 cases (55.55%), 87 cases (4.25%) and 29 cases (19.96%), 180 cases (8.79%) and 30 cases (17.54%) of bulging and flat tumors, respectively. (5) There were 638 cases (31.15%) and 44 cases (25.88%), 337 cases (16.46%) and 34 cases (20.00%), 45 cases (2.20%) and 16 cases (9.41%) of mild dysplasia, moderate dysplasia, severe dysplasia and early carcinogenesis in bulging and flat tumors.

Objective to analyze and explore the rule of occurrence and development of chyloid leakage after radical gastrectomy and the methods of treatment and prevention. Methods 161 patients with gastric cancer underwent radical gastrectomy from D2 to D4 prospectively. During the operation, one double cannula was routinely placed near the celiac artery and the diaphragmatic angle of abdominal aorta for drainage. The chyloid in the drainage tube was closely observed after operation, and the microbial smear and culture of chyloid fluid were performed, and the infected chyloid fluid was treated with anti-infection. The occurrence and development rule of chyloid leakage were observed, and the timing of drainage tube removal was clarified. Results Among 161 patients undergoing radical gastrectomy from D2 to D4, 19 cases developed chyloid leakage, and the drainage volume was less than 250 ml/24 h in 8 cases, 250~500 ml/24 h in 7 cases, and 500~1500 ml/24 h in 4 cases. A large amount of Candida albicans was grown in the drainage fluid in 8 cases, and 5 cases were complicated with bacterial growth. The chyloid leakage was cured 10~90 days after operation. The drainage volume of the drainage tube of chyloid leakage was almost zero; The infected chyle fluid was milky white and sterile after treatment; Conclusion Chyloleakage can be cured if it is detected in time, drained adequately, treated with anti-infection and local continuous irrigation when fungal or bacterial infection occurs after gastric cancer D2~ D4 operation.

Objective to investigate the changes and significance of serum hepatocyte growth factor (HGF) levels in patients with unstable angina pectoris before, during and after percutaneous coronary intervention (PCI). Methods Seventy patients with unstable angina pectoris were divided into PCI group (49 cases) and non-PCI group (21 cases) according to whether or not they were treated with intervention. Both groups were treated with unfractionated heparin during PCI or coronary angiography, and the PCI group was treated with low molecular weight heparin for 7 days after operation. Serum HGF levels were measured by immune-linked immunoassay before, during, immediately after operation, 24 hours and 7 days after operation, respectively. Troponin I (cTnI) was measured in PCI group before and 24 hours after operation. Results The preoperative HGF levels in PCI group were significantly higher than those in non-PCI group (13 566 ng/L ± 3769 ng/L vs 1736 ng/L ± 604 ng/L, P<0.01). The immediate postoperative HGF levels in PCI group and non-PCI group were significantly higher than those before operation (11457 ng/L ± 2298 ng/L vs 967 ng/L ± 349 ng/L, P<0.01). The delayed decrease of serum HGF level in cTnI-positive group after PCI has a certain relationship with myocardial microinfarction.

Objective to investigate the distribution of HCV genotypes and the infection status of HCV type 1a in Yanbian area of northeastern China. Methods 44 HCV RNA positive samples from Yanbian area were subjected to HCV 5' non-coding region (5'NCR) complex enzyme analysis. Four samples of type 1a (Y2, Y4, Y6, Y8) were amplified and sequenced with 5'NCR and NS5B regions, and then compared with 27 HCV whole gene reference sequences (all from GenBank) to construct a genetic evolutionary tree. Results Among 44 samples, 19 (43.1%) of 1a/1b mixed type, 12 (27.3%) of 1b type, 8 (18.2%) of 2a/1b mixed type, 4 (9.1%) of 1a type, 1 (2.3%) of 2a type, 2b and 3-6 types were not detected. Among them, 4 samples of type 1a, Y2, Y4, Y6 and Y8, compared with the whole gene reference sequences of each genotype, the highest homology of 5'NCR and 1a reference sequence HC-J1 was 0.990, 0.990 and 0.990, respectively The highest homology of HC-J4 in NSSB region was 0.936, 0.957, 0.936, 0.936, respectively, and Tree analysis showed that it was Type 1b. Conclusion Type 1a/1b mixed infection was dominant in Yanbian region, followed by Type 1b and Type 1b/2a infection, which was significantly different from other regions of China.Analysis of 4 cases of Type 1a HCV in virus strain showed that the results of HCV 5'NCR and NS5B typing were inconsistent, which may be the manifestation of natural recombination of HCV genome during biological evolution.

