Volume 86  ·   Issue 04  ·  2006

Interventional radiology is one of the important advanced technologies in radiology. It includes two categories: percutaneous non-transvascular technique and percutaneous transvascular technique. CT-guided interventional radiology belongs to the former, which includes CT-guided percutaneous biopsy and interventional therapy. It is a minimally invasive technique and is getting more and more attention from clinicians. CT can be used to guide various interventional techniques in the whole body, and all parts that cannot be guided by fluoroscopy and ultrasound can be guided by CT. CT scan has high resolution and good contrast, and can clearly show the size, shape and location of lesions, as well as the spatial relationship between lesions and adjacent structures. CT-enhanced scan can further understand the blood supply of lesions and the relationship between lesions and blood vessels. CT-guided technique can accurately determine the needle insertion point, angle and depth, avoid damaging blood vessels, nerves and important structures adjacent to lesions, and improve the accuracy and safety factor of interventional technique.

Objective to investigate the value of CT value of non-enhanced spiral CT in predicting the emptying rate of upper urinary tract calculi after extracorporeal shock wave lithotripsy (ESWL). Methods 52 patients with single upper urinary tract calculi with calculus size ranging from 0.5 cm to 1.5 cm and requiring ESWL were examined by non-enhanced spiral CT, and the CT value of the calculi was measured. After ESWL, B-mode ultrasound and kidney, ureter and bladder examination were followed up for 3 months. According to whether the lithotripsy remained or not, 52 patients were divided into stone emptying group and stone remaining group<The basic characteristics, CT values of stones and the number of energy shocks were statistically analyzed between the two groups. Results 36 cases (69.2%) had stone emptying and 16 cases (30.8%) had stone residual. The average CT values of the total stone emptying group and the residual group were 580 HU±195 HU and 1032 HU±271 HU, respectively (t =6.842, P<0.01), the difference was statistically significant; The average number of energy shocks in the total stone emptying group and the stone residual group were 1527 ± 723 and 2613 ± 519 respectively (t =5.407, P<The CT value and the OR value of the number of energy shocks were 0.993 (95% CI: 0.988-0.998) and 0.998 (95% CI: 0.997-1.000), respectively. Conclusion The CT value of non-enhanced spiral CT can predict the crushing rate and emptying rate of upper urinary tract stones after ESWL. The stones with low CT value are easily crushed and emptying after ESWL; The rate of crushing and emptying of stones decreased significantly in patients with higher CT values, and ureteroscopic lithotripsy and percutaneous nephrolithotripsy could be considered for these patients.

Objective to investigate the changes and trends of clinical epidemiological characteristics of colorectal carcinoma in Guangdong in recent 20 years. Methods the data of 3870 cases of colorectal cancer diagnosed by pathology in Guangzhou Nanfang Hospital and Huizhou Central People's Hospital from 1985 to 2004 were summarized and analyzed. The data of 20 years were analyzed every 5 years. Results Compared with the period from 1985 to 1989, the admission rate of colorectal cancer increased by 102.0%, with an average annual increase of 5.1%; The median age of onset increased from 50.2 years to 58.6 years, an increase of 8.4 years; The proportion of male and female cancer decreased gradually from 1.50 to 1.35, the proportion of rectal cancer decreased from 64.8% to 49.7%, and the proportion of right colon cancer increased from 18.0% to 28.7%; In histological types, the proportion of moderate and highly differentiated patients increased from 70.1% to 80.6%, while the proportion of poorly undifferentiated patients decreased from 29.9% to 19.4%; The proportion of colorectal cancer diagnosed with Dukes stage A increased from 3.2% to 9.8%. Conclusion the incidence of colorectal cancer in Guangdong has increased in recent 20 years; The median age of onset increased; The male-female ratio declined; The proportion of rectal cancer decreased, and the proportion of right colon cancer increased; The proportion of histological types with high school differentiation increased, while the proportion of low undifferentiation decreased; The proportion of diagnosed Dukes stage A increased.

Objective to investigate the relationship between the expression of cyclooxygenase (COX) -2 and the proliferation and apoptosis of tumor cells, and to explore the use of RNA interference technique as a method of tumor gene therapy. Methods the eukaryotic expression plasmid WBH1 targeting short hairpin double-stranded RNA (shRNA) of COX-2 was constructed and transfected into human gastric cancer cell line SGC-7901. The inhibitory effect was detected by reverse transcription polymerase chain reaction (RT-PCR) and Western blot from the mRNA and protein levels, respectively. The proliferation and apoptosis of the cells after the expression of COX-2 was inhibited were detected by tetramethylazolazole blue (MTT) and flow cytometry. The normal control group was inoculated with untransfected gastric cancer cells; In the negative control group, gastric carcinoma cells transfected with negative control plasmid were inhibited. After 4 weeks, the gross and pathological conditions of subcutaneous tumor formation in nude mice in each group were observed and compared, and the tumor inhibition rate was calculated. Results WBH1 effectively and specifically inhibited the expression of human COX-2, and the inhibition rate reached 70.42%. After the expression of COX-2 was inhibited, the cell proliferation was significantly inhibited (P =0.002), and the apoptosis rate was significantly increased. The apoptosis rate was statistically significant compared with that of gastric carcinoma cells not transfected and transfected with negative control plasmid (52.28%±17.91%, 0.52%±0.27%, 0.54%±0.16%, P =0.009, the experiment was repeated 5 times). All nude mice in the normal control group and the negative control group had tumor formation, and the average tumor weight was (0.490 g±0.017 g,5 mice) and (0.490 g±0.013 g,5 mice), respectively. Only 2 mice in the inhibition group had tumor formation, and the average tumor weight was 0.050 g±0.003 g, which was statistically significant compared with the normal control group (P<Conclusion COX-2 is closely related to the proliferation and apoptosis of tumor cells, and inhibiting the expression of COX-2 in cells can inhibit the proliferation and promote the apoptosis of tumor cells. The expression of COX-2 in human gastric cancer cells can be inhibited efficiently and specifically by constructing eukaryotic expression vector targeting COX-2.

Objective to construct a recombinant eukaryotic expression plasmid of parathyroid hormone (PTH) gene, evaluate the expression and biological activity of PTH gene after transfection in vitro, and observe its gene therapy effect on hypoparathyroidism animals.Methods (1) PTH gene was cloned from human embryonic parathyroid tissue, and its recombinant eukaryotic expression plasmid pcDNA3.1-PTH-GFP (pcDPG) was constructed by topological method, and identified by enzyme digestion, polymerase chain reaction (PCR) and DNA sequencing; (2) pcDPG was transfected into 293 cells by liposome, and the expression of green fluorescent protein (GFP) was observed and the transfection rate was calculated. At the same time, the expression of PTH gene was verified by reverse transcription-polymerase chain reaction (RT-PCR); (3) Purifying PTH protein in the supernatant of transfected cells and identifying the biological activity; (4) A rabbit model of hypoparathyroidism was established. pcDPG plasmid was injected intramuscularly for group therapy. The blood calcium, phosphorus and PTH values and survival time were monitored, and the pathological changes of each organ were observed by morphology. Results (1) The results of enzyme digestion and PCR were the same as expected, and the sequence homology of the sequence sequence was 99.3% with that in the literature; (2) The expression of GFP was observed at 24 h after transfection, and the expression increased with time. The transfection rate reached 38.9% and 62.5% at 48 h, and the expression of PTH gene was observed by RT-PCR; (3) the purified PTH protein can combat the convulsive symptoms of parathyroidectomized mice; (4) The serum calcium (1.71mmol/L±0.09 mmol/L,1.73 mmol/L±0.03 mmol/L,1.75 mmol/L±0.03 mmol/L,1.65 mmol/L±0.04 mmol/L) was significantly lower than that before operation (2.82 mmol/L±0.21mmol/L,P<0.05), PTH values on the second day after surgery (5.03 pg/ml ± 0.05 pg/ml, 5.04 pg/ml ± 0.05 pg/ml, 5.03 pg/ml ± 0.07 pg/ml, 5.29 pg/ml ± 0.03 pg/ml) It was also significantly lower than that before operation (11.63 pg/ml ± 1.60 pg/ml), but blood phosphorus increased on the second day after operation (P<Conclusion Liposome-mediated plasmid pcDPG has a higher transfection rate in vitro, can express active PTH protein, and has a better curative effect on hypoparathyroidism rabbits, thus laying a foundation for further research on gene therapy of hypoparathyroidism.