Objective to investigate the status and influencing factors of social adaptability of children with Down syndrome. Methods From September 2004 to July 2006, 36 children with Down syndrome aged 52-167 months, 30 children aged 20-65 months and 40 children aged 43-144 months were tested by family environment questionnaire, picture vocabulary test and social life ability scale of infants-junior high school students. The scores of independent living ability, exercise ability, homework and self-management were all higher than those of children in the matched control group (20.0±4.8), (13.3±4.7), t =5.72, P =0.000; (7.5±1.4), (6.4±1.6), t =3.10, P =0.003; (8.2±2.4), (6.2±2.0), t =3.68, The ability to live independently was (20.0±4.8) vs (26.5±4.9) (t =5.84, P =0.000); Exercise capacity was (7.5±1.4) vs (11.4±3.3) points (t =6.76, P =0.000); Assignment was (8.2 ± 2.4) vs (14.4 ± 3.9) points (t =8.55, P =0.000); Communication was (8.3±3.6) vs (18.3±4.8) (t =10.38, P =0.000); Participation in group activities (9.6 ± 2.3) vs (17.1 ± 4.2) (t =9.76, P =0.000); Self-management was (5.9±2.6) vs (13.8±4.6) (t =9.25,P =0.000). ② Correlation analysis showed that the initial score of picture vocabulary test was significantly correlated with each dimension of life ability. Even after controlling the age factor, the correlation between each dimension and the initial score of picture vocabulary test was still statistically significant. ③ Multivariate stepwise regression analysis showed that the family structure of selected people of independent life ability entered the regression equation; Dimensional exercise ability has family structure and mother's education level into the regression equation; Conclusion the social life ability of children with Down syndrome lags behind that of children of the same age but is better than that of children of the same intellectual age. Family environment factors and neonatal medical history have different effects on them, which suggests that reasonable intervention methods can help to improve their social adaptability.

Objective to investigate the molecular mechanism of calorie restriction (CR) in the treatment of non-alcoholic fatty liver disease (NAFLD). Methods Twenty-five male Wistar rats were divided into two groups, one group was fed normal diet (NC group, 7 rats) and the other group was fed high fat diet (HFM group, 18 rats). After 2 months of feeding, the rats in HFM group were randomly divided into continuous high fat diet group (HF group, 9 rats) and 60% calorie restriction group (CR group, 9 rats). After 1 month, the rats were sacrificed. The changes of body weight, visceral fat content, fasting blood glucose, fasting insulin and blood lipid of the rats were observed. The ultrastructural changes of the liver of each group were observed under electron microscope. The mRNA expression of longevity gene SIRT1 was detected by RT-PCR, and the protein expression of SIRT1 was detected by Westerm blotting method. Results the rats in HF group had significant NAFLD. Compared with the normal control group, the visceral fat content (15.1 g ± 4.1 g vs 9.0 g ± 0.4 g), blood lipid (total cholesterol 2.61 mmol/L ± 0.29 Blood glucose (6.2 mmol/L ± 1.46 mmol/L vs 4.4 mmol/L ± 0.57 mmol/L), blood insulin level (29.22 mU/L ± 7.28 mU/L vs 13.09 mU/L ± 1.18 mU/L) The expression of SIRT1 in transcription and translation was significantly lower than that in the normal group (P<0.01). Compared with the HF group, the body weight, visceral fat content, blood lipid, blood glucose and blood insulin levels in the CR group were significantly reduced, the expression of SIRT1 was significantly increased (P<0.01), and the ultramicropathological changes of hepatocytes were also significantly improved. Conclusion CR has a significant reversal effect on NAFLD in rats, and the increase of SIRT1 expression in the liver caused by CR may be an important molecular mechanism to improve NAFLD.