Objective to study the effect of Helicobacter pylori (Hp) infection on the expression of gastric and spinal cord neurons in mice, and to explore the possible mechanism of dyspepsia induced by Hp infection. Methods A mouse model of Hp infection was established. 35 female C57BL/6 mice were divided into 3 groups: acute infection group (15 mice 2 weeks after infection) and chronic infection group (15 mice 12 weeks after infection), and mice gavaged with normal saline were used as controls (5 mice control group). The histological changes and Hp colonization density of gastric mucosa of mice were evaluated by HE staining and Warthin-Starry staining, and the positive neurons of Fos, vasoactive intestinal peptide (VIP) and calcitonin gene-related peptide (CGRP) in gastric and spinal cord were evaluated by immunohistochemical staining. Results Three mice died at 12 weeks after infection with Hp, and the colonization density of Hp was significantly higher than that at 2 weeks after infection, and it was mainly distributed in pyloric gland mucosa. The positive degrees of Fos, VIP and CGRP neurons in stomach and spinal cord of mice were not statistically significant (all P>0.05) in the group 2 weeks after Hp infection (15 rats, stomach: 3.1±1.4, 4.5±1.8 and 2.4±0.8, spinal cord: 3.8±1.2, 3.2±1.5 and 2.2±0.6, respectively) and the group 12 weeks after infection (12 rats, stomach: 3.1±1.3, 3.5±1.6 and 2.2±0.8, spinal cord: 3.4±0.7, 2.6±1.2 and 2.5±1.1, respectively), but they were significantly higher than those in the control group (5 rats, stomach: 2.4±0.9, 1.6±0.9 and 1.2±0.8, spinal cord: 2.0±1.6, 1.2±1.1 and 1.2±1.1, respectively)<Conclusion Acute and chronic Hp infection induced different degrees of inflammation and activity of gastric mucosa in mice, and significantly increased the expression of Fos, VIP and CGRP positive neurons in stomach and spinal cord, which may be the basis of symptoms in patients with Hp positive dyspepsia.

Objective to investigate the regulatory effects of gastrin receptor antagonist proglutamine and specific cyclooxygenase-2 (COX-2) inhibitor NS-398 on the proliferation and apoptosis of human gastric adenocarcinoma cell line MKN-45. Methods MKN-45 cells were routinely cultured in RPMI-1640 culture medium. After the cells grew to submonolayer, proglutamine (final concentration 5.0 mmol/L) and/or NS-398 (10.0 μ mol/L) were added to the cells, and the cells were continuously cultured for 48 h. The cell proliferation was examined by tetramethylazolazole blue (MTT) colorimetric analysis, apoptosis was detected by flow cytometry, and the mRNA and protein expression of apoptosis inhibitor gene bcl-2 were detected by reverse transcription polymerase chain reaction (RT-PCR) and Western blot. Results Proglutamine and NS-398 synergistically inhibited the proliferation of MKN-45 cells. The apoptosis rates of proglutamide group, NS-398 group and combination group were 24.7% ± 3.2%, 26.7% ± 3.4% and 36.1% ± 4.6%, respectively, which were significantly higher than those of control group (1.6% ± 0.6%)<0.01); The apoptosis rate in the combination group was significantly higher than that in the single drug group (P<0.05). Proglutamine and NS-398 significantly downregulated the expression of bcl-2 mRNA and protein (both P<Conclusion Proglutamine and NS-398 inhibit the proliferation of MKN-45 cells and induce apoptosis by down-regulating the expression of bcl-2 gene. The combination of proglutamine and NS-398 has a synergistic anti-cancer effect.

Objective to investigate the role of caudal homeobox transcription factor 2 (Cdx2) in the development of intestinal gastric carcinoma. Methods the expression of Cdx2 in 104 cases of chronic superficial gastritis, 98 cases of chronic atrophic gastritis, 112 cases of gastric intestinal metaplasia, 106 cases of gastric dysplasia and 93 cases of intestinal gastric carcinoma was detected by immunohistochemical ABC method. Results Cdx2 was not expressed in chronic superficial gastritis and chronic atrophic gastritis, but the positive expression rates in gastric metaplasia, gastric dysplasia and intestinal gastric carcinoma were 86.6% (97/112), 58.5% (62/106) and 56.9% (53/93), respectively.<0.05); There was no significant difference in the positive rate of Cdx2 protein expression between gastric mucosal dysplasia and intestinal gastric cancer (P>0.05). Conclusion Cdx2 may be a specific marker of intestinal metaplasia in gastric mucosal tissues; Overexpression of Cdx2 protein plays an important role in the development of intestinal gastric cancer.

Objective to investigate the interaction between oxidized low density lipoprotein (ox-LDL) and caudopressin Ⅱ (UⅡ) on the proliferation of rat vascular smooth muscle cells (VSMC) and the effect of ox-LDL on the expression of UⅡ receptor. Methods The effects of different concentrations of ox-LDL (0.01, 0.1, 1, 10, 50 μ g/ml) and UⅡ (10, 50 nmol/L) on the proliferation of rat VSMC were determined by MTT assay. Competitive RT-PCR and radioligand receptor binding assay were used to analyze the changes of GPR14 expression in VSMC incubated with different concentrations of ox-LDL from the mRNA and receptor functional levels, respectively. Results ox-LDL 0.01 μ g/ml and UⅡ 10 nmol/L could synergistically promote the proliferation of VSMC, and the MTT value increased to 162%±29% (0.528±0.036 vs 0.308±0.026, P<0.01); Whereas at 0.1 μ g/ml the MTT value decreased to 153% ± 22% of that of the control (0.461 ± 0.017 vs 0.308 ± 0.026, P<0.05); At 1 μ g/ml, the synergistic effect of ox-LDL disappeared, and the MTT value decreased to 123%±13% (0.400±0.015 vs 0.308±0.026, P>0.05). At 50 μ g/ml, ox-LDL showed cytotoxicity and inhibited cell proliferation, and the MTT value decreased to 59% (0.195±0.017 vs 0.308±0.026, P<Competitive PCR showed that ox-LDL 0.1 μ g/ml could increase the expression of GPR14 mRNA by 25% (P<The concentration-dependent down-regulation of GPR14 mRNA was observed at 1~50 μ g/ml. The maximum effect was 73% at 50 μ g/ml (P<Incubation with ox-LDL 0.1 μ g/ml for 12 h increased the maximum binding capacity (Bmax) of 125I-UⅡ to VSMC by 18% (P<0.01), while ox-LDL 50 μ g/ml reduced Bmax by 69% (P<Conclusion UⅡ and ox-LDL can synergistically promote the proliferation of VSMC in a certain concentration range; Low concentration of ox-LDL can up-regulate the expression of GPR14 in VSMC, while high concentration can down-regulate this receptor.