Objective to investigate the regulatory effect of peroxisome proliferator-activated receptor γ coactivator 1 α (PGC-1 α) on apoptosis of human ovarian epithelial carcinoma cells. Methods Human ovarian carcinoma cell line HO8910 was cultured in vitro. In the experimental group, three control groups were added with adenoviral vector of green fluorescent protein (GFP) gene. In the Ad-GFP group (green fluorescence infection) and ad-PCG-1 α group (PCG-1 α green fluorescence infection), apoptosis was detected by Hoeches staining, and the apoptosis rate was measured by flow cytometry. The expression of apoptosis-related genes Bax and Bcl-2 mRNA was detected by quantitative polymerase chain reaction (RT-PCR). Normal ovarian cells of Chinese hamster (CHO) were selected as control, and apoptosis was detected by overexpression of PGC-1 α in the same method. Results Overexpression of PGC-1 α in human ovarian carcinoma cell line HO8910 could induce apoptosis significantly. The apoptosis rate was observed by light microscopy, and the apoptosis rate was 58.9% by flow cytometry Conclusion PGC-1 α can significantly induce apoptosis in human ovarian cancer cells, the upregulation of the expression of pro-apoptotic gene Bax and the decrease of the expression of anti-apoptotic gene Bcl-2 may be one of the mechanisms of inducing apoptosis. PGC-1 α has a specific role in promoting apoptosis of tumor cells.

Iodine deficiency can cause irreversible brain injury in fetus and infant, as well as psychomotor development disorders in children.Iodine deficiency is the most common cause of preventable intellectual disabilities.It also affects reproductive function and hinders children's hearing.In 1920, the method of iodizing salt began to be used abroad to prevent and treat iodine deficiency. Over the years, this method has been proved to be the best, effective and safe method. Since 1994, China began to implement the Regulations on the Administration of Iodizing Salt to Eliminate the Harm of Iodine Deficiency. In the past 10 years, the work of eliminating iodine deficiency in China has been on the right track. Last year, the Chinese government solemnly declared to the world that China has basically achieved the stage goal of eliminating iodine deficiency diseases.

A case of acute leukemia with special gene was found in the diagnosis and treatment of leukemia in Beijing Daopei Hospital. The patient was a 34-year-old man who was admitted to the hospital in February 2006 due to fever for 10 days. At the onset of the disease, the blood routine: leukocytes 254×109/L, hemoglobin 86 g/L and platelets 59×109/L. The morphology of bone marrow cells considered acute myeloid leukemia (AML-M2). Immunotyping showed that there were two groups of myeloid and B lymphocyte blast cells in bone marrow, and the chromosome had special t (9; 22) and t (7; 14) translocation.

asparaginase is one of the main chemotherapeutic drugs in the treatment of acute lymphocytic leukemia (ALL), and plays an important role in the induction and post-induction of ALL. Clinical reports have confirmed that the disease-free survival and overall survival of ALL patients treated with high dose of asparaginase are significantly better than those who do not receive this drug.However, the drug resistance problem limits the drug to play a better efficacy in ALL, and the mechanism is still unclear.

As a special type of chronic pancreatitis (CP), autoimmune pancreatitis (AIP), which is mediated by autoimmune mechanism and characterized by pancreatic enlargement and irregular pancreatic duct stenosis, is attracting more and more attention [1-4]. AIP is effective in steroid therapy without surgical intervention, but in the past, it was often misdiagnosed as pancreatic cancer and surgically resected. AIP accounts for 2.2% ~2.4% of all pancreatectomies and 19.5% ~28% of benign pancreatic disease surgery [2].