Objective to investigate the effects of inhibition of nuclear factor-κ B (NF-κ B) activation on multiple organ injury in the rat model of ruptured abdominal aortic aneurysm (RAAA). Methods 45 Wistar rats were randomly divided into sham operation group, experimental group and pyrrolidine dithiocarbamate (PDTC) group (15 rats in each group). The pathophysiological process of ruptured abdominal aortic aneurysm in the experimental group and PDTC group was simulated by rat models: 1 h in hemorrhagic shock, 45 min in abdominal aortic occlusion and 2 h in reperfusion. The experimental group and PDTC group were given normal saline or PDTC 50 mg/kg intravenously during hemorrhagic shock. The tissues of lung and small intestine were collected, and the histomorphological changes were observed. The wet/dry weight ratio of lung tissue, myeloperoxidase (MPO) and the changes of small intestinal microcirculation blood flow were measured. The mRNA expressions of NF-κ B p65 and intercellular adhesion molecule (ICAM) -1 in lung and small intestine were detected by reverse transcription polymerase chain reaction, and the protein expressions of NF-κ B p65 and ICAM-1 were detected by immunohistochemistry and Western blot.<0.01); The small intestinal microcirculation blood flow was significantly reduced compared with that of sham operation group (0.71 ml·min-1·g-1±0.11 ml·min-1·g-1) (P<0.01); The expression of mRNA and protein products of NF-κ B p65 and ICAM-1 in lung and small intestine of the experimental group were significantly enhanced; After PDTC intervention, the small intestinal microcirculation blood flow (0.64 ml·min-1·g-1±0.06 ml·min-1·g-1) was significantly different from that of the experimental group (P<Conclusion Inhibition of NF-κ B activation can alleviate the functional impairment of multiple organs in RAAA model in rats, and its mechanism may be achieved by inhibiting the expression of ICAM-1 gene and its protein products and reducing the effect of neutrophils.

Objective to investigate the mechanism of valsartan in reversing myocardial interstitial fibrosis in diabetic cardiomyopathy (DCM). Methods 40 male Wistar rats were randomly divided into 3 groups: control group (8 rats), DCM group (16 rats) and valsartan intervention group (16 rats). Insulin resistance was induced by high-fat and high-calorie diet, and DCM animal model was established by injection of low-dose streptozotocin (STZ). The changes of collagen content, TSP-1, TGF β 1 mRNA and protein expression in left ventricular myocardium were detected by Masson staining, real-time quantitative reverse transcription-polymerase chain reaction and Western blot technique. Results Compared with the control group, the collagen content in left ventricular myocardium of DCM rats was significantly increased (11%±3% vs 17%±3%, P<0.01), presence of myocardial interstitial fibrosis; The expression levels of TSP-1 mRNA and protein were significantly increased (0.0089 ± 0.0034 vs 0.0141 ± 0.0037, 96 ± 17 vs 130 ± 17, all P<0.05), TGF β 1 mRNA, active and inactive TGF β 1 protein expression levels were also significantly increased (0.0054 ± 0.0009 vs 0.0126 ± 0.0057, 103 ± 18 vs 143 ± 17, 99 ± 20 vs 155 ± 35, all P<0.01); Conclusion Valsartan plays an important role in reversing the development of myocardial interstitial fibrosis in DCM by inhibiting the TSP-1/TGF β 1 signaling pathway.

Objective to evaluate the clinical value of CT-guided 125I seed implantation in the treatment of pancreatic carcinoma. Methods the clinical data of 26 patients with pancreatic carcinoma treated by CT-guided 125I seed implantation from June 2003 to May 2004 were retrospectively analyzed, and the experience and technique of this operation were summarized. Among the 26 patients, 21 were males and 5 were females, aged 47-73 years. The median age was 60 years ± 13 years. The average diameter of the lesions was 6.1 (1.0-8.5) cm. Among them, 15 cases had severe abdominal pain and 10 cases had jaundice. All cases were clinically diagnosed as pancreatic cancer after CT, MRI (14 cases) or pathological biopsy (12 cases). The pathological stages (TNM, pTNM) were stage Ⅱ in 3 cases, stage Ⅲ in 20 cases and stage Ⅳ in 3 cases. The distribution source was calculated by computer stereotactic plan system (TPS), and 125I particles were implanted into pancreatic tumor foci under CT guidance. 125I particles with (2.2~3.3) ×107 Mq activity were implanted in a plane 1.0~1.5 cm apart. 125I had the effect of killing tumors within 1.7 cm. Results The pain was relieved completely in 9 patients, partially in 2 cases, and ineffectively in 4 cases. The average pain relief was 3~7 days after operation. 4 cases died of local progression and 2 cases died of distant metastasis. The median survival time of the whole group was 11 months. After 2 months, CT reexamination showed complete remission (CR) in 2 cases; Partial remission (PR) in 13 cases; No change (NC) in 5 cases; The total effective rate (CR + PR) was 57.7%.Three particles (2 patients) migrated into the liver during the 2-month follow-up; There was no serious complication such as pancreatic fistula, pancreatitis, intestinal hemorrhage, intra-abdominal abscess, etc. Conclusion CT-guided radioactive seed implantation in the treatment of pancreatic cancer has less trauma, low incidence of complications, obvious improvement of quality of life, good short-term effect, good palliative and analgesic effect, and is a simple, safe and effective method for the treatment of middle and advanced pancreatic cancer.

Objective to evaluate the clinical value of CT-guided fine needle puncture in the biopsy of pancreas and Vater ampulla.Methods A retrospective study of 106 patients (71 males and 35 females, aged 21-74 years, with an average of 48.7 years) with CT-guided fine needle puncture in the pancreas and Vater ampulla.The symptoms of the patients were abdominal discomfort, abdominal pain, emaciation and fatigue, and some of them had different degrees of skin yellowing and upper abdominal mass.Preoperative CT plain and enhanced scans were performed, and the needle was inserted by anterior approach.The biopsy sites were 70 cases of pancreatic head and ampulla,24 cases of pancreatic body and 12 cases of pancreatic caud.The lesion size was 1.5~5 cm; 22 G (84 cases) and 20 G (22 cases) fine needle puncture were used. The main points of puncture biopsy were to select the best section and needle insertion point, and perform multi-point fan-shaped rapid puncture aspiration. The biopsy specimens were sent to the pathology department for cytopathological examination. Results All 106 cases were punctured safely into the lesions, and the puncture rate of biopsy was 100%. 78 cases were diagnosed as malignant lesions, 18 cases were benign, 3 cases were true negative, and 7 cases were false negative. The total accuracy, sensitivity and specificity of puncture biopsy were 93%, 93% and 100%, respectively. The accuracy rates of CT-guided biopsy for malignant and benign lesions were 94% and 90% (P>0.05). The accuracy rates of large mass lesions [<3 cm 28/32 (88%), ≥3.0 cm 71/74 (96%)] and lesions located in the tail of pancreas (100% in the tail of pancreas, 93% in the ampulla of the head of pancreas, and 92% in the body of pancreas) were slightly higher. The difference in accuracy rates was not statistically significant (P>0.05), and no serious complications were found. Conclusion CT-guided percutaneous fine needle puncture of pancreas and ampulla area was a good diagnosis

Ischemic heart disease is a disease with dominant morbidity and mortality in western countries, and there are about hundreds of millions of patients with ischemic heart disease worldwide [1]. Insufficient coronary blood perfusion leads to corresponding myocardial ischemia and hypoxia is the main problem of advanced ischemic heart disease, so a reasonable treatment strategy is to increase coronary perfusion. At present, traditional revascularization therapy including coronary artery bypass grafting (CABG) and percutaneous transluminal coronary angioplasty (PTCA) can increase coronary perfusion in most patients, which basically achieves the therapeutic purpose, but these therapeutic methods are difficult to achieve the therapeutic purpose in patients with slender coronary artery and diffuse coronary artery. Therapeutic angiogenesis increases blood perfusion in ischemic myocardium by introducing pro-angiogenesis factor into the myocardium to stimulate the formation and maturation of new blood vessels. Among them, vascular endothelial growth factor (VEGF) is the most studied pro-angiogenesis factor in animal experiments and clinical trials [2-4]. Here, VEGF and its therapeutic angiogenesis in ischemic myocardium are reviewed.

Three patients with human immunodeficiency virus (HIV) -1 positive plasma were collected, and primers were designed to target HIV-1 full-length protease gene and partial reverse transcriptase gene (40-250 aa), and the template RNA was amplified by RT-PCR. After the sequence of PCR products was determined, the related sites of HIV-1 drug resistance mutation were analyzed by HIV DB software; The number of CD4+ /CD8+ T lymphocytes in peripheral blood was measured by flow cytometry, and the peripheral blood viral burden was measured by nucleic acid sequence amplification assay (NASBA). The anti-HIV drugs used included didanosine (ddI), stavudine (d4T), ditizhi [AZT (zidovudine) +3TC (lamivudine)], nevirapine (NVP), stonine (EFV) and Jiaxi (IDV).

1. Subjects and methods 8 patients with splenic artery aneurysm, 3 males and 5 females, aged 43-66 years, with an average of 56 years, 4 patients had no discomfort, due to physical examination; Two patients had cholecystitis due to right upper abdominal pain, and CT examination showed that none of the six patients had a history of hypertension, the other two patients had a history of hypertension and diabetes, and one of them had a history of gastrointestinal hemorrhage, which was clinically considered as liver cirrhosis and portal hypertension; Another patient had a history of cerebral stroke and underwent abdominal CT scan due to hypertension. All patients underwent plain CT scan of abdomen and enhanced arterial and venous scans.

multidrug resistance (MDR) is one of the important reasons leading to the failure of chemotherapy for malignant tumors [1]. It has been a hot topic for scholars to accurately and noninvasively detect and monitor the expression of multidrug resistance genes in tumor tissues and realize the individualized treatment of tumors. Some researchers have found [2-5] that the uptake of 99mTc-methoxy isobutyl isonitrile (sestamibi, MIBI) in tumor tissues is related to the expression of some multidrug resistance proteins, which can be used as a noninvasive means to predict the chemotherapy sensitivity of malignant tumors; In this study, three multidrug resistance genes, P-glycoprotein (P-gp), multidrug resistance-associated protein (MRP) and glutathione-S-transferase Pi (GST π), were detected by qualitative and semi-quantitative methods to observe the relationship between their expression and 99mTc-MIBI uptake, and to explore the value of 99mTc-MIBI imaging in predicting chemosensitivity of lung cancer.

The patient, a 24-year-old Han nationality, was admitted to the hospital in August 2003 with intermittent hemoptysis for 3 months. Intermittent hemoptysis began in June 2003 without obvious inducement. It was bright red blood, the volume of hemoptysis per time was about 200~300 ml, accompanied by chest tightness and low fever, and the body temperature fluctuated between 37.4~38℃. The patient had a history of oral and vulvar ulcers for more than 2 years. Physical examination: no obvious cyanosis, mild pestle-shaped fingers and toes, clear breathing sounds in both lungs, regular heart rhythm, grade II systolic murmur can be heard in the pulmonary valve area, the second sound is hyperactive and divided, and the liver and spleen are untouched. There are scattered shallow ulcers in the size of faba beans in the mouth and vulva, with round or elliptical edges, purulent coating on them, flush around them, soft texture and tenderness. Isomorphic reaction was positive. Chest X-ray showed multiple patchy shadows in the middle lobe of the right lung and the lower lobe of the left lung, and enlarged shadows in both hilums.